Effects of Different Concentrations of Melatonin on the Physiological Characteristics of Strawberry Seedlings under High-temperature Stress

doi:10.13560/j.cnki.biotech.bull.1985.2024-0635

Abstract

Abstract:

Objective The impacts of different concentrations of melatonin treatments on the growth and physiological characteristics of strawberry seedlings under high-temperature stress were investigated to establish a theoretical foundation for the advancement of heat-resistant cultivation technology for strawberries. Method ‘Hongyan’ strawberry seedlings were subjected to different concentrations of melatonin via root irrigation and foliar spraying to examine the effects of exogenous melatonin treatments on the growth and physiological characteristics of strawberry seedlings under high-temperature stress. Result The growth of strawberry seedlings was constrained under high temperature stress. However, melatonin treatment alleviated the growth inhibition of strawberry seedlings under high-temperature stress compared to the control. Key parameters such as net photosynthesis rate, transpiration rate, and stomatal conductance of strawberry seedlings showed significant increases. Additionally, the activities of antioxidant enzymes (SOD, POD, and CAT) and proline content were significantly elevated, while hydrogen peroxide content, superoxide anion radical production, relative conductivity and MDA content were notably reduced, with the most pronounced effects observed with a 400 μmoL/L^{treatment. Conclusion Under high-temperature stress, a 400 μmoL/L melatonin treatment improved the ability of strawberry seedlings to withstand heat-induced adversity by enhancing photosynthetic efficiency, boosting antioxidant enzyme activities, maintaining cell membrane stability, and reducing the accumulation of harmful substances.}

Key words: strawberry seedlings, high-temperature stress, melatonin, photosynthesis parameters, physiological characteristics

WU Xia-ming, YANG Min, ZHOU Chen-ping, KUANG Rui-bin, LIU Chuan-he, HE Han, XU Ze, WEI Yue-rong. Effects of Different Concentrations of Melatonin on the Physiological Characteristics of Strawberry Seedlings under High-temperature Stress[J]. Biotechnology Bulletin, 2025, 41(3): 181-189.

Figures/Tables 6

References 36

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处理 Treatment	地上部分鲜重 Fresh weight of aboveground parts/g	地下部分鲜重 Fresh weight of underground parts/g	地上部分干重 Dry weight of aboveground parts/g	地下部分干重 Dry weight of underground parts/g
CK	3.85±0.19 c	4.32±0.15 b	0.38±0.02 bc	0.43±0.03 b
100 MT	4.05±0.22 bc	4.32±0.23 b	0.38±0.02 bc	0.44±0.02 b
200 MT	3.81±0.23 c	4.22±0.28 b	0.36±0.02 c	0.41±0.03 b
300 MT	4.29±0.17 b	4.56±0.19 ab	0.42±0.03 b	0.46±0.04 b
400 MT	4.98±0.38 a	4.87±0.14 a	0.50±0.03 a	0.52±0.03 a
500 MT	4.20±0.23 bc	4.35±0.11 b	0.38±0.03 bc	0.44±0.01 b

处理 Treatment	地上部分鲜重 Fresh weight of aboveground parts/g	地下部分鲜重 Fresh weight of underground parts/g	地上部分干重 Dry weight of aboveground parts/g	地下部分干重 Dry weight of underground parts/g
CK	3.85±0.19 c	4.32±0.15 b	0.38±0.02 bc	0.43±0.03 b
100 MT	4.05±0.22 bc	4.32±0.23 b	0.38±0.02 bc	0.44±0.02 b
200 MT	3.81±0.23 c	4.22±0.28 b	0.36±0.02 c	0.41±0.03 b
300 MT	4.29±0.17 b	4.56±0.19 ab	0.42±0.03 b	0.46±0.04 b
400 MT	4.98±0.38 a	4.87±0.14 a	0.50±0.03 a	0.52±0.03 a
500 MT	4.20±0.23 bc	4.35±0.11 b	0.38±0.03 bc	0.44±0.01 b

处理 Treatment	蒸腾速率 Transpiration rate/（mmol·m^-2·s^-1）				净光合速率 Net photosynthesis rate/（µmol·m^-²·s^-¹）				胞间CO₂浓度 Intercellular CO₂ concentration/（µmol·mol^-1）				气孔导度 Stomatal conductance/（mmol·m^-²·s^-¹）
处理 Treatment	0 d	3 d	6 d	9 d	0 d	3 d	6 d	9 d	0 d	3 d	6 d	9 d	0 d	3 d	6 d	9 d
CK	1.64±0.27 a	0.45±0.15 b	0.11±0.02 b	0.05±0.01 c	12.54±2.31 a	3.16±0.81 d	0.13±0.01 c	-2.16±0.48 b	397.28±16.45 a	387.03±19.77a	365.85±4.42 a	355.85±6.34 a	56.41±4.75 a	15.20±2.66 c	5.63±0.93 c	1.07±0.47 c
100 MT	1.66±0.35 a	0.80±0.23 ab	0.15±0.03 ab	0.07±0.01 bc	13.74±3.11 a	5.32±1.04 c	0.20±0.03 b	-1.87±0.33 b	387.94±14.47 a	372.02±13.80 ab	339.89±15.43 ab	333.23±8.29 bc	54.91±5.83 a	23.81±6.66 bc	8.41±1.32 ab	1.91±0.37 b
200 MT	1.66±0.17 a	0.69±0.08 ab	0.16±0.03 a	0.07±0.01 bc	12.67±1.84 a	6.22±0.37 bc	0.22±0.02 ab	-1.82±0.40 b	396.26±22.65 a	368.55±21.79 ab	349.96±19.91 ab	339.96±7.64 b	58.97±5.24 a	25.27±2.98 ab	7.86±0.72 bc	2.19±0.37 ab
300 MT	1.63±0.44 a	0.93±0.30 a	0.16±0.03 a	0.06±0.01 bc	13.34±2.25 a	7.97±0.54 a	0.22±0.02 ab	-1.72±0.17 b	391.42±21.64 a	344.81±33.55 bc	339.93±24.13 ab	329.93±10.60 bc	55.26±3.93 a	29.37±6.18 a	8.61±1.06 ab	2.34±0.22 ab
400 MT	1.74±0.47 a	0.99±0.20 a	0.17±0.02 a	0.10±0.01 a	13.07±1.67 a	7.26±1.61 ab	0.26±0.01 a	-0.53±0.16 a	389.58±21.87 a	328.02±18.17 c	325.84±9.84 b	319.17±7.05 c	60.38±5.17 a	36.70±5.55 a	9.81±1.49 ab	2.81±0.59 a
500 MT	1.71±0.36 a	0.97±0.19 a	0.16±0.01 a	0.08±0.01 ab	12.94±2.02 a	6.81±0.99 abc	0.25±0.05 ab	-0.55±0.12 a	387.53±26.74 a	350.93±11.19 abc	336.22±5.52 b	324.88±11.34 bc	60.24±6.43 a	37.18±5.45 a	10.18±1.92 a	2.31±0.41 ab

处理 Treatment	蒸腾速率 Transpiration rate/（mmol·m^-2·s^-1）				净光合速率 Net photosynthesis rate/（µmol·m^-²·s^-¹）				胞间CO₂浓度 Intercellular CO₂ concentration/（µmol·mol^-1）				气孔导度 Stomatal conductance/（mmol·m^-²·s^-¹）
处理 Treatment	0 d	3 d	6 d	9 d	0 d	3 d	6 d	9 d	0 d	3 d	6 d	9 d	0 d	3 d	6 d	9 d
CK	1.64±0.27 a	0.45±0.15 b	0.11±0.02 b	0.05±0.01 c	12.54±2.31 a	3.16±0.81 d	0.13±0.01 c	-2.16±0.48 b	397.28±16.45 a	387.03±19.77a	365.85±4.42 a	355.85±6.34 a	56.41±4.75 a	15.20±2.66 c	5.63±0.93 c	1.07±0.47 c
100 MT	1.66±0.35 a	0.80±0.23 ab	0.15±0.03 ab	0.07±0.01 bc	13.74±3.11 a	5.32±1.04 c	0.20±0.03 b	-1.87±0.33 b	387.94±14.47 a	372.02±13.80 ab	339.89±15.43 ab	333.23±8.29 bc	54.91±5.83 a	23.81±6.66 bc	8.41±1.32 ab	1.91±0.37 b
200 MT	1.66±0.17 a	0.69±0.08 ab	0.16±0.03 a	0.07±0.01 bc	12.67±1.84 a	6.22±0.37 bc	0.22±0.02 ab	-1.82±0.40 b	396.26±22.65 a	368.55±21.79 ab	349.96±19.91 ab	339.96±7.64 b	58.97±5.24 a	25.27±2.98 ab	7.86±0.72 bc	2.19±0.37 ab
300 MT	1.63±0.44 a	0.93±0.30 a	0.16±0.03 a	0.06±0.01 bc	13.34±2.25 a	7.97±0.54 a	0.22±0.02 ab	-1.72±0.17 b	391.42±21.64 a	344.81±33.55 bc	339.93±24.13 ab	329.93±10.60 bc	55.26±3.93 a	29.37±6.18 a	8.61±1.06 ab	2.34±0.22 ab
400 MT	1.74±0.47 a	0.99±0.20 a	0.17±0.02 a	0.10±0.01 a	13.07±1.67 a	7.26±1.61 ab	0.26±0.01 a	-0.53±0.16 a	389.58±21.87 a	328.02±18.17 c	325.84±9.84 b	319.17±7.05 c	60.38±5.17 a	36.70±5.55 a	9.81±1.49 ab	2.81±0.59 a
500 MT	1.71±0.36 a	0.97±0.19 a	0.16±0.01 a	0.08±0.01 ab	12.94±2.02 a	6.81±0.99 abc	0.25±0.05 ab	-0.55±0.12 a	387.53±26.74 a	350.93±11.19 abc	336.22±5.52 b	324.88±11.34 bc	60.24±6.43 a	37.18±5.45 a	10.18±1.92 a	2.31±0.41 ab

处理 Treatment	SOD活性SOD enzyme activity/（U·g^-1）				CAT活性CAT enzyme activity/（U·g^-1）				POD活性POD enzyme activity/（U·g^-1）
处理 Treatment	0 d	3 d	6 d	9 d	0 d	3 d	6 d	9 d	0 d	3 d	6 d	9 d
CK	72.36±3.47 a	114.31±3.83 b	125.22±4.43 c	84.83±3.43 b	96.61±7.50 a	133.35±5.89 e	159.58±3.98 c	102.93±5.71 c	37.28±4.64 a	55.33±3.06 d	76.48±4.62 d	55.33±3.06 d
100 MT	78.54±4.88 a	115.21±11.88 b	132.31±4.56 bc	86.30±4.63 b	103.31±6.23 a	150.86±9.72 de	197.23±4.17 b	109.26±5.98 bc	38.53±5.24 a	79.33±4.16 c	95.87±7.64 c	54.67±5.67 cd
200 MT	81.64±7.64 a	130.01±7.78 a	138.60±5.06 ab	96.62±2.18 a	106.87±8.79 a	183.27±9.08 c	210.17±7.74 b	116.18±3.47 ab	41.69±3.16 a	80.67±7.02 c	104.58±3.21 c	57.38±3.06 c
300 MT	76.48±3.06 a	126.73±2.01 ab	133.15±5.93 bc	96.83±1.17 a	105.47±5.84 a	156.47±5.71 d	206.17±21.96 b	110.78±4.31 bc	43.63±5.14 a	90.66±7.57 b	129.34±8.08 b	61.65±3.51 bc
400 MT	80.54±5.71 a	136.47±10.71 a	146.60±4.58 a	102.58±8.22 a	110.64±13.58 a	239.07±26.25 a	265.39±13.85 a	119.82±5.77 a	47.28±7.38 a	107.64±3.51 a	146.42±8.74 a	69.43±4.16 a
500 MT	79.36±4.87 a	126.04±5.05 ab	140.92±6.20 ab	97.91±2.26 a	109.82±11.58 a	210.62±7.74 b	251.55±8.55 a	116.71±4.10 ab	43.85±5.54 a	93.32±5.03 b	135.46±5.86 ab	66.32±4.04 ab