八株海洋微藻Nannochloris sp.生长及生化性状评价

doi:10.13560/j.cnki.biotech.bull.1985.2016.06.034

生物技术通报 ›› 2016, Vol. 32 ›› Issue (6): 231-237.doi: 10.13560/j.cnki.biotech.bull.1985.2016.06.034

八株海洋微藻Nannochloris sp.生长及生化性状评价

陈程浩^1,2, 吴嘉仪³, 汤明星^1,2, 李涛¹, 吴华莲¹, 王广华¹, 戴世鲲¹, 向文洲¹

1. 中国科学院南海海洋研究所中国科学院热带海洋生物资源与生态重点实验室，广州 510301
2. 中国科学院大学，北京 100049
3. 广州市海怡康生物科技公司，广州 510301)

收稿日期:2015-10-10 出版日期:2016-06-27 发布日期:2016-06-28
作者简介:陈程浩，男，硕士研究生，研究方向：微藻生物技术；E-mail：cch90@126.com
基金资助:
海洋公益性行业科研专项项目(201305018-3)，海南省科技兴海专项项目(2015XH04)，广省海洋渔业科技与产业发展专项项目(A201501C09)

Evaluation on Growth and Biochemical Properties of Eight Strains of Marine Microalgae Nannochloris sp.

CHEN Cheng-hao^1，2, WU Jia-yi³, TANG Ming-xing^1，2, LI Tao¹, WU Hua-lian¹, WANG Guang-hua¹, DAI Shi-kun¹, XIANG Wen-zhou¹

1. Key Laboratory of Tropical Marine Bio-resources and Ecology，South China Sea Institute of Oceanology，Chinese Academy of Sciences，Guangzhou 510301
2 University of Chinese Academy of Sciences，Beijing 100049
3 Guangzhou Haiyikang Bio-tech Company，Guangzhou 510301

Received:2015-10-10 Published:2016-06-27 Online:2016-06-28

摘要/Abstract

摘要： 通过对从南海海域筛选分离纯化的8株Nannochloris sp.海洋微藻进行生长和生化特性分析，评估该属微藻的生产应用潜力。采用干重法、索氏提取法、凯氏定氮法、苯酚硫酸法、气相色谱质谱联用分别测定微藻生长以及油脂、蛋白质、多糖含量及脂肪酸组成。结果表明，8株微藻的最大生物量干重为0.39-0.91 g/L，生长最快的为SCS-761藻株；总脂含量为(19.06-33.67)%，其中SCS-249、SCS-589、SCS-655藻株总脂含量超过25%，且SCS-655藻株油脂产率高达22.23 ± 0.71 mg/(L·d)；SCS-249、SCS-325、SCS-355、SCS-640、SCS-769藻株蛋白质含量超过30%，其中SCS-640藻株蛋白质含量最高(36.70 ± 2.20)%；而各株藻的多糖含量介于19.23%-29.34%，其中SCS-761最高，SCS-655最低。另外，脂肪酸组成分析结果显示8株微藻的脂肪酸组成结构相似，主要为C16饱和脂肪酸和C18系脂肪酸。不饱和脂肪酸以亚油酸相对含量最高，SCS-249、SCS-355、SCS-761藻株亚油酸相对含量超过40%。海洋微藻Nannochloris生物活性物质资源丰富，含有较高的脂质、蛋白质和亚油酸含量，应用潜力可观，应对该属微藻进行更为深入的研究开发，为该属微藻高值化综合性开发奠定基础。

关键词: 海洋微藻, 生长, 油脂, 蛋白质, 多糖, 脂肪酸

Abstract: This work is to evaluate the productive potential of Nannochloris sp. by analyzing the growth and biochemical characteristics of 8 strains screened from the South China Sea region. Using dry weight determination，soxhlet extraction，kjeldahl nitrogen method，phenol sulfuric acid determination combined with gas chromatography-mass spectrometry technology，the microalgal growth，the content of lipid，protein and polysaccharide and the composition of fatty acids were analyzed correspondingly. The maximum dry weight of eight strains was 0.39 - 0.91 g/L and the SCS-761 strain grew the fastest. The total lipid content ranged in 19.06% - 33.67%，and that of SCS-249，SCS-589，and SCS-655 was over 25%，and the lipid productivity of SCS-655 strain was as high as 22.23 ± 0.71 mg/(L·d). The protein contents of SCS-249，SCS-325，SCS-355，SCS-640，and SCS-769 were over 30%，of which the highest for SCS-640 strain(36.70 ± 2.20)%. Moreover，the polysaccharide content of each strain was between 19.23% to 29.34%，of which the highest was SCS-761 strain and the least was SCS-655 strain. In addition，the analysis of fatty acid composition showed that the structures of fatty acid in all microalgae strains were similar，mainly composed of C16 saturated fatty acids and C18 series of fatty acids. Linoleic acid was the highest content among unsaturated fatty acids，and the linoleic acid contents of SCS-249，SCS-355，and SCS-761 were over 40%. In conclusion，the results indicates that marine microalgae Nannochloris sp. has relatively abundant germplasm resources including lipid，protein and linoleic acid，and owns considerable application potential；thus it is necessary to have more in-depth research.

Key words: marine microalgae, growth, lipid, protein, polysaccharide, fatty acid

陈程浩, 吴嘉仪, 汤明星, 李涛, 吴华莲, 王广华, 戴世鲲, 向文洲. 八株海洋微藻Nannochloris sp.生长及生化性状评价[J]. 生物技术通报, 2016, 32(6): 231-237.

CHEN Cheng-hao, WU Jia-yi, TANG Ming-xing, LI Tao, WU Hua-lian, WANG Guang-hua, DAI Shi-kun, XIANG Wen-zhou. Evaluation on Growth and Biochemical Properties of Eight Strains of Marine Microalgae Nannochloris sp.[J]. Biotechnology Bulletin, 2016, 32(6): 231-237.

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八株海洋微藻Nannochloris sp.生长及生化性状评价

Evaluation on Growth and Biochemical Properties of Eight Strains of Marine Microalgae Nannochloris sp.

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