Screening， Indentification and Promotion Performance Investigation of Tobacco Growth-promoting Rhizobacteria

doi:10.13560/j.cnki.biotech.bull.1985.2024-0774

Abstract

Abstract:

Objective In order to provide important microbial fertilizer germplasm resources for promoting plant growth and improving farmland soil environment, the growth promoting bacteria with multiple functions were screened out in the rhizosphere of tobacco plants, and their effects on promoting plant growth and development were studied. Method The strains were screened from the rhizosphere of tobacco plants. The IAA production of these strains was determined by Salkowski colorimetry,and their phosphorus- and potassium-dissolving abilities were determined by molybdenum antimony resistance colorimetry and flame photometry. The dominant growth-promoting strains were screened by morphological, physiological and biochemical identification and 16S rDNA molecular identification. The optimal growth-promoting conditions were studied by orthogonal test, and the growth-promoting effect was studied by pot experiment. Result An efficient and multifunctional growth-promoting strain Arthrobacter woluwensis DY8 was screened. Its IAA-producing ability reached 36.7 mg/L, potassium-dissolving and inorganic phosphorus-dissolving ability reached 72.3 mg/L and 148.6 mg/L, respectively, and it antagonized pathogenic bacteria. Among these, the IAA production capacity reached its highest when the liquid volumewas 30 mL/250 mL, the pHwas 8, peptone was used as the nitrogen source, and glucose was used as the carbon source. In the tobacco potted experiment, after inoculating with strain DY8, in comparison with the control group, the contents of IAA, available phosphorus and potassium in tobacco soil were significantly augmented and the biomass and photosynthesis of tobacco also increased. Principal Component Analysis (PCA) results indicate that DY8 enhanced tobacco biomass by increasing the IAA content in the soil. In pot experiments with bok choy and wheat, DY8 treatment improved root structure, promoted root development, and increased above-ground biomass. Conclusion DY8 is a multifunctional growth-promoting bacterium that generates IAA, solubilizes phosphorus and potassium. It has a fine growth-promoting effect on economic crops, vegetable crops.

Key words: tobacco, plant growth-promoting rhizobacteria, Arthrobacter woluwensis, Chinese cabbage, wheat

MA Yao-wu, ZHANG Qi-yu, YANG Miao, JIANG Cheng, ZHANG Zhen-yu, ZHANG Yi-lin, LI Meng-sha, XU Jia-yang, ZHANG Bin, CUI Guang-zhou, JIANG Ying. Screening， Indentification and Promotion Performance Investigation of Tobacco Growth-promoting Rhizobacteria[J]. Biotechnology Bulletin, 2025, 41(3): 271-281.

Figures/Tables 11

References 36

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处理编号 Treatment code	C源 C source	N源 N source	pH	装液量 Liquid volume/（mL·250 mL^-1）	产IAA能力 IAA-producing capacity/（mg·L^-1）	解钾能力 K dissolvability/（mg·L^-1）
1	麦芽糖	尿素	6	30	10.42	34.35
2	麦芽糖	酵母粉	8	50	36.67	8.27
3	麦芽糖	蛋白胨	7	70	36.26	57.83
4	葡萄糖	尿素	8	70	0.00	8.67
5	葡萄糖	酵母粉	7	30	62.93	60.33
6	葡萄糖	蛋白胨	6	50	34.48	83.10
7	蔗糖	尿素	7	50	5.60	24.67
8	蔗糖	酵母粉	6	70	19.99	52.33
9	蔗糖	蛋白胨	8	30	59.09	43.72
产IAA能力
K1	27.79	5.34	21.63	44.15
K2	32.47	39.87	31.92	25.58
K3	28.23	43.28	34.93	18.75
R	4.68	37.93	13.30	25.39
最优组合	葡萄糖	蛋白胨	7	30
影响力顺序	氮源>装液量>pH>碳源
解钾能力
K1	33.48	22.56	56.59	46.13
K2	50.70	40.31	20.22	38.68
K3	40.24	61.55	47.61	39.61
R	17.22	38.99	36.37	7.45
最优组合	葡萄糖	蛋白胨	6	30
影响力顺序	氮源>pH>碳源>装液量

处理编号 Treatment code	C源 C source	N源 N source	pH	装液量 Liquid volume/（mL·250 mL^-1）	产IAA能力 IAA-producing capacity/（mg·L^-1）	解钾能力 K dissolvability/（mg·L^-1）
1	麦芽糖	尿素	6	30	10.42	34.35
2	麦芽糖	酵母粉	8	50	36.67	8.27
3	麦芽糖	蛋白胨	7	70	36.26	57.83
4	葡萄糖	尿素	8	70	0.00	8.67
5	葡萄糖	酵母粉	7	30	62.93	60.33
6	葡萄糖	蛋白胨	6	50	34.48	83.10
7	蔗糖	尿素	7	50	5.60	24.67
8	蔗糖	酵母粉	6	70	19.99	52.33
9	蔗糖	蛋白胨	8	30	59.09	43.72
产IAA能力
K1	27.79	5.34	21.63	44.15
K2	32.47	39.87	31.92	25.58
K3	28.23	43.28	34.93	18.75
R	4.68	37.93	13.30	25.39
最优组合	葡萄糖	蛋白胨	7	30
影响力顺序	氮源>装液量>pH>碳源
解钾能力
K1	33.48	22.56	56.59	46.13
K2	50.70	40.31	20.22	38.68
K3	40.24	61.55	47.61	39.61
R	17.22	38.99	36.37	7.45
最优组合	葡萄糖	蛋白胨	6	30
影响力顺序	氮源>pH>碳源>装液量

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