不同日龄鸭肌肉组织多胺代谢和肌肉发育相关基因表达的规律

doi:10.13560/j.cnki.biotech.bull.1985.2021-1303

摘要/Abstract

摘要：

为研究并阐明鸭肌肉组织多胺代谢和肌肉发育相关基因表达的变化规律,利用高效液相色谱和实时荧光定量检测了0-120日龄鸭胸肌和腿肌组织中多胺含量、多胺代谢和肌肉发育相关基因表达量,并分析了多胺代谢和肌肉发育相关基因表达量的相关性。结果表明,0日龄鸭胸肌和腿肌组织中腐胺、亚精胺和精胺含量最高,胸肌和腿肌组织中多胺合成代谢相关基因分别呈一定规律变化;胸肌组织中,MyoD1和Myf5基因在0日龄时表达量最高,MyoG和IGF-1基因表达量在30日龄时最高,MSTN在90日龄时表达最高,且MyoD1和Myf5基因与SPMS、SPDS、SSAT、SMO基因呈显著的正相关;腿肌中,ODC与MyoD1和MSTN基因表达量呈显著的正相关。上述研究结果提示,鸭肌肉组织中多胺含量、多胺代谢和肌肉发育相关基因表达量的变化规律具有一定相似性,可为多胺调控鸭肌肉发育作用机制的研究提供参考。

关键词: 多胺, 代谢, 肌肉发育相关基因, 鸭

Abstract:

In order to study and clarify the expression changes of genes related to polyamine metabolism and muscle development in ducks’ muscle tissue,high performance liquid chromatography and RT-qPCR method were used to detect the polyamine contents and the expressions of polyamine metabolism and muscle development-related genes in the breast and leg muscle tissues during duck postnatal stage on day 0 to 120,and then to analyze the correlation between the expressions of muscle development-related gene and polyamine metabolism. Results showed that the contents of putrescine,spermidine and spermine in the duck breast and leg muscle at 0 days were the highest,and the polyamine metabolism genes in the breast and leg muscle tissue showed regular changes,respectively. In pectoral muscle tissue,the expressions of MyoD1 and Myf5 were the highest in 0 days,MyoG and IGF-1 were the highest at 30 days,and MSTN had the highest expression at 90 days,and MyoD1 and Myf5 genes were significantly and positively correlated with SPMS,SPDS,SSAT,and SMO genes. In leg muscle,ODC was significantly and positively correlated with MyoD1 and MSTN gene. The above results suggest that the changes in polyamine content,polyamine metabolism and muscle development-related gene expression in duck muscle tissues present a certain similarity,so as to provide the reference for studying polyamine regulating duck muscle development action mechanisms.

Key words: polyamine, metabolism, muscle development related genes, duck

周雪敏, 康丽鹃, 郭永妮, 杨笑含, 蒋易龙, 王泽龙, 孙倩, 康波. 不同日龄鸭肌肉组织多胺代谢和肌肉发育相关基因表达的规律[J]. 生物技术通报, 2022, 38(8): 244-251.

ZHOU Xue-min, KANG Li-juan, GUO Yong-ni, YANG Xiao-han, JIANG Yi-long, WANG Ze-long, SUN Qian, KANG bo. Expressions of Genes Related to Polyamine Metabolism and Muscle Development in Muscle Tissues of Duck at Different Ages[J]. Biotechnology Bulletin, 2022, 38(8): 244-251.

图/表 9

表1 实验所用引物序列

Table 1 Primer sequences used in the study

引物 Primer	序列 Sequence（5'-3'）	产物大小 Amplicon size/bp	退火温度 Annealing temperature/℃
MyoD1	F：GCAACGCCATCCGCTACAT R：GCAATCAAGGCTGGAAACAACA	85	64.5
Myf5	F：AGGAGGAGGCTGAAGAAAGTGA R：GCTCTGTCTCGGCAGGTGATA	180	60
MyoG	F：CGGATCACCTCCTGCCTGA R：CGTCCTCTACGGCGATGCT	87	63
IGF-1	F：GTGAAGATGCATACTGTGTC R：TGAAGTAAAAGCCTCTGT	249	60
MSTN	F：GCACTGGTATTTGGCAGAGTATT R：TCACCTGGTCCTGGGAAAGT	143	55
ODC	F：TTGACTGCCACATCCTTG R：GCTCTGCTATCGTTACACT	199	58
SPDS	F：ACCAGCTCATGAAGACAGCACTCA R：TGCTACACAGCATGAAGCCGATCT	189	60
SPMS	F：TTCGGGTGACTCAGTTCCTGCTAA R：AACGGAGACCCTCCTTCAGCAAAT	199	60
APAO	F：AGTCTTCACATGTGCTCTGTGGGT R：TGGCAATTGTGGGTTTCCTGTCAC	131	59
SSAT	F：TGCCGGTGTAGACAATGACAACCT R：TAAAGCTTTGGAATGGGTGCTCGC	114	59
SMO	F：CTACCCACGGTGCTGTGCTTT R：TTGAGCCCACCTGTGTGTAGGAAT	129	59
GAPDH	F：GTGGTGCAAGAGGCATTGCTGAC R：GCTGATGCTCCCATGTTCGTGAT	86	65

图1 不同日龄鸭胸肌组织多胺含量的变化规律图中不同小写字母表示在 P<0.05 水平上差异显著,下同

Fig. 1 Changes of polyamine contents of different aged duck pectoralis Different lowercase letters in the figure indicate significant differences at the P<0.05 level,the same below

图2 不同日龄鸭腿肌组织多胺含量的变化规律

Fig. 2 Changes of polyamine contents in different aged duck leg muscles

图3 不同日龄鸭胸肌中多胺代谢相关基因表达量变化

Fig. 3 Expression changes of of polyamine metabolism related gene in different aged duck pectoralis

图4 不同日龄鸭腿肌中多胺代谢相关基因表达量变化

Fig. 4 Expression changes of polyamine metabolism related gene in different aged duck leg muscles

图5 不同日龄鸭胸肌中MyoD1、Myf5、MyoG、IGF-1和MSTN基因表达量变化

Fig. 5 Changes of MyoD1,Myf5,MyoG,IGF-1 and MSTN gene expressions in different aged duck pectorales

图6 不同日龄鸭腿肌中MyoD1、Myf5、MyoG和MSTN基因表达量变化

Fig. 6 Expression changes of MyoD1,Myf5,MyoG and MSTN gene in different aged duck leg muscles

表2 胸肌中肌肉发育相关基因与多胺代谢基因表达量的相关系数

Table 2 Correlation coefficient between expressions of polyamine metabolism and muscle development genes in breast muscle

基因 Gene		相关系数 Correlation coefficient	P值 P value
MyoD1	SPMS	0.733 24	6.827 11×10^-4
MyoD1	SPDS	0.846 36	3.594 06×10^-7
MyoD1	SSAT	0.810 69	5.133 09×10^-7
MyoD1	SMO	0.820 04	1.720 74×10^-5
Myf5	SPMS	0.946 4	7.152 42×10^-10
Myf5	SPDS	0.827 96	3.259×10^-7
Myf5	SSAT	0.945 63	0.001 08
Myf5	SMO	0.916 11	3.682 54×10^-8

表3 腿肌中肌肉发育相关基因与多胺代谢基因表达量的相关系数

Table 3 Correlation coefficient between expressions of polyamine metabolism and muscle development genes in leg muscle

基因 Gene		相关系数 Correlation coefficient	P值 P value
MyoD1	ODC	0.746 32	0.005 53
MSTN	ODC	0.919 39	4.878 05×10^-12

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