甜菜BvBADH基因家族全基因组鉴定及其高盐胁迫下的表达分析

doi:10.13560/j.cnki.biotech.bull.1985.2023-0874

摘要/Abstract

摘要：

【目的】甜菜碱醛脱氢酶（betaine aldehyde dehydrogenase, BADH）是参与甘氨酸甜菜碱（glycine betaine, GB）合成的关键酶之一，在植物响应逆境胁迫过程中发挥重要作用。【方法】为探究甜菜（Beta vulgaris）BvBADH 基因家族成员生物学功能及基因表达模式，本研究采用生物信息学方法，分析了蛋白质的理化性质、系统进化、基因结构、染色体定位、保守基序、蛋白结构、启动子顺式作用调控元件，并通过RT-qPCR对其在盐胁迫下的表达模式进行分析。【结果】共鉴定出9个BvBADH 基因家族成员，含有9-15个外显子，8-14个内含子，平均氨基酸个数为516，平均分子量为55.84 kD，等电点为5.24-6.98。系统进化分析发现，高等植物BADH可分为3个簇，分别为簇I、II和III，其中簇III成员进一步分为簇IIIa和IIIb两个亚簇。BvBADH基因家族在3个簇中均有分布，分别含有3、1和5个成员。甜菜BvBADH基因家族主要含有胁迫响应元件、激素响应元件和生长发育响应元件，每个成员的顺式作用调控元件数量为13-21个。进一步对 BvBADH在盐处理下甜菜叶片中的表达模式分析发现，100和150 mmol/L NaCl不同程度地诱导BvBADH基因家族成员的表达；但相比之下，它们在100 mmol/L NaCl处理下的表达量高于150 mmol/L NaCl。【结论】BvBADH基因家族在甜菜响应盐胁迫过程中扮演着重要角色，为我国北方地区农作物抗逆性遗传改良提供优异基因资源。

关键词: 甜菜, 嗜盐作物, 甘氨酸甜菜碱, 甜菜碱醛脱氢酶, 顺式作用调控元件, 盐胁迫, 抗逆性

Abstract:

【Objective】 Betaine aldehyde dehydrogenase（BADH）is one of the key enzymes involved in glycine betaine synthesis and plays an important role in plant response to abiotic stress.【Method】 To explore the biological function and gene expression pattern of BvBADH gene family members in sugar beet（Beta vulgaris）, the physicochemical properties of protein, phylogenetic evolution, gene structure, chromosome localization, conserved motif, protein structure, and promoter cis-acting regulatory elements of BvBADHs were analyzed by bioinformatics method, and their expression patterns under salt stress were investigated by RT-qPCR.【Result】 A total of 9 BvBADH gene family members were identified. They contained 9-15 exons and 8-14 introns, the average amino acid number was 516, the average molecular weight was 55.84 kD, and the isoelectric point was 5.24-6.98. Phylogenetic analysis found that BADHs of higher plants were divided into three clusters, namely Clusters I, II, and III, among which Cluster III members can be further divided into two subgroups, including Cluster IIIa and IIIb. The members of BvBADH gene family were distributed in 3 clusters, respectively containing 3, 1, and 5 members. The promoter of the BvBADH gene mainly contained stress response elements, hormone response elements and growth and development response elements, the cis-acting regulatory element numbers of each member were 13-21. Further analysis of the expression pattern of the BvBADH genes in the sugar beet leaves showed that both 100 and 150 mmol/L NaCl significantly increased the expressions of all members of BvBADHs, but their expressions under 100 mmol/L NaCl were higher than those under 150 mmol/L NaCl.【Conclusion】 The BvBADH gene family plays an important role in sugar beet's response to salt stress, it provides excellent genetic resources for the genetic improvement of crop stress tolerance in northern China.

Key words: sugar beet（Beta vulgaris）, halophilic crops, glycine betaine, betaine aldehyde dehydrogenase, cis-acting regulatory element, salt stress, stress resistance

李昊, 伍国强, 魏明, 韩悦欣. 甜菜BvBADH基因家族全基因组鉴定及其高盐胁迫下的表达分析[J]. 生物技术通报, 2024, 40(2): 233-244.

LI Hao, WU Guo-qiang, WEI Ming, HAN Yue-xin. Genome-wide Identification of the BvBADH Gene Family in Sugar Beet（Beta vulgaris）and Their Expression Analysis Under High Salt Stress[J]. Biotechnology Bulletin, 2024, 40(2): 233-244.

图/表 12

参考文献 61

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基因Gene	正向引物序列Forward primer sequence（5'-3'）	反向引物序列Reverse primer sequence（5'-3'）
BvBADH1	GCAGTGCAACATCCAGACTC	CCCCCTCACTCTTTGCTGTT
BvBADH2	GTCCTGTTGTCAGCAAGGGA	GCATGGACGTGGAGACATCA
BvBADH3	CCAAATTGGGGGAAGTTTGTGT	TGGATCACCAACAACCCAAGA
BvBADH4	ACGGGGAATTTGTGGATGCT	TCATCACCCTTCCCCTTTCA
BvBADH5	ATTGAGCATGGCAAGCGAGA	TGCCATTCGCTCGCTCTATC
BvBADH6	GCCGATTTAGCACCACTTCT	GCAGAAGTGGTGCTAAATCGG
BvBADH7	GCATCTGATTATGGGCTGGC	CCACTCATTTTGTACCCGCC
BvBADH8	TCGTCGAGCCAATAACACCC	CCACCAAATGGAATGCCTGC
BvBADH9	GGCCAGTTCAGAGGTGAGGA	GAACTTGGACCCAAGCCTTTT
BvACTIN	ACTGGTATTGTGCTTGACTC	ATGAGATAATCAGTGAGATC

基因Gene	正向引物序列Forward primer sequence（5'-3'）	反向引物序列Reverse primer sequence（5'-3'）
BvBADH1	GCAGTGCAACATCCAGACTC	CCCCCTCACTCTTTGCTGTT
BvBADH2	GTCCTGTTGTCAGCAAGGGA	GCATGGACGTGGAGACATCA
BvBADH3	CCAAATTGGGGGAAGTTTGTGT	TGGATCACCAACAACCCAAGA
BvBADH4	ACGGGGAATTTGTGGATGCT	TCATCACCCTTCCCCTTTCA
BvBADH5	ATTGAGCATGGCAAGCGAGA	TGCCATTCGCTCGCTCTATC
BvBADH6	GCCGATTTAGCACCACTTCT	GCAGAAGTGGTGCTAAATCGG
BvBADH7	GCATCTGATTATGGGCTGGC	CCACTCATTTTGTACCCGCC
BvBADH8	TCGTCGAGCCAATAACACCC	CCACCAAATGGAATGCCTGC
BvBADH9	GGCCAGTTCAGAGGTGAGGA	GAACTTGGACCCAAGCCTTTT
BvACTIN	ACTGGTATTGTGCTTGACTC	ATGAGATAATCAGTGAGATC

基因名称 Gene name	基因登录号 Gene ID	CDS /bp	相对分子量 Mw/kD	亲水性平均值 GRAVY	等电点 pI	脂肪指数 Aliphatic index	氨基酸数目 Protein length/aa	不稳定指数 Instability index
BvBADH1	Bv5_ 116250_aodi	1 500	54.72	-0.077	5.45	88.78	500	32.48
BvBADH2	Bv5_ 116230_ntjn	1 512	54.78	-0.062	5.37	88.51	503	31.62
BvBADH3	Bv8_ 195870_rfyd	1 509	54.69	-0.081	5.24	87.45	502	28.42
BvBADH4	Bv8_ 195860_yiyp	1 506	54.25	-0.079	5.60	89.00	501	22.07
BvBADH5	Bv8_ 195850_giqy	1 509	54.34	-0.039	6.01	88.05	502	25.97
BvBADH6	Bv5_ 116750_zqhy	1 605	58.11	-0.111	6.65	85.90	534	31.08
BvBADH7	Bv6_ 149040_dgrz	1 605	57.84	-0.076	6.53	89.55	534	32.99
BvBADH8	Bv6_ 149060_fmfu	1 608	58.71	-0.109	6.54	85.74	535	32.04
BvBADH9	Bv2_047750_ktxe	1 602	57.11	0.066	6.98	95.72	533	38.12

基因名称 Gene name	基因登录号 Gene ID	CDS /bp	相对分子量 Mw/kD	亲水性平均值 GRAVY	等电点 pI	脂肪指数 Aliphatic index	氨基酸数目 Protein length/aa	不稳定指数 Instability index
BvBADH1	Bv5_ 116250_aodi	1 500	54.72	-0.077	5.45	88.78	500	32.48
BvBADH2	Bv5_ 116230_ntjn	1 512	54.78	-0.062	5.37	88.51	503	31.62
BvBADH3	Bv8_ 195870_rfyd	1 509	54.69	-0.081	5.24	87.45	502	28.42
BvBADH4	Bv8_ 195860_yiyp	1 506	54.25	-0.079	5.60	89.00	501	22.07
BvBADH5	Bv8_ 195850_giqy	1 509	54.34	-0.039	6.01	88.05	502	25.97
BvBADH6	Bv5_ 116750_zqhy	1 605	58.11	-0.111	6.65	85.90	534	31.08
BvBADH7	Bv6_ 149040_dgrz	1 605	57.84	-0.076	6.53	89.55	534	32.99
BvBADH8	Bv6_ 149060_fmfu	1 608	58.71	-0.109	6.54	85.74	535	32.04
BvBADH9	Bv2_047750_ktxe	1 602	57.11	0.066	6.98	95.72	533	38.12

顺式作用元件Cis-acting element	功能Function	序列Sequence	BvBADH
顺式作用元件Cis-acting element	功能Function	序列Sequence	1	2	3	4	5	6	7	8	9
ABRE	Abscisic acid-responsive element	ACGTG	3	2	5	3	3	2	0	4	3
TCA-element	Involved in salicylic acid responsiveness	CCATCTTTTT	0	1	0	1	0	0	2	0	1
TGA-element	Auxin-responsive element	AACGAC	0	0	1	1	0	1	0	1	0
ARE	Anaerobic-responsive element	AAACCA	4	3	1	2	4	4	5	2	6
LTR	Low-temperature responsiveness	CCGAAA	1	0	0	2	0	0	2	0	2
Box 4	Involved in light responsiveness	ATTAAT	3	3	2	1	0	1	0	3	1
GT1-motif	Involved in light responsiveness	GGTTAAT	2	0	4	3	4	0	2	2	1
TCT-motif	Involved in light responsiveness	TCTTAC	6	0	2	1	1	1	1	2	0
AE-box	Modul for light response	AGAAACTT	0	1	0	2	0	1	0	0	0
O2-site	Zein metabolism regulation	GATGACATGA	1	1	0	0	0	1	0	1	0
G-box	Involved in light responsiveness	TACGTG	1	2	7	0	4	4	3	0	0