桃红侧耳子实体颜色的遗传规律研究

doi:10.13560/j.cnki.biotech.bull.1985.2019-0127

生物技术通报 ›› 2019, Vol. 35 ›› Issue (5): 70-75.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0127

桃红侧耳子实体颜色的遗传规律研究

张泽华^1,2, 高巍², 邬向丽², 黄晨阳², 常明昌¹, 张金霞²

1.山西农业大学食品科学与工程学院,太谷 030801;
2.中国农业科学院农业资源与农业区划研究所农业农村部农业微生物资源收集与保藏重点实验室,北京 100081

收稿日期:2019-02-19 出版日期:2019-05-26 发布日期:2019-05-23
作者简介:张泽华,男,硕士研究生,研究方向：食用菌栽培与育种;E-mail：18235444757@163.com
基金资助:
国家食用菌产业技术体系（CARS20）,中国农业科学院基本科研业务费专项所级统筹项目（1610132018035）,山西省煤基重点科技攻关项目（FT2014-03-01）

Inheritance Study on Fruiting Body Color of Pleurotus djamor（Rumph. ex Fr.）Boedijn

ZHANG Ze-hua^1,2, GAO Wei², WU Xiang-li², HUANG Chen-yang², CHANG Ming-chang¹, ZHANG Jin-xia²

1.College of Food Engineering Shanxi Agricultural University,Taigu 030801;
2.Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences/Key Laboratory of Microbial Resources,Ministry of Agriculture and Rural Affairs,Beijing 100081

Received:2019-02-19 Published:2019-05-26 Online:2019-05-23

摘要/Abstract

摘要： 子实体颜色是食用菌的重要商品性状之一,也是食用菌遗传育种的重要方向。以桃红侧耳[Pleurotus djamor（Rumph. ex Fr.）Boedijn]的粉红色菌株CCMSSC00450和灰褐色菌株CCMSSC04445为亲本,通过原生质体单核化技术,分别获得各自的组成型单核菌株PDm52、PDm17和PDm99、PDm14。经相互配对杂交和出菇试验,4个杂交子均可结实,且色泽不同,其中PDm52×PDm99是粉红色、PDm52×PDm14是粉灰色、PDm17×PDm14是灰褐色、PDm17×PDm99是白色。以粉红色杂交子PDm52×PDm99的207个单孢单核体作为粉红色子实体的分离群体,以PDm17为测交菌株,构建测交群体。出菇结果显示,测交群体的子实体颜色出现明显的性状分离,由深到浅连续分布,存在粉红色、淡粉色、白色等。结果表明,P. djamor子实体的粉红色相对于白色为不完全显性,白色为隐性;子实体颜色性状是具有高遗传力的多基因控制的数量性状;菌盖颜色和菌褶颜色存在极显著相关性。

关键词: 桃红侧耳, 子实体颜色, 数量性状, 测交, 杂交

Abstract: The color of fruiting body is one of the most important commercial traits of edible mushrooms and an important objective for breeding superior mushroom cultivars. In this study,a pink strain CCMSSC00450 and a taupe strain CCMSSC04445 of Pleurotus djamor（Rumph. ex Fr.）Boedijn were used as dikaryotic parental lines,and the constitutive monokaryons of two strains i.e.,PDm52 and PDm17 for strain CCMSSC00450,PDm99 and PDm14 for CCMSSC04445,were obtained by protoplast monokaryonization. After the cross-mating of 4 constitutive monokaryons,4 hybrid dikaryons were generated and cultivated for phenotyping. The 4 hybrid strains showed different color,i.e.,pink for PDm52×PDm99,pinkish gray for PDm52×PDm14,taupe for PDm17×PDm14,and white for PDm17×PDm99. A total 207 single spore isolates of the pink hybrid（PDm52×PDm99）were randomly selected as the segregating population of the color of pink fruiting body and PDm17 as the tester line,the test-cross population was constructed. Fruiting results showed that the color of the test-cross population was obviously segregated in a continuous distribution pattern varying from dark to light with pink,light pink,white,etc. The results revealed that the pink of the fruiting body was not completely dominant with respect to the white,and the white was recessive. Color of fruiting body of P. djamor is a quantitative trait controlled by multiple genes with high heritability. Meanwhile,a significant correlation was detected between the colors of the cap and gill.

Key words: Pleurotus djamor, color of the fruiting body, quantitative trait, test-cross, hybridization

张泽华, 高巍, 邬向丽, 黄晨阳, 常明昌, 张金霞. 桃红侧耳子实体颜色的遗传规律研究[J]. 生物技术通报, 2019, 35(5): 70-75.

ZHANG Ze-hua, GAO Wei, WU Xiang-li, HUANG Chen-yang, CHANG Ming-chang, ZHANG Jin-xia. Inheritance Study on Fruiting Body Color of Pleurotus djamor（Rumph. ex Fr.）Boedijn[J]. Biotechnology Bulletin, 2019, 35(5): 70-75.

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桃红侧耳子实体颜色的遗传规律研究

Inheritance Study on Fruiting Body Color of Pleurotus djamor（Rumph. ex Fr.）Boedijn

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