Identification and Expression Analysis of UGT Gene Family in Pepper

doi:10.13560/j.cnki.biotech.bull.1985.2024-0269

Abstract

Abstract:

【Objective】The structure and function of UGT gene family members in pepper were identified and analyzed, laying the foundation for subsequent research.【Method】BioEdit, BLASTP and Pfam were used to align and search for UGT members in pepper. CDD and HMMER databases were used to verify conserved domains. ExPASy, Cell PLOC, MAGA X, MG2C, GSDS, and STRING were used to analyze and predict protein physicochemical properties, phylogeny, chromosome localization, gene structure and protein interaction. Transcriptome data and real-time PCR were used to analyze the expression pattern of UGT gene in various organs during the development of various organs.【Result】The prediction results showed that the 140 CaUGTs family members identified from the pepper gene family encoded amino acids in the range of 253-534 and the isoelectric point in the range of 4.7-8.45, most of the CaUGTs were located outside the cell, and a few were located in the plasma membrane and chloroplasts, and were distributed in 17 groups and mapped on 13 chromosomes, of which 25 members were located in chrom12, and all members contained the conserved domains motif 1 and motif 3. They responded to the action elements related to plant growth and development, stress. Real-time quantitative PCR analysis of the expression patterns of UGT gene in pepper under different tissues, stress and hormone response showed that UGT gene was of tissue-specific expression differences, and the expressionof UGT gene significantly increased or decreased under the conditions of ABA, GA3, high and low temperature stress, and CaUGTs members might play different roles in specific stages of development, and may be involved in regulating the defense response under stress in pepper. 【Conclusion】The distribution and structure of the 140 pepper UGT members are diverse, while the members within the group are highly similar, indicating that the CaUGTs gene may respond to abiotic stress during the growth and development of pepper plants.

Key words: pepper, UGT gene family, bioinformatics analysis, membership identification, functional prediction, expression analysis

WU Hui-qin, WANG Yan-hong, LIU Han, SI Zheng, LIU Xue-qing, WANG Jing, YANG Yi, CHENG Yan. Identification and Expression Analysis of UGT Gene Family in Pepper[J]. Biotechnology Bulletin, 2024, 40(9): 198-211.

Figures/Tables 11

References 36

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基因 Gene	正向引物Forward primer（5'-3'）	正向引物Forward primer（5'-3'）
CaUGT31	GCTACAAAGCTGATGCAGGC	GCGAAGACACTCGTACCGAA
CaUGT39	AAGGAGGCCATCGACACAAG	TAGGCAGGATCAAGCTGTGC
CaUGT47	GGGCCTTCTTCCACGAATTT	CGATCCCAAGTCCTCCAAGTT
CaUGT53	GGTTCTTGACTCACAGCGGA	GCGAAAAATGGCCAGCAGAT
CaUGT63	TTAGCTTTGGGAGTGTCGCC	ATCCGCGGGAATCTTGGTTT
CaUGT74	GCTCCTCTTCCCTCTTCCAA	CAAACCGGCGAGACAAAAGA
CaUGT82	CTGAGATGGCATGGGGACTT	GGCAAATTTACAACGTCGTCC
CaUGT92	TGCCGTGTCAATTCGAAAGG	GCAACTTCAACAGCCCATGA
CaUGT127	ATGTCCAAACCCAACTTCACA	CACGGCAGCACCAAAAGTTA
CaUGT128	CCAATGAATGCCAGGTTGGTAG	TGGCCCTCAAATTTTCCCCCAT
CaUGT132	GCCCCTCAAGCAACAATTCT	CACTGGTTGGTCAATGTGCA
CaActin	GGTGACGAGGCTCAATCCAA	CTCTGGAGCCACACGAAGTT

基因 Gene	正向引物Forward primer（5'-3'）	正向引物Forward primer（5'-3'）
CaUGT31	GCTACAAAGCTGATGCAGGC	GCGAAGACACTCGTACCGAA
CaUGT39	AAGGAGGCCATCGACACAAG	TAGGCAGGATCAAGCTGTGC
CaUGT47	GGGCCTTCTTCCACGAATTT	CGATCCCAAGTCCTCCAAGTT
CaUGT53	GGTTCTTGACTCACAGCGGA	GCGAAAAATGGCCAGCAGAT
CaUGT63	TTAGCTTTGGGAGTGTCGCC	ATCCGCGGGAATCTTGGTTT
CaUGT74	GCTCCTCTTCCCTCTTCCAA	CAAACCGGCGAGACAAAAGA
CaUGT82	CTGAGATGGCATGGGGACTT	GGCAAATTTACAACGTCGTCC
CaUGT92	TGCCGTGTCAATTCGAAAGG	GCAACTTCAACAGCCCATGA
CaUGT127	ATGTCCAAACCCAACTTCACA	CACGGCAGCACCAAAAGTTA
CaUGT128	CCAATGAATGCCAGGTTGGTAG	TGGCCCTCAAATTTTCCCCCAT
CaUGT132	GCCCCTCAAGCAACAATTCT	CACTGGTTGGTCAATGTGCA
CaActin	GGTGACGAGGCTCAATCCAA	CTCTGGAGCCACACGAAGTT

项目 Item	蛋白长度 Protein length/aa	分子量 Molecular weight/kD	等电点 pI	不稳定指数 Instability index	疏水指数 GRAVY	带负电残基数 Asp + Glu	带正电残基数 Arg + Lys
分布范围	253-534	20.71×10³-59.65×10³	4.7-8.45	26.52-59.99	-0.334-0.054	29-73	20-64
平均值	450	50.61×10³	5.79	43.35	-0.139	55.53	45.86
亲水蛋白数	—	—	—	—	131	—	—
疏水蛋白数	—	—	—	—	9	—	—
酸性蛋白数	—	—	134	—	—	—	—
碱性蛋白数	—	—	6	—	—	—	—
稳定蛋白	—	—	—	40	—	—	—
不稳定蛋白	—	—	—	100	—	—	—
最小值成员	CaUGT43	CaUGT43	CaUGT101	CaUGT102	CaUGT67	CaUGT15	CaUGT137
最大值成员	CaUGT99	CaUGT99	CaUGT137	CaUGT61	CaUGT49	CaUGT91	CaUGT49

项目 Item	蛋白长度 Protein length/aa	分子量 Molecular weight/kD	等电点 pI	不稳定指数 Instability index	疏水指数 GRAVY	带负电残基数 Asp + Glu	带正电残基数 Arg + Lys
分布范围	253-534	20.71×10³-59.65×10³	4.7-8.45	26.52-59.99	-0.334-0.054	29-73	20-64
平均值	450	50.61×10³	5.79	43.35	-0.139	55.53	45.86
亲水蛋白数	—	—	—	—	131	—	—
疏水蛋白数	—	—	—	—	9	—	—
酸性蛋白数	—	—	134	—	—	—	—
碱性蛋白数	—	—	6	—	—	—	—
稳定蛋白	—	—	—	40	—	—	—
不稳定蛋白	—	—	—	100	—	—	—
最小值成员	CaUGT43	CaUGT43	CaUGT101	CaUGT102	CaUGT67	CaUGT15	CaUGT137
最大值成员	CaUGT99	CaUGT99	CaUGT137	CaUGT61	CaUGT49	CaUGT91	CaUGT49