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Antioxidant Activity of Solvent Extracts of Xinjiang Propolis and Determination of Quercetin and Chrysin Propolis Buwihelqem Ababekri, Mutallip Amet Aminigul, Mamat Nizam Ehet, Yimit Rahman Biotechnology Bulletin    2013, 0 (2): 163-171.   Abstract （465）      PDF（pc） （2778KB）（49844）       Knowledge map    Save To study the DPPH free radical scavenging activity and anti-lipid peroxidation activity of Kuqa and Yili, and determine the content of the Quercetin and Chrysin in Xinjiang Yili propolis. Different extracts of raw propolis were extracted by four kinds of solvents, taking tea polyphenol as comparison; measure Kuqa and Yili, Xinjiang propolis extracts antioxidant activity in removal of DPPH（1, 1-Diphenyl- 1-picrylhydrazyl）free radicals and antioxidant properties of linoleic acid peroxidation. With 70% ethanol and methanol as solvent to extract Quercetin and Chrysin from Xinjiang Yili propolis, using HPLC（high performance liquid chromatography）to determine the content of the extracted Quercetin and Chrysin, the detection wavelength was 370 nm and 268 nm, respectively. Result showed that propolis different extracts in different concentrations has different scavenging free radical DPPH capacity, in partial experimental groups’ scavenging capacity is even surpassed to same concentration of tea polyphenol group; has antioxidant activity to linoleic acid, in which Yili propolis has higher antiperoxidation activity. The content of Quercetin was the lowest in the without defatted ethanol extracts（2.2950 mg/g）, the highest in the defatted methanol extracts（2.8150 mg/g）；The content of Chrysin was the lowest in the without defatted methanol extracts（60.72 mg/g）, the highest in the defatted methanol extracts（74.37 mg/g）. The Kuqa and Yili propolis has a certain extent of antioxidant capacity, this DPPH method is fast, sensitive and simple, this results showed that the Xinjiang propolis is a natural antioxidant, and waiting for further study and utilization; HPLC analysis showed that Quercetin and Chrysin the active ingredients of propolis samples from Xinjiang Yili were detected. Reference | Related Articles | Metrics

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Transcriptome and RNA-Seq Technology Zhang Chunlan, Qin Zijuan, Wang Guizhi, Ji Zhibin, Wang Jianmin Biotechnology Bulletin    2012, 0 (12): 51-56.   Abstract （1289）      PDF（pc） （1420KB）（8279）       Knowledge map    Save The transcriptome is the complete set of transcripts for certain type of cells or tissues in a specific developmental stage orphysiological condition. Transcriptome analysis can reveal the organism's gene expressing level, structural variation, discovery of new genes. Theresearch methods and platforms of transcriptome are undergoing rapid changes. And bioinformatics analysis is also improved gradually. RNASeqas a new research method, it can be more quickly and afford more accurate transcriptome’s information while using the Next-generationSequencing（NGS）technology. This article compares several main methods and platforms of transcriptome in recent years, and review theprinciple, purpose, steps, bioinformatics’ analysis and applications in related fields of RNA-Seq. This will be afford useful reference for relatedresearch. Reference | Related Articles | Metrics

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The Cancer-related Bioinformatics Databases Yang Jian, Cai Haoyang Biotechnology Bulletin    2015, 31 (11): 89-101.   DOI: 10.13560/j.cnki.biotech.bull.1985.2015.11.010 Abstract （1007）   HTML    PDF（pc） （1223KB）（5985）       Knowledge map    Save Malignant tumor has become one of the major diseases that takes seriously risks to human health. In recent years, the rapid development of high-throughput detection technology has become an important means in cancer research. In this way, the cancer genomics data accumulated rapidly. These data is important for the research of mechanisms of tumor initiation and development. Massive biological data management and mining have become the foundation and an important field in cancer research. This article describes the frequently used bioinformatics databases of human tumors, including comprehensive databases, databases of genomics, transcriptomics, proteomics, epigenetics, etc. Here we sum up the status quo of database development in China and abroad, and discuss the existing problems to assist current research. Reference | Related Articles | Metrics

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Research Advances on the Mechanism of Glyceraldehydes-3-phosphate Dehydrogenase in Plant Lu Qian, Mi Xiaoju, Cui Jizhe Biotechnology Bulletin    2013, 0 (8): 1-6.   Abstract （674）      PDF（pc） （1372KB）（4603）       Knowledge map    Save Glycolysis and the Calvin cycle are critical metabolic pathways of energy supply and carbon fixation in plants. Glyceraldehyde-3-phosphate dehydrogenase plays a central role in glycolysis and the Calvin cycle. Recent research results disclosed that the function and regulation of GAPDH turned out to be quite diverse and complex. This article provides an overview of the latest progress on the GAPDH type and function, the specificity interaction of GAPDH and NAD（P）, PRK/GAPDH/CP12 complex and regulation, and the mechanism of GAPDH response to stress in plants. Reference | Related Articles | Metrics

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Research Progresses in the Synthetic Biology of Terpenoids SUN Li-chao, LI Shu-ying, WANG Feng-zhong, XIN Feng-jiao Biotechnology Bulletin    2017, 33 (1): 64-75.   DOI: 10.13560/j.cnki.biotech.bull.1985.2017.01.007 Abstract （1208）   HTML    PDF（pc） （1825KB）（4584）       Knowledge map    Save The terpenoids represent the largest class of natural products with biological activities of antitumor and anti-allergy,thus they have been widely applied in the area of food,cosmetics and medical health,presenting huge potential and broad market prospects. Recent years,researchers applied functional genomics and metabonomics approaches to deeply study the biosynthesis pathways of terpenoids,providing tons of data for their synthetic biology. The construction of engineered yeasts using synthetic biology enabled the efficient synthesis of multi-target terpenoids,and highly improved the overall production level. Thus,the synthetic biology approach is expected to be an efficient way of producing plant-derived terpenoids. First,we introduced the concept of synthetic biology,summarized the important functions and applications of plant-derived terpenoids,briefly reviewed the biosynthesis pathways,and concluded the alternative production ways. Then,we discussed the design strategies of synthetic biology for terpenoids thoroughly. Finally,we elaborated the advances on the biosynthetic biology of varied terpenes with common terpenes as the studied cases. Reference | Related Articles | Metrics

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Progress on the Studies and Application of Clostridium butyricum Zhang Shanting Shi Yan Zhang Shuli Wang Haikuan Biotechnology Bulletin    2013, 29 (9): 27-33.   Abstract （720）      PDF（pc） （1194KB）（4479）       Knowledge map    Save Clostridium butyricum, as a probiotic has been studied widely, is a spore-forming, gram-positive anaerobe that is found in intestines of healthy animals and humans. As a novel spore-forming probiotic, C. butyricum can withstand the low pH, heat and many antibiotic compared with non-spore forming probiotics, and exert different health effects in host, such as regulation of intestinal microbial homeostasis, stabilization of the gastrointestinal barrier function, enzymatic activity inducing absorption and nutrition, immunomodulatory effects, improvement of the animal production performance. So it will be used more widely. In this paper, we reviewed advances in the biological function, the fermentation technology and the application in clinic and feed additive of C. butyricum. Some advices were also put forward for further research of C. butyricum.Key words: Clostridium butyricum Probiotic Fermentation Clinic Feed additive Reference | Related Articles | Metrics

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Research of Reactive Oxygen Species in Plants and Its Application on Stress Tolerance Xue Xin, Zhang Qian, Wu Jinxia, Biotechnology Bulletin    2013, 29 (10): 6-11.   Abstract （916）      PDF（pc） （1177KB）（4321）       Knowledge map    Save The ROS（Reactive oxygen species）is a plant byproduct of aerobic metabolism，environmental stress also makes a large amount of a ROS accumulate in plant cells. Low concentrations of ROS can exist as a signaling molecule to induce the expression of defense genes and plant adaptation to the environment reaction. When the stress force in plant cells produce large amounts of reactive oxygen species, it will cause damage to intracellular macromolecules and other components, to impede normal plant metabolism and growth, and even death. Plants existence of active oxygen scavenging mechanism, the balance can be maintained within a certain range of reactive oxygen species. Studies have shown that the plants active oxygen scavenging mechanism can improve the resistance of plants. In this paper, an overview of the current dynamic of Reactive Oxygen Species, at the same time explore the application of plant active oxygen scavenging mechanism in improving plant stress resistance. Reference | Related Articles | Metrics

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Application Research Progress of Proteomics Yin Wen, Fu Xu, Li Ping Biotechnology Bulletin    2014, 30 (1): 32-38.   Abstract （1209）      PDF（pc） （1201KB）（4273）       Knowledge map    Save Proteomics is an emerging discipline for studying proteins composition and function in a type of cell, tissue or body fluids in a large-scale, high-throughput and systematic level. While genes determine the level of protein, but the level of gene expression can not represent the intracellular reactive protein levels. Proteomic analysis is a complement to the study of translation and modification and also an indispensable tool for a comprehensive understanding of genome expression. The development of proteomic technologies has greatly promoted the progress of proteomic research, and it has been widely used in various research fields.This paper revieweded the proteomic technologies and the applications in various fields are also briefly reviewed. Finally, some future issues are presented. Reference | Related Articles | Metrics

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Research Progress of Bacillus subtilis Expression System and Its Promoter Regulatory Elements Yu Xiaoxia, Tian Jian, Liu Xiaoqing, Wu Ningfeng Biotechnology Bulletin    2015, 31 (2): 35-44.   DOI: 10.13560/j.cnki.biotech.bull.1985.2015.02.005 Abstract （669）   HTML    PDF（pc） （1414KB）（4011）       Knowledge map    Save As a Gram-positive bacteria, Bacillus subtilis is an attractive host for the production of heterologous secretory proteins for several reasons：it is non-pathogenic and the capable of secreting functional extracellular proteins directly to the culture medium, a great deal of vital information concerning large scale fermentation and production technology. One of the key factors for achieving high-level expression of heterologous proteins is the use of a strong and control promoter. In present, the promoters of Bacillus subtilis can be classified into three categories：constitutive promoters, inducer-specific promoters and autoinducible promoters. This paper described the advantages and disadvantages of Bacillus subtilis expression system and the classification of promoters. At the same time, we summarized the methods of the amplification of new promoters, which provided a foundation for improving Bacillus subtilis expression systems and the industrial production of heterologous proteins . Reference | Related Articles | Metrics

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Principle of Whole Genome Amplification Technology and Its Progress Pan Xiaoming, Liang Xingguo Biotechnology Bulletin    2014, 30 (12): 47-54.   DOI: 10.13560/j.cnki.biotech.bull.1985.2014.12.008 Abstract （592）      PDF（pc） （2031KB）（3870）       Knowledge map    Save The rapid developments of DNA array and next generation sequencing technologies had greatly promoted molecular biology techniques to be used in various fields. However, the limited quantity and quality of sample DNA may affect large scale genetic analyses. To overcome these problems, the whole genome amplification(WGA)technology was employed to amplify trace amounts of genomic DNA to sufficient quantity to meet the requirements of high throughput analyses. The highly efficient and reliable WGA methods also helped to realize large scale of genetic analyses based on even single cell. Moreover, as the single cell level research continues, more efficient WGA approaches were developed. This paper summarized the general WGA methods frequently used before and newly developed methods on basic principle level as well as the advantages and disadvantages of each method, which helped to make good use of the powerful WGA techniques. Reference | Related Articles | Metrics

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Advances and Applications on Methodology of 16S rRNA Sequencing in Gut Microbiota Analysis Li Dongping, Guo Mingzhang, Xu Wentao Biotechnology Bulletin    2015, 31 (2): 71-77.   DOI: 10.13560/j.cnki.biotech.bull.1985.2015.02.010 Abstract （504）   HTML    PDF（pc） （1090KB）（3835）       Knowledge map    Save 16S rRNA sequencing is one of the high-throughput-sequencing-based methods used in gut microbiota analysis. Almost all the bacterial species in gut microbiota can be quantified through 16S rRNA sequencing, which has made this method into the mainstream. Two issues are very important in the application of 16S rRNA sequencing：sequencing strategy and bioinformatic analysis. In this review, three aspects of the sequencing strategy, including sequencing platform, sequencing region, and data size were discussed. While on bioinformatic analysis, the advance in sequences cluster and annotation, microbiota structure analysis, key taxa screening and functional analysis were reviewed here. Reference | Related Articles | Metrics

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Research Progress of Structural Characteristics and Functions of Calcium-dependent Protein Kinases in Plants Jiang Shanshan, Zhang Dan, Kong Xiangpei, Zhou Yan, Li Dequan Biotechnology Bulletin    2013, 0 (6): 12-19.   Abstract （384）      PDF（pc） （1634KB）（3787）       Knowledge map    Save The calcium ion(Ca2+)is known as a key second messenger in plants, intracellular Ca2+ signals are relayed to downstream(transcription factors, NADPH oxidases genes)via different calcium sensor proteins(CaMs, CaMLs, CBLs, CDPKs), which further causes expression of related genes and responses to abiotic and biotic stresses. Calcium-dependent protein kinases(CDPKs)which are Ser/The protein kinases found in plants and some protozoa, play crucial roles in Ca2+-mediated signaling pathways. CDPKs are encoded by multigene families and are divided into four subgroups. CDPKs exhibit overlapping and distinct expression patterns, sub-cellular localizations, substrate specificities and redundancy and/or diversity functions. Here we review the recent advances on the structural characteristics, expression patterns, localizations, regulations substrates both in vivo and in vitro, inhibitors and functions in response to abiotic and biotic stresses of CDPKs in order to shed light on the functions and regulatory mechanisms. Reference | Related Articles | Metrics

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Genetic Analysis of FBA Trait in Upland Cotton with Major Gene Plus Polygenes Mixed Genetic Model MA Qi, LI Ji-lian, XU Shou-zhen, CHEN Hong, LIU Wen-hao, NING Xinzhu, LIN Hai Biotechnology Bulletin    2022, 38 (10): 148-158.   DOI: 10.13560/j.cnki.biotech.bull.1985.2021-1606 Abstract （415）   HTML （6）    PDF（pc） （3670KB）（3713）       Knowledge map    Save Cotton plant architecture is closely related to cultivation mode，mechanical collection efficiency and yield. Fruit branch angle（FBA）is one of the key factors that determine cotton plant architecture. Exploring the genetic characteristics on FBA of upland cotton（Gossyp-ium hirsutum）will provide important guidance for genetic breeding of cotton plant architecture. In this study，a natural population composed of 418 accessions of upland cotton from different regions，were used as the research object. Then，measuring the FBA with digital display protractor，we performed the variability and correlation analysis of FBA in different positions of cotton main stem，as well as the phenotypic description and statistical analysis of FBA of natural populations in different ecological environments. Simultaneously，the combined populations four generations（P1，P2，F1 and F2）constructed by parents with extremely different fruit branch angles were selected as the research objects，the multi-generation joint genetic analysis of FBA phenotypic traits of the four generations was carried out，and the genetic effect and heritability of major genes were estimated using the mixed genetic model of major gene plus polygenes of plant quantitative traits. Phenotypic identification analysis showed that the coefficient of variation for FBA in the natural population of upland cotton was relatively small，and the average coefficient of variation under the four environments was 5.63%. Furthermore，the middle fruit branch（the 4th-6th position from the base）angles best represented the level of FBA of the whole cotton plant. Additionally，mixed genetic model for major gene plus polygene demonstrated that the optimal model for controlling FBA was two major genes with equal additive effect，the additive effect value of major gene was 3.65，and the heritability was 90.22%. These results revealed that the FBA of upland cotton was mainly controlled by the main gene，and the heritability of the main gene was high. The above results are conducive to clarifying the genetic law of FBA of upland cotton，which is of important practical significance for molecular genetic analysis of FBA and plant architecture genetic breeding of upland cotton. Table and Figures | Reference | Related Articles | Metrics

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Genome Wide Association Study: Opportunities and Challenges in Genomic Research Zhang Yanming, Xing Guofang, Liu Meitao, Liu Xiaodong, Han Yuanhuai Biotechnology Bulletin    2013, 0 (6): 1-6.   Abstract （822）      PDF（pc） （1355KB）（3560）       Knowledge map    Save Genome wide association study(GWAS)is about screening for high-density molecular markers in certain populations in the range of the whole genome, then analyzing the correlations between the data of the molecular markers and the phenotypic traits. GWAS opened up a new chapter in genomic and genetic research, enabling linking genomics and genetics in unprecedented scale. GWAS is mainly applied in the analysis of complex traits of human diseases, leading to the identification of a number of genetic variants related to complex diseases and quantitative traits in human, hence it is to become one of the key approaches for human genomics. Application of GWAS in plant genomics just began, yet it has shown great advantages. It is becoming a research trend in plant genomics to use GWAS to discover genes related to complex quantitative traits to guide breeding programs. However, there exist some problems in GWAS, which are not as simple as expected. This review summarizes the current knowledge on GWAS with special emphasis on its applications in human and plant genomes and highlights its potential areas for future research. Reference | Related Articles | Metrics

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Gene Editing Tools Mediated by CRISPR-Cas Zuo Qisheng, Li Dong, Zhang Ya’ni, Li Bichun Biotechnology Bulletin    2014, 30 (7): 37-43.   Abstract （343）      PDF（pc） （1655KB）（3447）       Knowledge map    Save Recently, with the deepening of the research and transformation of CRISPR-Cas system, it has been applied as a new gene targeting modification technology, which can be directed to silence target genes. At present, this technology has been successfully used in HEK293, mouse and zebrafish cells and generated stable gene knockout cell lines, meanwhile gene knockout models has been constructed on the models animals such as mice and fruit flies. With the advantages of simple design, short time-consuming and higher efficiency, CRISPR-Cas has become a gene targeting knockout technology with broad application prospect. In this paper, structure and function mechanism of the CRISPR-Cas, also the current applications of Cas9/gRNA were reviewed. Reference | Related Articles | Metrics

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Research Progress in Biosynthesis of Secondary Metabolites of Microorganisms ZHAO Jiang-hua, FANG Huan, ZHANG Da-wei Biotechnology Bulletin    2020, 36 (11): 141-147.   DOI: 10.13560/j.cnki.biotech.bull.1985.2020-0475 Abstract （1018）   HTML （51）    PDF（pc） （1082KB）（3296）       Knowledge map    Save Microorganisms can produce a variety of natural secondary metabolites with biological activity,which have broad application prospects in medical,agricultural and food fields. The development of molecular biology, bioinformatics and sequencing technology has laid a theoretical foundation for the mining of secondary metabolites and provided operational tools. In recent years,there are more and more studies on the secondary metabolites of microorganisms. It is difficult to get satisfactory results by traditional methods. It will be a new direction to operate microorganisms at the molecular level,such as the regulation of secondary metabolite biosynthesis gene cluster.The strategies of finding new secondary metabolites and increasing the production of secondary metabolites were reviewed,by using the methods of heterologous expression of secondary metabolite biosynthesis gene cluster,ribosome engineering,epigenetic regulation,metabolic regulation and regulation of transcription and translation level,the aim is to excavate new secondary metabolites,improve the production of secondary metabolites,and provide a breakthrough for the exploration of new drugs. Reference | Related Articles | Metrics

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Pan-genome Sequencing and Comparative Genomic Analysis of Atrazine-degrading Bacteria WANG Ya-li, ZHU Shan-shan, YANG Feng-shan, MA Yu-kun, FU Hai-yan, LIU Chun-guang Biotechnology Bulletin    2019, 35 (7): 90-99.   DOI: 10.13560/j.cnki.biotech.bull.1985.2019-0360 Abstract （600）   HTML    PDF（pc） （3477KB）（3266）       Knowledge map    Save Arthrobacter aurescens TC1 and Pseudomonas sp. ADP are currently the model strains of atrazine-degrading bacteria. Microbacterium sp. HBT4 was screened in this experiment for discovering the similarities and differences of biological information among the 3 bacterial genomes and predicting important genes. In this study,pan-genome sequencing was carried out by using small DNA library preparation and sequencing technology on Illumina Hiseq 4000 sequencing platform. Genome composition analysis,gene function annotation,gene mutation detection and comparative genomics analysis were carried out by using related software. The nucleotide composition,collinearity and variation differences between the isolated Microbacterium HBT4 and model strains were analyzed. The genome size of the strain was about 3.53 Mb. It was predicted that there were 3 397 coding genes,1.33% repetitive sequences and 63 non-coding RNA in the strain HBT4. There were 3 324 annotations of gene function in general database and 1 149 annotations of gene function in special database. SNP,Small InDel and horizontal transfer genes were found through analyzing variance among strains and no structural variation genes were found. The number and proportion of GO-annotated genes in the specific genes of the strain in cell components,molecular functions and biological processes were obtained. From KEGG metabolic pathway enrichment map,it was found that the dihydrothiosyllysine residue succinyltransferase encoded by the specific gene was located between the metabolic pathway of α-ketoglutaric acid and succinyl coenzyme A in the tricarboxylic acid cycle. The proportional distribution,phylogenetic tree and collinearity of the core genome and non-essential genome of the three strains were obtained. It was found that there were 986 common gene families among the three strains,and 1171 specific gene families strain HBT4. Compared with the two model strains,the strain HBT4 obtained in this study had both similarities and differences in gene family. Reference | Related Articles | Metrics

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Advances in Metabonomics and Its Applications Zhou QiuxiangYu Xiaobin, Tu Guoquan, Wang Qiang, Hu Wenjun, Li Hanguang1, 3 Biotechnology Bulletin    2013, 0 (1): 49-55.   Abstract （462）      PDF（pc） （1232KB）（3231）       Knowledge map    Save Metabonomics(or metabolomics)is an essential part of systems biology. Through the study of metabolic changes inorganisms, their physiological and biochemical status can be known and the internal principle can be discovered. The meaning and research taskof metabonomics were introduced in this paper. Generation, technological platform of metabonomics and its application in plants, microorganisms,disease diagnosis and toxicology were summarized. A review on the development trend and challenges were also given. Reference | Related Articles | Metrics

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Research Progress of Acidobacteria Ecology in Soils WANG Guang-hua, LIU Jun-jie, YU Zhen-hua, WANG Xin-zhen, JIN Jian, LIU Xiao-bing Biotechnology Bulletin    2016, 32 (2): 14-20.   DOI: 10.13560/j.cnki.biotech.bull.1985.2016.02.002 Abstract （864）   HTML    PDF（pc） （1023KB）（3127）       Knowledge map    Save The phylum Acidobacteria is one of the most important bacterial groups in soils.Based on analysis of 16S rRNA gene sequences, the number of Acidobacteria generally represents about 20% of total soil bacterial communities, some of them even account for more than 50%, which suggests that Acidobacteria play important roles in soil ecological process.In this paper, we reviewed the research progress of Acidobacteria ecology in soils from several aspects, such as effects of plant types, altitude, nitrogen fertilizer and CO2 enrichment on the distribution of Acidobacteria, as well as rhizosphere effects of Acidobacteria, etc.The relationships between distributions of different taxonomic level Acidobacteria and environmental factors were summarized.The potential ecological functions of Acidobacteria were also presented in this paper.In the end of this paper, the importance for future study of Acidobacteria, such as reinforcement of isolation and pure culture, refinement of molecular ecological research, and adoption of metagenomics and single-cell sequencing approaches were also addressed. Reference | Related Articles | Metrics

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Advances of Metatranscriptomics Technology Ma Shu, Liu Huhu, Tian Yun, Lu Xiangyang Biotechnology Bulletin    2012, 0 (12): 46-50.   Abstract （485）      PDF（pc） （1362KB）（3115）       Knowledge map    Save Metatranscriptomics is a new subject to study the transcription situation and regulation rules of entire genomes of colonialorganism under a certain specific environment and period in the overall level. The emergence, research strategies and current applications of theMetatranscriptomic were reviewed briefly and the application prospect of it was also anticipated in this paper. Reference | Related Articles | Metrics

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The Research of an Improved Method for Protein Staining in Electrophoresis with Coomassie Brilliant Blue Rao Weiqiao, Xiao Weimin, Zhang Jiyuan, Rao Yuan, Zi Jin Biotechnology Bulletin    2014, 30 (9): 58-64.   Abstract （329）      PDF（pc） （1511KB）（3048）       Knowledge map    Save In order to establish a convenient, sensitive, economic and Mass Spectrometry compatible staining method for protein polyacrylamide gel electrophoresis, herein, we established optimized staining methods based on Blue Silver Staining, by optimizing the gel immobilization time and buffer content. Comparison result indicated the method 1 keep the similar high sensitivity and well Mass Spectrometry compatible feature as Blue Silver Staining, what’ s more, is more easily conducted, less organic solvent consuming and have better correlation between protein amount and staining density. Reference | Related Articles | Metrics

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Research Progress on Optimizing the Expression of Exogenous Proteins in Escherichia coli SU Peng, GONG Guo-li Biotechnology Bulletin    2017, 33 (2): 16-23.   DOI: 10.13560/j.cnki.biotech.bull.1985.2017.02.003 Abstract （369）   HTML    PDF（pc） （982KB）（3006）       Knowledge map    Save Escherichia coli expression system has become the first choice of expression of exogenous protein,the expression system for recombinant protein presents many advantages,and however,sometimes expressed exogenous protein is easily attacked by host protease or cannot form insoluble inclusion bodies because of incorrect folding,thus by which the expression is limited. This paper summarizes some optimization strategies used in the process of the expression of exogenous proteins in E. coli,mainly including the expression of rare codons,application of fusion tags,change of E. coli strains for expression,and reducing the formation of inclusion bodies,a single protein production system,auto-induction,fed-batch cultivation,high cell-density culture,etc.,which aims at exploring the optimal strategy for the expression of exogenous protein. Reference | Related Articles | Metrics

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Review on the Application of Integrated Transcriptome and Proteome Analysis in Biology JIANG Ke-ren, MA Zheng, ZHENG Hang, LIU Xiao-Jun Biotechnology Bulletin    2018, 34 (12): 50-55.   DOI: 10.13560/j.cnki.biotech.bull.1985.2017-0929 Abstract （539）   HTML    PDF（pc） （1223KB）（2977）       Knowledge map    Save The main steps of gene expression in an organism include transcription and protein synthesis,mRNA is an intermediate product of gene expression,and protein is the executive of gene function. For understanding the specific regulatory process of gene expression,the mRNA and protein should be synchronously monitored,then transcriptome and proteome data are compared,and thus both may be linked while an integrated analysis is conducted at the transcriptional and protein levels. In theory,the correlation between transcriptome and proteome data obtained from the same growth conditions and the cells,tissues or organs of the same state should be high;however,a lot of literature reported the irrelevant or negative correlations of expression trend between transcriptome and proteome data. Here we reviewed the principles and features of transcriptome and proteome sequencing and the advantages of transcriptome and proteome integrated analysis,as well as its applications in animal,plant and microbial. Meanwhile,we analyzed the reasons that might cause inconsistence of gene expression trends between transcriptional and protein levels,providing a reference for future study on molecular biology and multi-omics analysis. Reference | Related Articles | Metrics

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