基于高分辨质谱的非靶向代谢组学在食品造假鉴定中的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2018-0632

摘要/Abstract

摘要： 食品造假属于以经济利益为驱动的食品欺诈（Economically motivated adulteration,EMA）,是目前影响及破坏食品安全秩序的重要因素之一。而随着人们生活水平的迅速提高,对食品品质提出了更高的要求,当下食品造假现象是公众极为关注的问题。以往,针对已知的目标物质进行定量检测的靶向代谢组学技术在食品检测中广泛应用,代谢组学技术已经成为食品科学研究中不可或缺的工具。近年来,针对食品造假现象,代谢组学的另一个分支,基于高分辨质谱（High-resolution mass spectrometry,HRMS）的非靶向代谢组学（Non-targeted metabolomics,NTM）开始运用到食品造假的鉴定中,并已取得快速的发展。对基于HRMS的非靶向代谢组学的食品造假鉴定工作流程进行了介绍,对其数据处理方式进行了概括,并就其在食品物种（品种）、产地和品质等造假鉴定相关应用分别进行了总结,最后还对基于高分辨质谱的非靶向代谢组学应用于食品检测中优点和缺点进行了概括,并对其在其他研究方向进行了展望,旨在对食品检测中代谢组学的最新研究进展进行总结和归纳。

关键词: 高分辨质谱, 代谢组学, 食品造假

Abstract: Food fraud is a type of economically motivated adulteration（EMA）,one of the most important factors affecting and destroying food safety. With the rapid development of living conditions,people demand food with better quality,thus food fraud has been concerned seriously by the public. Targeted metabolomics techniques for quantitative detection of known target substances have been widely used in food inspection,and metabolomics technology has become an indispensable tool in food science research. Regarding food fraud occurring in recent years,another branch of metabolomics,based on high resolution mass spectrometry（HRMS）,non-targeted metabolomics（NTM）was gradually applied and has made great development in the identification of food fraud. Here we introduced the workflow of identifying food fraud using HRMS-based NTM and summarized the data-progressing methods. Then we made a sum on the relevant applications for identifying frauds in food species/varieties,origin and quality respectively. Finally,we analyzed the advantages and disadvantages of HRMS-based NTM in food inspection and prospected other research directions. It is aimed that this work summarizes and describes the most recent metabolomics results in food inspection.

Key words: high-resolution mass spectrometry（HRMS）, metabolomics, food fraud

赖博文, 刘玢, 梁永康. 基于高分辨质谱的非靶向代谢组学在食品造假鉴定中的研究进展[J]. 生物技术通报, 2019, 35(2): 192-197.

LAI Bo-wen, LIU Bin, LIANG Yong-kang. Research Progress on Food Fraud Using Non-targeted Metabolomics Based on High-resolution Mass Spectrometry[J]. Biotechnology Bulletin, 2019, 35(2): 192-197.

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