Studies on the Growth-promoting Effect of Bacillus Strain from Rhizosphere in Ionic Rare Earth Ores

doi:10.13560/j.cnki.biotech.bull.1985.2024-0292

Abstract

Abstract:

【Objective】 To explore innovative ways for the development of microbial fertilizers, rhizosphere microbial resources of plants are being mined from ionic rare earth mines, of which growth-promoting bacteria with multiple functions were isolated and identified, and their biological characteristics and promoting effects were also investigated. 【Method】 The strain DW019 isolated from the rhizosphere in an ionic rare earth mining area in Gannan of Jiangxi province was identified based on 16S rRNA gene sequence analysis and culture characteristics, classifying its genus and species. Its growth-promoting characteristics was preliminarily determined by the detection of production of IAA, iron carriers, and ammonia. The antagonistic effect of the strain against typical soil-borne disease causative organisms was measured by using the plate-confrontation method. Finally, the growth indexes, chlorophyll, antioxidant enzyme activities, and nutritional quality of lettuce（Lactuca sativa）were measured by potting experiments to determine the growth-promoting effect of strain DW019 on lettuce. 【Result】 The strain was identified as Bacillus cereus DW019, with the ability of producing IAA, iron carrier and ammonia. The strain showed strong inhibition effects against Thanatephorus cucumeris, Fusarium moniliforme Sheldon and Alternaria alternata（Fries）Keissler. Compared to the control group, the above-ground part biomass, biological indices of potted lettuce were significantly improved by the addition of different concentrations of DW019, respectively, and its chlorophyll content, water content, vitamin C, soluble sugars, malondialdehyde（MDA）and peroxidase（POD）were also significantly promoted. 【Conclusion】 Bacillus cereus DW019 is a multifunctional plant growth-promoting rhizobacterium with phytohormone-producing and biocontrol ability, which has strong inhibitory effect on a variety of soil-borne disease-causing bacteria, and significantly promotes plant growth and improves plant quality.

Key words: ionic rare earth ore, biological control, plant growth-promoting rhizobacteria, Bacillus, antagonism, growth-promoting effect, lettuce

LI Xi, BIAN Zi-jun, NING Zhou-shen, LIU Hong-yu, ZENG Bing, DONG Wei. Studies on the Growth-promoting Effect of Bacillus Strain from Rhizosphere in Ionic Rare Earth Ores[J]. Biotechnology Bulletin, 2024, 40(11): 259-268.

Figures/Tables 8

References 34

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分组Group	施药后 7 d 的药害情况Damage at 7 d after application
CK	生长正常、叶色绿，无药害 Normal growth, green leaf color, and no drug damage
S1	生长正常、叶色绿，无药害 Normal growth, green leaf color, and no drug damage
S2	生长正常、叶色绿，无药害 Normal growth, green leaf color, and no drug damage
S3	生长正常、叶色绿，无药害 Normal growth, green leaf color, and no drug damage
S4	生长正常、叶色绿，无药害 Normal growth, green leaf color, and no drug damage

分组Group	施药后 7 d 的药害情况Damage at 7 d after application
CK	生长正常、叶色绿，无药害 Normal growth, green leaf color, and no drug damage
S1	生长正常、叶色绿，无药害 Normal growth, green leaf color, and no drug damage
S2	生长正常、叶色绿，无药害 Normal growth, green leaf color, and no drug damage
S3	生长正常、叶色绿，无药害 Normal growth, green leaf color, and no drug damage
S4	生长正常、叶色绿，无药害 Normal growth, green leaf color, and no drug damage

分组 Group	地上部分生物量 Above-ground biomass/g	地下部分生物量 Underground biomass/g	茎粗 Stem thickness/cm	子叶数 Cotyledons/pieces	株高 Plant height/cm
CK	16.74±3.11a	2.74±0.71a	3.04±0.36a	14.40±2.30a	11.86±0.67a
S1	17.65±1.66a	3.21±0.86ab	3.22±0.14a	15.40±1.34a	12.78±1.139ab
S2	25.22±5.41b	4.44±1.18b	4.02±0.31b	16.20±0.30a	13.08±0.61ab
S3	42.72±3.46c	6.14±1.19c	4.94±0.31c	19.80±1.48b	13.81±1.15b
S4	55.13±5.85d	7.51±0.63c	5.26±0.11c	21.60±0.55b	14.14±1.09b

分组 Group	地上部分生物量 Above-ground biomass/g	地下部分生物量 Underground biomass/g	茎粗 Stem thickness/cm	子叶数 Cotyledons/pieces	株高 Plant height/cm
CK	16.74±3.11a	2.74±0.71a	3.04±0.36a	14.40±2.30a	11.86±0.67a
S1	17.65±1.66a	3.21±0.86ab	3.22±0.14a	15.40±1.34a	12.78±1.139ab
S2	25.22±5.41b	4.44±1.18b	4.02±0.31b	16.20±0.30a	13.08±0.61ab
S3	42.72±3.46c	6.14±1.19c	4.94±0.31c	19.80±1.48b	13.81±1.15b
S4	55.13±5.85d	7.51±0.63c	5.26±0.11c	21.60±0.55b	14.14±1.09b

指标 Norm	子叶数 Cotyledons	株高 Plant height	茎粗 Stem thickness	含水量 Water content	地上部生物量 Above-ground biomass	地下部生物量 Underground biomass	土壤有机质 Soil organic matter
子叶数Cotyledons	1
株高Plant height	0.613**	1
茎粗Stem thickness	0.867**	0.621**	1
含水量Water content	0.547**	0.456*	0.635**	1
地上部生物量 Above-ground biomass	0.915**	0.675**	0.951**	0.714**	1
地下部生物量 Underground biomass	0.894**	0.588**	0.934**	0.555**	0.930**	1
土壤有机质 Soil organic matter	-0.615**	-0.559**	-0.693**	-0.312	-0.684**	-0.616**	1