一氧化氮（NO）对镉胁迫下小麦幼苗氧化损伤的影响

doi:10.13560/j.cnki.biotech.bull.1985.2017.05.015

摘要/Abstract

摘要： 为探讨一氧化氮（NO）对重金属镉胁迫后小麦幼苗氧化损伤的影响。采用营养液水培法,以“晋麦8号”为材料,一氧化氮供体硝普钠（SNP）,NO清除剂牛血红蛋白（Hb）及SNP类似物亚铁氰化钠（SF）分别处理小麦幼苗,研究NO在镉（Cd）胁迫下对小麦幼苗抗氧化系统的影响。结果显示,SNP处理可以缓解镉胁迫对幼苗生长抑制,显著增加可溶性蛋白、叶绿素和GSH含量,减少丙二醛（MDA）和过氧化氢（H₂O₂）的含量,并降低超氧阴离子（O₂·^-）产生速率、可溶性糖和脯氨酸的积累。而NO清除剂牛血红蛋白（Hb）处理使镉胁迫对小麦幼苗的毒害增强,SNP类似物亚铁氰化钠（SF）处理则没有缓解效应;进一步实验发现,SNP降低了镉胁迫下超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽还原酶（GR）和抗坏血酸过氧化物酶（APX）等抗氧化相关酶的活性,减轻了镉胁迫的氧化损伤。NO可以通过调节抗氧化酶系统来缓解Cd对植物的毒害。

关键词: 小麦幼苗, 镉胁迫, 一氧化氮, 氧化损伤

Abstract: This study is to further study the response mechanism of nitric oxide gas signal on oxidative damage of wheat seedlings under cadmium（Cd）stress. Through nutrient solution cultivation,Triticum aestivum ‘Jinmai 8’ was selected as the plant material,and nitric oxide（NO）-donor sodium nitroprusside（SNP）,NO-scavenger haemoglobin（Hb）,and sodium ferrocyanide（SNP analogue）were used to investigate the effects of NO on oxidative damage metabolism in seedlings under Cd stress. The results showed that SNP alleviated the growth inhibition in wheat seedlings,significantly increased the contents of soluble protein,chlorophyll and glutathion,decreased the contents of malondiadehyde（MDA）and hydrogen peroxide（H₂O₂）,as well as the production of O₂·^-,and the accumulations of soluble sugar and proline. However,the NO-scavenger hemoglobin treatment increased the toxicity of Cd stress to wheat seedlings,and the SNP analogue treatment presented no effect. Meanwhile,SNP decreased the activities of superoxide dismutase（SOD）,catalase（CAT）,glutathione reductase（GR）and ascorbate peroxidase（APX）,which eliminated the oxidative damage under Cd stress. Conclusively,NO alleviates Cd-damage by regulating the antioxidase system.

Key words: wheat seedlings, cadmium stress, nitric oxide, oxidative damage

马晓丽, 冀瑞萍, 田保华, 霍建新. 一氧化氮（NO）对镉胁迫下小麦幼苗氧化损伤的影响[J]. 生物技术通报, 2017, 33(5): 102-107.

MA Xiao-li, JI Rui-ping, TIAN Bao-hua, HUO Jian-xin. Effects of Nitric Oxide on Oxidative Damage Metabolism in Wheat Seedling Under Cadmium Stress[J]. Biotechnology Bulletin, 2017, 33(5): 102-107.

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