Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (7): 171-177.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1294

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Genetic Analysis and Gene Mapping of Sorghum Double-grain Mutant Dgs

ZHOU Shi-chen1(), YI Zhi-ben2, WANG Xin-yi1, YANG Xiao-ying1, SUN Li-na1, LUAN Wei-jiang1(), LIANG Shan-shan1()   

  1. 1. College of Life Sciences,Tianjin Normal University/Tianjin Key Laboratory of Animal and Plant Resistance,Tianjin Normal University,Tianjin 300387
    2. School of Chemical Engineering and Technology,North University of China,Taiyuan 030051
  • Received:2021-10-13 Online:2022-07-26 Published:2022-08-09
  • Contact: LUAN Wei-jiang,LIANG Shan-shan E-mail:zschena@163.com;skylss@tjnu.edu.cn;skylwj@tjnu.edu.cn

Abstract:

Grain number per panicle is very important for crop yield. In order to identify and clone the gene controlling grain number per panicle,a double-grain mutant gene of sorghum[Sorghum bicolor(L.)Moench]was located,for laying a foundation for gene cloning and functional analysis and providing evidences for the regulation and analysis of sorghum yield traits. The flower organ phenotypes of wild-type sorghum plant and double-grain mutant were observed by stereoscopy. A F2 population was constructed from the cross between S. bicolor Jin 5 and the double-grain mutant to analyze the inheritance pattern of the mutant and gene mapping. BSA-seq sequencing and map cloning were used for linkage analysis and gene mapping. The results showed that,compared with wild type,the mutant mainly showed an increase in the number of pistil and stamen,ultimately two kernels in one pair of glumes. Genetic analysis indicated that the double-grain trait was controlled by a pair of dominant genes. Gene mapping showed that the target gene was located between markers Indel2930 and SSR7060 on the chromosome 6 of S. bicolor with physical distance of 404 kb. Dgs is a novel sorghum double-grain mutant gene.

Key words: sorghum, double-grain mutant, flower organ, gene mapping, genetic analycis