Isolation and Identification of Saline-alkali Tolerant Aspergillus terreus SYAT-1 and Its Activities Against Plant Pathogenic Fungi

doi:10.13560/j.cnki.biotech.bull.1985.2022-0582

Abstract

Abstract:

Microorganisms co-exist and evolve in saline environment for a long time and have unique adaptability and biological properties, which make them the first choice in the microbial improvement of saline land. A saline-alkali tolerant fungal strain SYAT-1 was isolated from a typical soda saline soil in Songyuan city, Jilin province. It was identified by morphological characteristics observation and ITS-rDNA sequence analysis. The growth characteristics at different pH, salt tolerance, antagonistic activity against phytopathogenic fungi and potential pathogenicity of the strain were investigated. The results showed that it was identified as Aspergillus terreus and grew under alkaline（pH=7.0-12.0）medium conditions, with an optimal pH range of 7.0-9.0. It grew well on both 0-17.5 mg/mL NaCl PDA medium and 0-4mg/mL NaHCO₃ PDA medium. Antagonistic phytopathogenic fungi assay results revealed that strain SYAT-1 presented some inhibitory effect on the test phytopathogenic fungi. Potential pathogenicity test results demonstrated that strain SYAT-1 had no potential pathogenicity to the test plants. In sum, A. terreus strain SYAT-1 belongs to a fungus with high salt tolerance characteristics and suppressing phytopathogenic fungi. This study provides new strain resources and theoretical support for the use of microorganisms to improve saline soils and biological control of plant diseases.

Key words: saline-alkali tolerance, Aspergillus terreus, isolation and identification, pathogenic fungus, inhibition

WANG Feng-ting, WANG Yan, SUN Ying, CUI Wen-jing, QIAO Kai-bin, PAN Hong-yu, LIU Jin-liang. Isolation and Identification of Saline-alkali Tolerant Aspergillus terreus SYAT-1 and Its Activities Against Plant Pathogenic Fungi[J]. Biotechnology Bulletin, 2023, 39(2): 203-210.

Figures/Tables 9

Table 1 Collected and isolated fungal strains

土壤编号 Soil No.	采样时间 Collection time	菌株编号 Strain code	土壤编号 Soil No.	采样时间 Collection time	菌株编号 Strain code
SY-1	2020.8.10	SYAT-1	SY-8	2019.9.16	SYAT-4
SY-1	2020.8.10	SYA-2	SY-9	2019.9.16	SYA-1
SY-2	2020.8.10	SYAT-3	SY-9	2019.9.16	SYAO-3
SY-3	2020.8.10	SYTA-1	SY-10	2019.9.16	SYAT-2
SY-3	2020.8.10	SYA-5			SYA-4
SY-4	2020.8.10	SYTH-1			SYAN-1
SY-4	2020.8.10	SYA-3	SY-11	2019.9.16	SYTH-2
SY-5	2020.8.10	SYAO-1	SY-11	2019.9.16	SYAN-2
SY-6	2020.8.10	SYTA-3	SY-12	2019.9.16	SYTA-2
SY-7	2020.8.10	SYA-6	SY-12	2019.9.16	SYA-6
SY-7	2020.8.10	SYAO-2

Fig. 1 Morphological characteristics of strain SYAT-1 A：Colony morphology. B：Conidial peduncle. C：Conidiophore

Fig. 2 Phylogenetic tree constructed based on ITS-rDNA nucleotide sequences Bootstrap values（%）> 50 were displayed. The individual strain in the system tree was represented as：GenBank number-Strain name-Strain number

Fig. 3 Growth of strain SYAT-1 at different pH Different lower letters indicate significant differences at 0.05 level. The same below

Fig. 4 Growth of strain SYAT-1 at 28℃ with different concentrations of NaCl

Fig. 5 Growth of strain SYAT-1 at 28℃ with different concentrations of NaHCO3

Fig. 6 Antagonistic effect of strain SYAT-1 against diffe-rent pathogenic fungi （a）：Sclerotinia sclerotiorum；（b）：Fusarium graminearum；（c）：Botrytis cinerea；（d）：Cochliobolus heterostrophus；（e）：Setosphaeria turcica；（f）：Magnaporthe oryzae

Table2 Inhibition rates of strain SYAT-1 against six plant pathogenic fungi

植物病原真菌Plant pathogenic fungus	抑菌率Inhibition rate/%
Sclerotinia sclerotiorum	34.12±0.81d
Fusarium graminearum	35.29±0.69d
Botrytis cinerea	42.35±0.65c
Cochliobolus heterostrophus	47.89±0.33b
Setosphaeria turcica	50.59±0.74a
Magnaporthe oryzae	21.57±0.73e

Fig. 7 Potential pathogenicity test of the strain SYAT-1 A：Tobacco，tomato and soybean leaves inoculated with S. sclerotiorum. B：Tobacco，tomato and soybean leaves inoculated with A. terreus SYAT-1. C1，C2：Maize leaves inoculated with spore suspension of S. turcica. C3，C4：Maize leaves inoculated with spore suspension of A. terreus SYAT-1. C5：Maize leaves inoculation with 20% Tween-20 as control

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