Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (8): 264-274.doi: 10.13560/j.cnki.biotech.bull.1985.2024-0230

Previous Articles     Next Articles

Effects of Compound Microbial Agent on the Growth, Quality and Rhizosphere Environment of Grape

CHE Jian-mei1(), LAI Gong-ti2, LI Si-yu2, GUO Ao-lin2, CHEN Bing-xing1, CHEN Xing1, LIU Bo1(), LAI Cheng-chun2()   

  1. 1. Institute of Resources, Environment and Soil Fertilizer, Fujian Academy of Agricultural Sciences, Fuzhou 350002
    2. Institute of Food Science and Technology,Fujian Academy of Agricultural Sciences, Fuzhou 350002
  • Received:2024-03-08 Online:2024-08-26 Published:2024-09-05
  • Contact: LIU Bo, LAI Cheng-chun E-mail:chejm2002@163.com;fzliubo@163.com;lccisland@163.com

Abstract:

【Objective】Grape is one of the five varieties of fruit trees widely cultivated in China. Long-term excessive application of chemical fertilizer will destroy the microbial ecology, thus affecting the yield and quality of grapes. Compound microbial agent of different strains may be a more active and effective measure to improve the quality of grapes.【Method】The compound microbial agent(Brevibacillus brevis FJAT-0809-GLX, Bacillus velezensis FJAT-55034 and B. brevis FJAT-10623)were used to irrigate the roots, and the effects of the compound microbial agent on grape growth, fruit quality, rhizosphere soil enzymatic activity and diversity of culturable Bacillus were analyzed.【Result】The compound microbial agent promoted the growth of grape leaves, increased the contents of chlorophyll a and carotenoid, and the activities of POD and PPO. The chlorophyll b content, SOD and PPO activities of young grape fruits in the treatment group was 100%, 1.77% and 66.67% higher than that in the control group, respectively. The application of compound microbial agent promoted early ripening, fruit color transformation and quality of grapes. The single fruit weight was 29.06% higher than that of control group, and the soluble solid and soluble protein increased by 7.31% and 94.74%, respectively, compared with the control group. The PPO enzyme activity of ripe grape fruit in the treatment group was significantly higher than that in control group. In addition, the compound microbial agent significantly improved the activities of amylase, catalase, cellulase, sucrase, and the number and species of Bacillus in grape rhizosphere soil. Correlation analysis showed that Bacillus zanthoxyli and Bacillus pseudomycoides promoted grape leaf growth and fruit quality.【Conclusion】The compound microbial agent may improve soil, promote grape growth and increase fruit quality.

Key words: compound microbial agent, Brevibacillus brevis, Bacillus velezensis, grape, quality, soil