一株黄色杆菌的分离鉴定及对邻苯二甲酸酯的降解研究

doi:10.13560/j.cnki.biotech.bull.1985.2018-0393

生物技术通报 ›› 2018, Vol. 34 ›› Issue (10): 157-164.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0393

一株黄色杆菌的分离鉴定及对邻苯二甲酸酯的降解研究

王嘉翼, 樊双虎, 任超, 王俊欢, 杨婷, 贾阳, 李先军, 闫艳春

中国农业科学院研究生院,北京 100081

收稿日期:2018-04-28 出版日期:2018-10-26 发布日期:2018-11-07
作者简介:王嘉翼,女,硕士研究生,研究方向：微生物分子生物学与基因工程;E-mail：15524115399@163.com
基金资助:
国家自然科学基金项目（31170119,31540067）,中国农业科学院基本科研业务费（0042014011,1610042017001）

Identification of Newly Isolated Xanthobacter sp. and Its Degradability to Phthalic Acid Esters

WANG Jia-yi FAN, Shuang-hu, REN Chao, WANG Jun-huan, YANG Ting, JIA Yang, LI Xian-jun, YAN Yan-chun

Graduate School of Chinese Academy of Agricultural Sciences,Beijing 100081

Received:2018-04-28 Published:2018-10-26 Online:2018-11-07

摘要/Abstract

摘要： 邻苯二甲酸酯（PAEs）是环境中普遍存在的有机污染物,具有环境雌激素效应,对人体健康和生态安全造成严重威胁。菌株YC-JY1分离自受石油长期污染的土壤中,可以利用邻苯二甲酸二丁酯（DBP）作为唯一的碳源进行生长;经16S rDNA 鉴定,确定其属于黄色杆菌属（Xanthobactersp.）。菌株YC-JY1降解DBP的最适条件为30℃,pH 7.0,无NaCl添加;在此条件下,100 mg/L DBP 在5 d内能被完全降解。随着DBP浓度的升高,菌株YC-JY1在5 d内对200 mg/L - 400 mg/L DBP的降解率在94%以上;通过底物谱实验发现,菌株YC-JY1对其它邻苯二甲酸酯具有广泛的利用能力,其中邻苯二甲酸二戊酯（DPeP）,邻苯二甲酸二己酯（DHP）的降解率均在90%以上;通过高效液相色谱-质谱联用确定DBP中间代谢产物为邻苯二甲酸单丁酯（MBP）,邻苯二甲酸（PA）。由此推断,在菌株YC-JY1的作用下,DBP的起始代谢途径为DBP首先水解为MBP,继而水解为PA。

关键词: 邻苯二甲酸二丁酯, 生物降解, 黄色杆菌YC-JY1, 代谢途径

Abstract: Phthalic acid esters（PAEs）are ubiquitous organic pollutants with environmental estrogen effects,which cause serious threats to human health and safety of ecological system. Strain YC-JY1 was isolated from long-time contaminated soil by petroleum,and grew by utilizing dibutyl phthalate（DBP）as the sole carbon source. After 16S rDNA sequencing,it was identified to be a strain of Xanthobacter genus. The optimal conditions for DBP degradation by this strain were 30℃,pH 7.0 and no NaCl added. Under this condition,100 mg/L DBP was completely degraded within 5 d. As the concentration of DBP increased,the degradation rate of it to DBP with initial concentration of 200 - 400 mg/L was over 94% after 5 d. Through substrate spectrum experiments,it was found that strain YC-JY1 utilized extensively other PAEs. The degradation rate of it to dipentyl phthalate（DPeP）and dihexyl phthalate（DHP）were all above 90%. The intermediates of DBP were determined to be mono-butyl phthalate（MBP）and phthalic acid（PA）by HPLC-MS. It was inferred that the initial metabolic process of DBP was as firstly hydrolyzed to MBP and then to PA by the strain YC-JY1.

Key words: Di-n-butyl phthalate, biodegradation, Xanthobacter sp. YC-JY1, metabolic pathway^?

王嘉翼, 樊双虎, 任超, 王俊欢, 杨婷, 贾阳, 李先军, 闫艳春. 一株黄色杆菌的分离鉴定及对邻苯二甲酸酯的降解研究[J]. 生物技术通报, 2018, 34(10): 157-164.

WANG Jia-yi FAN, Shuang-hu, REN Chao, WANG Jun-huan, YANG Ting, JIA Yang, LI Xian-jun, YAN Yan-chun. Identification of Newly Isolated Xanthobacter sp. and Its Degradability to Phthalic Acid Esters[J]. Biotechnology Bulletin, 2018, 34(10): 157-164.

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一株黄色杆菌的分离鉴定及对邻苯二甲酸酯的降解研究

Identification of Newly Isolated Xanthobacter sp. and Its Degradability to Phthalic Acid Esters

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