Isolation，Identification and Degrading Properties of Phlorizin-Degrading Fungi

doi:10.13560/j.cnki.biotech.bull.1985.2017-0536

Abstract

Abstract: Screening highly efficient phlorizin-degrading microorganism is important for bioremediation of phlorizin-contaminated environment. This study aimed to isolate and identify high efficient phlorizin-degrading microorganism from the rhizosphere soils of apple，then to characterize degrading properties of target strains. Phlorizin-degrading microorganism were screened by the plate-culture method，used phloridzin as the sole carbon sources，and then the target strains were identified by the morphological and molecular methods. Degrading properties of phlorizin -degrading microorganism were determined under different condition of culture. The results showed that a phlorizin-degrading fungal strain AMCC300100 identified as Aspergillus terreus were obtained from the rhizosphere soils of apple replantd and non-replanted orchards surrounding Bohai gulf area. The phlorizin degradation rate of the strain AMCC300100 has reached 88.96%（phlorizin initial concentration of 2 mmol/L，pH5.0，2% inoculum）cultured 96h under the conditions of 40℃，160r/min；The strain AMCC300110 have high-efficient degradation ability not only for phlorizim but also for the other phenolic acids. Though plate-culture method，We obtained a fungal strain identified as Aspergillus terreus，this fungal strain have high-efficient degradation ability for phlorizim，and have tremendous potential in solving apple replant disease.

Key words: phlorizin, biodegradation, replant disease, Aspergillus terreus

LIU Shu-yan, WANG Fang, WANG Jian-yu, LIN Rong-shan. Isolation，Identification and Degrading Properties of Phlorizin-Degrading Fungi[J]. Biotechnology Bulletin, 2017, 33(10): 143-147.

References

[1]孙海滨, 毛志泉, 朱树华. 环渤海湾地区连作苹果园土壤中酚酸类物质变化[J]. 生态学报, 2011, 31(1)：90-97.
[2] 黄春生, 熊明. 连作障碍产生的原因及改善途径[J]. 上海蔬菜, 2010, 5：62-64.
[3]王闯, 徐公义, 葛长城, 等. 酚酸类物质和植物连作障碍的研究进展[J]. 北方园艺, 2009, 3：134-137.
[4]张江红, 毛志泉, 王丽琴, 等. 根皮苷对平邑甜茶幼苗生理特性的影响[J]. 中国农业科学, 2007, 40(3)：492-498.
[5]张兆波, 毛志泉, 朱树华. 6种酚酸类物质对平邑甜茶幼苗根系线粒体及抗氧化酶活性的影响[J]. 中国农业科学, 2011, 44(15)：3177-3184.
[6]张一, 王凤忠, 张铭铄, 等. 苹果园土壤中一株根皮苷降解菌的筛选[J]. 山东农业科学, 2011, 12：59-63.
[7]王倩, 李晓林. 苯甲酸和肉桂酸对西瓜幼苗生长及枯萎病发生的作用[J]. 中国农业大学学报, 2003, 8(1)：83-86.
[8]崔晓敬. 苹果砧木抗自毒作用突变体筛选及其抗性生理机制研究[D]. 保定：河北农业大学, 2010.
[9]Zhang Y, Gu M, Shi K, et al. Effects of aqueous root extracts and hydrophobic root exudates of cucumber(Cucumis sativus L. )on nuclei DNA content and expression of cell cycle-related genes in cucumber radicles[J]. Plant Soil, 2011, 327：455-463.
[10]Seal AY, Pratley JE, Haig T, et al. Identification and quantitation of compounds in a series of allelopathic and non-allelopathic rice root exudates[J]. Journal of chemical Ecology, 2004, 30：1647-1662.
[11] Manici LM, Ciavatta C, Kelderer M. Erschbaumer G. Replant problems in South Tyrol：role of fungal pathogens and microbial population in conventional and organic apple orchards[J]. Plant Soil, 2003, 256：315-324.
[12]魏景超. 真菌鉴定手册[M]. 上海：上海科技出版社, 1997.
[13]Cui XJ, Wang YA, Zhen WC. The effects of phlorizin stress on the protective enzyme and metabolic regulation substances in the root of M. micromalus[J]. Frontiers of Agriculture in China, 2010, 3：321-324.
[14]Jennings AJ, Nelson CJ. Zone of autotoxic influence around established Alfalfa plants[J]. Agronomy Journal, 2002, 94(5)：1104-1111.
[15]白茹. 苹果连作障碍中自毒物质的研究[D]. 杨凌：西北农林科技大学, 2009.
[16]Cao L, Liu H, Zhang H, et al. Characterization of a newly isolated highly effective 3, 5, 6-trichloro-2-pyridinol degrading strain Cupriavidus pauculus P2[J]. Curr. Microbiol. , 2012, 65：231-236.
[17]黄兴如, 张彩文, 张瑞杰, 等. 多环芳烃降解菌的筛选、鉴定及降解特性[J]. 微生物学通报, 2016, 43(5)：965-973.