一氧化氮对干旱胁迫下白菜幼苗微管蛋白的影响

doi:10.13560/j.cnki.biotech.bull.1985.2016.02.023

摘要/Abstract

摘要： 旨在探讨一氧化氮对干旱胁迫后白菜叶片微管蛋白的影响，以津育11白菜种子为实验材料，分别采用15、18、21、24和27 g/mL 5种不同浓度的PEG6000溶液对7日龄白菜幼苗进行模拟干旱处理，通过紫外分光光度计和SDS-PAGE聚丙烯酰胺凝胶电泳研究不同处理组白菜幼苗叶片微管蛋白含量的差异，确定PEG6000最佳处理浓度为27 g/mL。50、100和150 μmol/L 3个不同浓度的一氧化氮对PEG6000处理组进行熏蒸，观察不同处理组白菜幼苗叶片微管蛋白含量的变化差异。结果显示，与对照组相比不同浓度PEG6000对白菜幼苗叶片微管蛋白含量都呈增加的趋势，经一氧化氮处理后降低；且在干旱胁迫的过程中白菜体内编码一氧化氮的基因表达量和一氧化氮含量都有大幅上调。因此，生理浓度的一氧化氮对干旱引起的微管蛋白的解聚有一定的缓解作用，减少胁迫对植株造成的损害。

关键词: 白菜, 一氧化氮, 干旱, 微管蛋白

Abstract: In order to study the effect of nitricoxide(NO)on microtubulin in drought-stressed Brassica pekinensis, using B.pekinensis cultivar Jinyu-11 as experimental material, and the drought situation was simulated by treating 7-day seedlings with 15, 18, 21, 24, 27 g/mL of PEG6000 concentrations.Ultraviolet spectrophotometry and SDS-PAGE were used to investigate the differences of microtubulin content in cabbage seedling leaves, and the optimal treatment concentration of PEG6000 was determined to be 27 g/mL.Then the pretreated seedlings were fumigated with various concentrations of NO：50, 100, and 150 μmol/L respectively, and the variations of microtubulin content in the treated cabbages were observed.Results showed that the contents of microtubulin in cabbage seedling leaves demonstrated the trend of increasing with PEG6000 pre-treatments compared to control, but decreased after NO treatment.Moreover, both the content of endogenous NO and the expression of NO-related genes increased significantly under drought stress.Therefore, physiological concentration of NO alleviated the disaggregation of microtubulin caused from drought stress at certain level, which eliminated the damages to plants from the stress.

Key words: Brassica pekinensis, nitric oxide, drought stress, microtubulin

马晓丽, 冀瑞萍. 一氧化氮对干旱胁迫下白菜幼苗微管蛋白的影响[J]. 生物技术通报, 2016, 32(2): 172-177.

MA Xiao-li, JI Rui-ping. The Influence of Exogenous Nitric Oxide on Microtubulin of Brassica pekinensis Under Drought Stress[J]. Biotechnology Bulletin, 2016, 32(2): 172-177.

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