关键词: 长春花, 长春质碱和文朵灵, 茉莉酸, 茉莉酸异亮氨酸共轭物合酶, 次生代谢调控

Abstract: This work aims to reveal JAR1 gene regulation roles in the synthesis of catharanthine and vindoline via cloning it from Catharanthus roseus and its transient overexpression in the leaves of C. roseus. The JAR1 gene was cloned using total cDNA as template,and the recombinant plasmid pBIGD-JAR1 was constructed. Transiently transformed to the leaves of C. roseus mediated by Agrobacterium,HPLC and semi-quantitative RT-PCR were utilized to analyze the effects of JAR1 overexpression on the synthesis of catharanthine and vindoline. The results showed that gene JAR1 of 1700 bp was cloned successfully from the leaves of C. roseus,and the transcription of gene JAR1 in the leaves of C. roseus transiently transformed with pBIGD-JAR1was higher than that in the control. The overexpression of JAR1 significantly induced the expressions of key pathway genes（CrTDC,CrG10H,CrDL7H,CrSTR,and CrLAMT）as well as the accumulation of catharanthine and vindoline. The above results suggest that overexpression of JAR1 induce the expressions of key metabolic enzyme genes by promoting the jasmonic acid signal transduction pathway,and subsequently promote the accumulation of catharanthine and vindoline.

Key words: Catharanthus roseus, catharanthine and vindoline, jasmonic acid, JAR1, regulation of secondary metabolism