Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (2): 266-276.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0837

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Bacterial Diversity and Structure in Rhizosphere Soil of Citrus Infested with Huanglongbing

LEI Mei-ling1,2(), RAO Wen-hua2, HU Jin-feng2, YUE Qi1,2, WU Zu-jian1, FAN Guo-cheng2()   

  1. 1. College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002
    2. Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013
  • Received:2023-08-28 Online:2024-02-26 Published:2024-03-13
  • Contact: FAN Guo-cheng E-mail:leimeilingfj@126.com;guochengfan@126.com

Abstract:

【Objective】 This study aims to investigate the rhizosphere soil bacterial community of Citrus reticulata plants in the Nanping region of Fujian province, China. The objective is to analyze the impact of Huanglongbing(HLB)disease on the bacterial community and provide a scientific basis for HLB management strategies in the Nanping region.【Method】 We collected root-zone soil samples from both healthy and HLB-affected C. reticulata plants using the five-point sampling method. We used amplicon sequencing technology and bioinformatics methods to investigate the changes in diversity and composition of the bacterial community in the root-zone soil of HLB-affected C. reticulata plants. Additionally, we analyzed the correlation between soil physicochemical factors and the bacterial community.【Result】 The findings of the study demonstrate that the diversity of the bacterial community in the root-zone soil of C. reticulata plants affected by HLB was slightly higher when compared to the microbiota of healthy plants. Specifically, there was a significant increase in the relative abundance of the Proteobacteria, and a significant decrease in the relative abundance of the Actinobacteriota in the root-zone soil of HLB-affected C. reticulata plants. At the genus level, it was observed that the relative abundance of the Roseiarcus and Acidiphilium significantly increased in the bacterial community of HLB-affected C. reticulata plants. Furthermore, these genera presented a significant negative correlation with available potassium, available phosphorus, and organic matter, while showing a significantly positive correlation with pH values. Conversely, the relative abundance of the Conexibacter and Chujaibacter were significantly lower in HLB-affected plants than healthy ones. Additionally, these genera demonstrated a significantly positive correlation with available potassium, available phosphorus, and organic matter, but a significant negative correlation with pH values.【Conclusion】 The infection of the HLB pathogen altered the physicochemical properties of citrus soil and significantly reduced the abundance of beneficial bacteria in the rhizosphere soil. This, in turn, leads to considerable changes in both the diversity and composition of the rhizosphere bacterial community in C. reticulata plants. Concurrently, HLB-infected C. reticulata plants appear to counteract pathogen invasion by recruiting beneficial bacteria that not only fix nitrogen but also promote plant growth in the rhizosphere.

Key words: Huanglongbing, citrus, high-throughput sequencing, rhizosphere soil, bacterial community, differential species, environmental factors