Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (9): 136-146.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0112

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Genome Construction of Achromobacter 77 and Its Characteristics on Chemotaxis and Antibiotic Resistance

LI Ji-hong1,2(), JING Yu-ling2, MA Gui-zhen1(), GUO Rong-jun2(), LI Shi-dong2   

  1. 1. College of Environmental and Chemical Engineering,Jiangsu Ocean University,Lianyungang 222005
    2. Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193
  • Received:2022-01-25 Online:2022-09-26 Published:2022-10-11
  • Contact: MA Gui-zhen,GUO Rong-jun E-mail:804797175@qq.com;guizhenma@sohu.com;guorj20150620@126.com

Abstract:

Achromobacter sp. 77,the dominant hyphosphere bacterium of Fusarium oxysporum f. sp. cucumerinumFoc),actively migrates along with Foc hyphae growth. To clarify the colonization mechanism of strain 77 in Foc hyphosphere,the whole genome of strain 77 was sequenced by using Illumina Hiseq+PacBio sequencing method,and its characteristics on chemotaxis and antibiotic resistance were examined. The genome of strain 77 was 5 868 070 bp with one chromosome and GC content of 65.89%. Total of 2 696,4 862 and 4 212 genes were annotated against KEGG,COG and GO database,respectively. Gene annotation demonstrated that strain 77 contained multiple important chemotactic genes such as mcp,che and mot in its genome. Annotation of its genome against CARD database showed that it harbored antibiotic resistance efflux pump genes such as drr,emr and gene clusters resistant to multiple antibiotics. Antibiotic sensitivity tests showed that strain 77 was resistant to chloramphenicol,kanamycin and ampicillin at the concentration < 300 µg/mL,and resistant to tetracycline at the concentration < 50 µg/mL,indicating that strain 77 was a multi-drug resistant strain. The chemotactic test indicated that strain 77 showed obviously chemotactic response to 1 mmol/L p-hydroxyphenylacetic acid,salicylic acid,α-ketoglutaric acid,fumaric acid,succinic acid and malic acid. Moreover,the growth of strain 77 was promoted by the above compounds at the concentration of 0.5 mmol/L and by 0.5-1.0 mmol/L of fusaric acid. These results indicated that strain 77 showed chemotaxis toward root or fungal exudates and utilization them,which might be the reason that the abundance of Achromobacter increased in Foc hyphosphere after continuous cultivation of cucumber in Foc infested soil.

Key words: Achromobacter, Fusarium oxysporum f. sp. cucumerinum, whole genome sequencing, chemotaxis, antibiotic resistance