Biotechnology Bulletin ›› 2017, Vol. 33 ›› Issue (10): 143-147.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0536

• Research report • Previous Articles     Next Articles

Isolation,Identification and Degrading Properties of Phlorizin-Degrading Fungi

LIU Shu-yan1, WANG Fang2, WANG Jian-yu2, LIN Rong-shan2   

  1. 1. Liaoning Entry-Exit Inspection and Quarantine Bureau,Dalian 116015
    2. Shan Dong Agricultural University,Taian 271018
  • Received:2017-06-29 Online:2017-10-29 Published:2017-10-29
  • Contact: 林榕姗,女,博士,副教授,研究方向:资源与环境微生物;E-mail:lrs2680@163.com

Abstract: Screening highly efficient phlorizin-degrading microorganism is important for bioremediation of phlorizin-contaminated environment. This study aimed to isolate and identify high efficient phlorizin-degrading microorganism from the rhizosphere soils of apple,then to characterize degrading properties of target strains. Phlorizin-degrading microorganism were screened by the plate-culture method,used phloridzin as the sole carbon sources,and then the target strains were identified by the morphological and molecular methods. Degrading properties of phlorizin -degrading microorganism were determined under different condition of culture. The results showed that a phlorizin-degrading fungal strain AMCC300100 identified as Aspergillus terreus were obtained from the rhizosphere soils of apple replantd and non-replanted orchards surrounding Bohai gulf area. The phlorizin degradation rate of the strain AMCC300100 has reached 88.96%(phlorizin initial concentration of 2 mmol/L,pH5.0,2% inoculum)cultured 96h under the conditions of 40℃,160r/min;The strain AMCC300110 have high-efficient degradation ability not only for phlorizim but also for the other phenolic acids. Though plate-culture method,We obtained a fungal strain identified as Aspergillus terreus,this fungal strain have high-efficient degradation ability for phlorizim,and have tremendous potential in solving apple replant disease.

Key words: phlorizin, biodegradation, replant disease, Aspergillus terreus