植物4香豆酸：辅酶A连接酶研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2017.04.003

摘要/Abstract

摘要： 4香豆酸：辅酶A连接酶（4-coumarate：coenzyme A ligase EC6.2.1.12,4CL）是木质素生物合成途径中的一个关键酶,催化肉桂酸及其衍生物生成相应的硫酯。同时也是苯丙烷类代谢途径中的第三个步骤,连接木质素前体和各个分支途径的纽带,在木质素合成过程中发挥了重要的调控作用。近年来利用基因工程手段调控木质素生物合成,降低木质素含量,减少制浆造纸过程中的污染已成为研究热点。结合国内外关于4CL的研究成果,对植物4CL基因家族、酶学性质、晶体结构以及4CL在调控木质素生物合成中的作用等方面进行综述,并对4CL的研究方向提出展望,旨为对该基因的研究提供参考。

关键词: 4CL, 木质素, 酶学, 晶体学, 基因调控

Abstract: 4CL（4-coumarate：coenzyme A ligase,EC6.2.1.12）is a key enzyme in the lignin biosynthesis pathway,and it catalyzes a hydroxycinnamic acids and its derivatives to generate the corresponding thioester. Concurrently,4 CL is also the third step in the metabolic pathway of phenylpropane,ligating the precursor of lignin and varied branch pathways,playing the critical regulating role in the lignin synthesis. It has become a research hotspot that uses the genetic engineering way to alter lignin biosynthesis,to reduce lignin content,and to decrease the pollution in pulp and papermaking process. Combining the new reports about 4CL in recent years,we summarized the research progresses on phylogenetics,enzymatic activities,crystal structure and catalytic mechanism and regulation of 4CL in lignin biosynthesis. The future directions of researches on 4CL were also suggested, This paper provides reference information for 4CL in the future study.

Key words: 4CL, lignin, enzymology, crystallography, gene regulation

田晓明, 颜立红, 向光锋, 蒋利媛. 植物4香豆酸：辅酶A连接酶研究进展[J]. 生物技术通报, 2017, 33(4): 19-26.

TIAN Xiao-ming, YAN Li-hong, XIANG Guang-feng, JIANG Li-yuan. Research Progress on 4-Coumarate：Coenzyme A Ligase（4CL）in Plants[J]. Biotechnology Bulletin, 2017, 33(4): 19-26.

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