Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (9): 291-299.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0190
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KANG Ling-yun1,2(), HAN Lu-lu2, HAN De-ping3, CHEN Jian-sheng4, GAN Han-ling4, XING Kai4, MA You-ji1(), CUI Kai2()
Received:
2023-03-03
Online:
2023-09-26
Published:
2023-10-24
Contact:
MA You-ji, CUI Kai
E-mail:18821622269@163.com;yjma@gsau.edu.cn;cuikai@caas.com
KANG Ling-yun, HAN Lu-lu, HAN De-ping, CHEN Jian-sheng, GAN Han-ling, XING Kai, MA You-ji, CUI Kai. Effect of Melatonin on Protecting the Jejunum Mucosal Epithelial Cells from Oxidative Stress Damage[J]. Biotechnology Bulletin, 2023, 39(9): 291-299.
基因Gene | 登录号GenBank ID | 引物序列Primer sequence(5'-3') | 产物长度Product length/bp |
---|---|---|---|
TNF-α | NM_001024860.1 | F: GAGAAGATTCGTGGGCTGAT | 169 |
R: CCTTGTTCTTGTCCTGTGGG | |||
IL-1β | NM_001009465.2 | F: TGATGGCTTATTACAGTGGCAA | 183 |
R: CCATGGCCACAACAACTGAC | |||
IL6 | NM_001009392.1 | F: CCTTCGGTCCAGTTGCCTTCT | 234 |
R: CCAGTGCCTCTTTGCTGCTTTC | |||
β-actin | NM_0010009784.3 | F: GATTCCTATGTGGGCGACGA | 229 |
R: AGGTCTCAAACATGATCTGGGT |
Table 1 Primer sequence information
基因Gene | 登录号GenBank ID | 引物序列Primer sequence(5'-3') | 产物长度Product length/bp |
---|---|---|---|
TNF-α | NM_001024860.1 | F: GAGAAGATTCGTGGGCTGAT | 169 |
R: CCTTGTTCTTGTCCTGTGGG | |||
IL-1β | NM_001009465.2 | F: TGATGGCTTATTACAGTGGCAA | 183 |
R: CCATGGCCACAACAACTGAC | |||
IL6 | NM_001009392.1 | F: CCTTCGGTCCAGTTGCCTTCT | 234 |
R: CCAGTGCCTCTTTGCTGCTTTC | |||
β-actin | NM_0010009784.3 | F: GATTCCTATGTGGGCGACGA | 229 |
R: AGGTCTCAAACATGATCTGGGT |
Fig. 2 FeSO4/H2O2 treatment significantly causing the oxidative damage to mucosal epithelial cells pifevent letters indicate significant dlifference at(P<0.05)level, the same below
抗氧化因子 Antioxidant factor | 不同处理组 Treatment groups | SEM | P | ||||||
---|---|---|---|---|---|---|---|---|---|
CON | FeSO4/H2O2 | MT0.01 | MT0.1 | MT1.0 | MT5.0 | MT10 | |||
MDA | 4.31bc | 5.21a | 4.37bc | 4.17c | 4.37bc | 4.57b | 4.57b | 0.099 | <0.0001 |
LDH | 3.74a | 3.88a | 3.14b | 3.44ab | 3.08b | 3.85a | 3.63a | 0.132 | 0.0011 |
SOD | 1.94ab | 1.90b | 1.90b | 1.97ab | 1.94ab | 1.97ab | 2.00a | 0.022 | 0.068 |
GSH-Px | 3.76bc | 3.60c | 3.96ab | 4.09ab | 4.25a | 4.12ab | 4.34a | 0.104 | 0.002 |
CAT | 216.58a | 167.22b | 165.83b | 174.87b | 163.45b | 179.04b | 169.65b | 6.008 | <0.01 |
Table 2 Effects of pretreatment with different concentrations of melatonin on the expressions of antioxidant factors in mucosal epithelial cells
抗氧化因子 Antioxidant factor | 不同处理组 Treatment groups | SEM | P | ||||||
---|---|---|---|---|---|---|---|---|---|
CON | FeSO4/H2O2 | MT0.01 | MT0.1 | MT1.0 | MT5.0 | MT10 | |||
MDA | 4.31bc | 5.21a | 4.37bc | 4.17c | 4.37bc | 4.57b | 4.57b | 0.099 | <0.0001 |
LDH | 3.74a | 3.88a | 3.14b | 3.44ab | 3.08b | 3.85a | 3.63a | 0.132 | 0.0011 |
SOD | 1.94ab | 1.90b | 1.90b | 1.97ab | 1.94ab | 1.97ab | 2.00a | 0.022 | 0.068 |
GSH-Px | 3.76bc | 3.60c | 3.96ab | 4.09ab | 4.25a | 4.12ab | 4.34a | 0.104 | 0.002 |
CAT | 216.58a | 167.22b | 165.83b | 174.87b | 163.45b | 179.04b | 169.65b | 6.008 | <0.01 |
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