丁香罗勒（Ocimum gratissimum）叶片响应镉胁迫的比较转录组学分析

doi:10.13560/j.cnki.biotech.bull.1985.2024-0618

摘要/Abstract

摘要：

目的丁香罗勒（Ocimum gratissimum）同时具有修复和安全利用镉（cadmium, Cd）污染土壤的潜力，解析丁香罗勒响应Cd胁迫的分子机制，鉴定与Cd耐受性密切相关的调控因子，为耐受Cd胁迫的丁香罗勒种质资源创新提供候选基因资源。方法采用比较转录组学的方法，分析Cd处理（35 μmol/L）72 h期间丁香罗勒叶片的转录组学变化。结果 Cd胁迫显著抑制丁香罗勒生长，叶片、茎和根中均积累了很高含量的Cd，根中的Cd含量最高。Cd胁迫还显著影响精油组分和含量，Cd处理诱导肉桂酸甲酯的合成积累。转录组分析结果显示，在24 h的比较组中，差异表达基因（differentially expressed genes, DEGs）富集在核糖体、植物-病原相互作用和MAPK信号通路等途径中。在72 h的比较组中，DEGs富集在光合作用、光合作用-天线蛋白和卟啉与叶绿素代谢途径中。浅青色1模块内的基因与Cd胁迫表型显著相关，模块内的基因主要富集在植物激素信号转导、植物-病原相互作用和MAPK信号通路途径中。Cd胁迫上调表达的DEGs也显著富集在这3个途径，表明激活这3个途径是丁香罗勒叶片应答Cd胁迫的主要机制。Cd胁迫处理诱导13个转录因子基因表达上调，其中5个是bHLH家族基因，表明bHLH转录因子在丁香罗勒Cd胁迫耐受性调控中具有重要作用。结论丁香罗勒应答Cd胁迫的分子机制与其他植物有很大相似之处，bHLH转录因子可能是调控丁香罗勒Cd耐受性的关键转录因子。

关键词: 丁香罗勒, 镉胁迫, RNA-Seq, 光合作用, bHLH转录因子

Abstract:

Objective Clove basil (Ocimum gratissimum) has the potential for both remediation and safe utilization of cadmium (Cd)-contaminated soil. Elucidating the molecular mechanisms of clove basil in response to Cd stress and identifying key regulatory factors closely associated with Cd tolerance may provide candidate gene resources for the innovation of germplasm resources of clove basil tolerant to Cd stress. Method This study employed comparative transcriptome to analyze the transcriptomic changes in clove basil leaves during 72 h of Cd treatment (35 μmol/L). Result Cd stress significantly inhibited the growth of clove basil. The leaves, stems, and roots of clove basil accumulated high levels of Cd, with the highest content in the roots. Additionally, Cd stress significantly affected the composition and content of essential oils, inducing the biosynthesis and accumulation of methyl ester cinnamic acid in the leaves. The transcriptome analysis results showed that differentially expressed genes (DEGs) were significantly enriched in pathways such as ribosome, plant-pathogen interaction, and the MAPK signaling pathway in the 24 h comparative group. Whereas DEGs were significantly enriched in pathways including photosynthesis, photosynthesis-antenna proteins, and porphyrin and chlorophyll metabolism in the 72 h comparative group. Genes within the light cyan1 module were significantly correlated with the Cd stress phenotype. These genes were primarily enriched in defense pathways such as plant hormone signal transduction, plant-pathogen interaction, and the MAPK signaling pathway. Furthermore, the upregulated DEGs in response to Cd stress were also significantly enriched in these three pathways, suggesting that the activation of these pathways was the main mechanism by which clove basil leaves responded to Cd stress. Cd stress treatment induced the expressions of 13 transcription factor genes, in which 5 genes from the bHLH family, suggesting crucial roles for bHLH transcription factors in regulating Cd stress tolerance in clove basil. Conclusion The molecular mechanisms by which clove basil responds to Cd stress closely resemble those of other plant species, and the bHLH transcription factor appears to act as key regulators of Cd tolerance in clove basil.

Key words: clove basil, cadmium stress, RNA-Seq, photosynthesis, bHLH transcription factor

王斌, 王玉昆, 肖艳辉. 丁香罗勒（Ocimum gratissimum）叶片响应镉胁迫的比较转录组学分析[J]. 生物技术通报, 2025, 41(3): 255-270.

WANG Bin, WANG Yu-kun, XIAO Yan-hui. Comparative Transcriptomic Analysis of Clove Basil （Ocimum gratissimum） Leaves in Response to Cadmium Stress[J]. Biotechnology Bulletin, 2025, 41(3): 255-270.

图/表 13

参考文献 37

1	綦峥, 齐越, 杨红, 等. 土壤重金属镉污染现状、危害及治理措施 [J]. 食品安全质量检测学报, 2020, 11(7): 2286-2294.
	Qi Z, Qi Y, Yang H, et al. Status, harm and treatment measures of heavy metal cadmium pollution in soil [J]. J Food Saf Qual, 2020, 11(7): 2286-2294.
2	Satarug S, Baker JR, Urbenjapol S, et al. A global perspective on cadmium pollution and toxicity in non-occupationally exposed population [J]. Toxicol Lett, 2003, 137(1/2): 65-83.
3	陈能场, 郑煜基, 何晓峰, 等. 《全国土壤污染状况调查公报》探析 [J]. 农业环境科学学报, 2017, 36(9): 1689-1692.
	Chen NC, Zheng YJ, He XF, et al. Analysis of the report on the national general survey of soil contamination [J]. J Agro Environ Sci, 2017, 36(9): 1689-1692.
4	Gavrilescu M. Enhancing phytoremediation of soils polluted with heavy metals [J]. Curr Opin Biotechnol, 2022, 74: 21-31.
5	Wang B, Lin LN, Yuan X, et al. Low-level cadmium exposure induced hormesis in peppermint young plant by constantly activating antioxidant activity based on physiological and transcriptomic analyses [J]. Front Plant Sci, 2023, 14: 1088285.
6	Wang B, Wang YK, Yuan X, et al. Comparative transcriptomic analysis provides key genetic resources in clove basil (Ocimum gratissimum) under cadmium stress [J]. Front Genet, 2023, 14: 1224140.
7	郭向阳. 6种食用芳香植物挥发性成分的GC-MS/GC-O分析 [J]. 农业工程学报, 2019, 35(18): 299-307.
	Guo XY. Analysis of volatile compositions in six edible fragrant plants by GC-MS/GC-O technology [J]. Trans Chin Soc Agric Eng, 2019, 35(18): 299-307.
8	Hu ZY, Zhang YF, He Y, et al. Full-length transcriptome assembly of Italian ryegrass root integrated with RNA-seq to identify genes in response to plant cadmium stress [J]. Int J Mol Sci, 2020, 21(3): 1067.
9	Feng SJ, Shen YH, Xu HN, et al. RNA-seq identification of Cd responsive transporters provides insights into the association of oxidation resistance and Cd accumulation in Cucumis sativus L [J]. Antioxidants (Basel), 2021, 10(12): 1973.
10	He F, Liu QQ, Zheng L, et al. RNA-seq analysis of rice roots reveals the involvement of post-transcriptional regulation in response to cadmium stress [J]. Front Plant Sci, 2015, 6: 1136.
11	Li YK, Ding LH, Zhou M, et al. Transcriptional regulatory network of plant cadmium stress response [J]. Int J Mol Sci, 2023, 24(5): 4378.
12	张晓娜, 朴春兰, 董友魁, 等. 大豆根系应答重金属Cd胁迫的转录组分析 [J]. 应用生态学报, 2017, 28(5): 1633-1641.
	Zhang XN, Piao CL, Dong YK, et al. Transcriptome analysis of response to heavy metal Cd stress in soybean root [J]. Chin J Appl Ecol, 2017, 28(5): 1633-1641.
13	Liu ZC, Zhou LZ, Gan CC, et al. Transcriptomic analysis reveals key genes and pathways corresponding to Cd and Pb in the hyperaccumulator Arabis paniculata [J]. Ecotoxicol Environ Saf, 2023, 254: 114757.
14	肖艳辉, 李应文, 邹碧, 等. 钝化剂抑制南方污染农田籽粒苋吸收重金属的效应研究 [J]. 生态环境学报, 2021, 30(4): 825-833.
	Xiao YH, Li YW, Zou B, et al. Reduction of heavy metal uptake by amaranth by 3 soil amendments in contaminated farmland of South China [J]. Ecol Environ Sci, 2021, 30(4): 825-833.
15	王斌, 袁晓, 蒋园园, 等. bHLH96的克隆及其在薄荷萜烯生物合成调控中的功能 [J]. 生物技术通报, 2024, 40(1): 281-293.
	Wang B, Yuan X, Jiang YY, et al. Cloning of bHLH96 gene and its roles in regulating the biosynthesis of peppermint terpenes [J]. Biotechnol Bull, 2024, 40(1): 281-293.
16	Geng YY, Qin LK, Liu YN, et al. Ultrastructure observation and transcriptome analysis of Chinese chestnut (Castanea mollissima Blume) seeds in response to water loss [J]. Food Biosci, 2021, 42: 101095.
17	Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C(T) method [J]. Nat Protoc, 2008, 3(6): 1101-1108.
18	Langfelder P, Horvath S. WGCNA: an R package for weighted correlation network analysis [J]. BMC Bioinformatics, 2008, 9: 559.
19	宋琳, 刘梦宇, 周航, 等. 镉砷胁迫下玉米幼苗的生理响应和抗氧化系统特征 [J]. 农业环境科学学报, 2024, 43(4): 752-762.
	Song L, Liu MY, Zhou H, et al. Physiological response and antioxidant system characteristics of maize (Zea mays L.) seedlings under cadmium and arsenic stress [J]. J Agro Environ Sci, 2024, 43(4): 752-762.
20	Xu J, Yin HX, Liu XJ, et al. Salt affects plant Cd-stress responses by modulating growth and Cd accumulation [J]. Planta, 2010, 231(2): 449-459.
21	张慧, 张欣雨, 袁旭, 等. 烟草叶片响应镉胁迫的差异表达基因鉴定及分析 [J]. 作物学报, 2024, 50(4): 944-956.
	Zhang H, Zhang XY, Yuan X, et al. Transcriptome analysis of tobacco in response to cadmium stress [J]. Acta Agron Sin, 2024, 50(4): 944-956.
22	Yan JY, Wu XZ, Li T, et al. Effect and mechanism of nano-materials on plant resistance to cadmium toxicity: a review [J]. Ecotoxicol Environ Saf, 2023, 266: 115576.
23	Khanna K, Kohli SK, Ohri P, et al. Agroecotoxicological aspect of Cd in soil-plant system: uptake, translocation and amelioration strategies [J]. Environ Sci Pollut Res Int, 2022, 29(21): 30908-30934.
24	Yu XF, Liu YJ, Yang L, et al. Low concentrations of methyl jasmonate promote plant growth and mitigate Cd toxicity in Cosmos bipinnatus [J]. BMC Plant Biol, 2024, 24(1): 807.
25	李源恒, 赵春莉, 郭宏亮, 等. 镉胁迫对黄秋英生理及富集特性的影响 [J]. 山东农业科学, 2024, 56(1): 81-90.
	Li YH, Zhao CL, Guo HL, et al. Effects of cadmium stress on physiological and enrichment characteristics of Cosmos sulphureus [J]. Shandong Agric Sci, 2024, 56(1): 81-90.
26	Tong WY, Ahmad Rafiee AR, Leong CR, et al. Development of sodium alginate-pectin biodegradable active food packaging film containing cinnamic acid [J]. Chemosphere, 2023, 336: 139212.
27	Barroso JP, de Almeida AA F, do Nascimento JL, et al. The damage caused by Cd toxicity to photosynthesis, cellular ultrastructure, antioxidant metabolism, and gene expression in young cacao plants are mitigated by high Mn doses in soil [J]. Environ Sci Pollut Res Int, 2023, 30(54): 115646-115665.
28	Kou M, Xiong J, Li M, et al. Interactive effects of Cd and Pb on the photosynthesis efficiency and antioxidant defense system of Capsicum annuum L [J]. Bull Environ Contam Toxicol, 2022, 108(5): 917-925.
29	Wang SQ, Dai HP, Cui S, et al. The effects of salinity and pH variation on hyperaccumulator Bidens pilosa L. accumulating cadmium with dynamic and real-time uptake of Cd²⁺ influx around its root apexes [J]. Environ Sci Pollut Res Int, 2023, 30(14): 41435-41444.
30	刘琛, 林义成, 郭彬, 等. 丛枝菌根真菌通过改变植物基因表达水平和微生物群落结构促进红叶石楠对镉的吸收 [J]. 生物工程学报, 2022, 38(1): 287-302.
	Liu C, Lin YC, Guo B, et al. Arbuscular mycorrhizal fungi enhanced cadmium uptake in Photinia frase through altering root transcriptomes and root-associated microbial communities [J]. Chin J Biotechnol, 2022, 38(1): 287-302.
31	Khan A, Jie Z, Kong XJ, et al. Pre treatment of melatonin rescues cotton seedlings from cadmium toxicity by regulating key physio-biochemical and molecular pathways [J]. J Hazard Mater, 2023, 445: 130530.
32	Li X, Xu B, Sahito ZA, et al. Transcriptome analysis reveals cadmium exposure enhanced the isoquinoline alkaloid biosynthesis and disease resistance in Coptis chinensis [J]. Ecotoxicol Environ Saf, 2024, 271: 115940.
33	Song L, Huang SSC, Wise A, et al. A transcription factor hierarchy defines an environmental stress response network [J]. Science, 2016, 354(6312): aag1550.
34	Zhang HW, Lu LL. Transcription factors involved in plant responses to cadmium-induced oxidative stress [J]. Front Plant Sci, 2024, 15: 1397289.
35	Khan I, Asaf S, Jan R, et al. Genome-wide annotation and expression analysis of WRKY and bHLH transcriptional factor families reveal their involvement under cadmium stress in tomato (Solanum lycopersicum L.) [J]. Front Plant Sci, 2023, 14: 1100895.
36	Yao YN, He ZQ, Li XM, et al. Genome-wide identification of bHLH gene family and its response to cadmium stress in Populus × canescens [J]. PeerJ, 2024, 12: e17410.
37	Du XY, Fang LH, Li JX, et al. The TabHLH094-TaMYC8 complex mediates the cadmium response in wheat [J]. Mol Breed, 2023, 43(7): 57.

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基因编号 Gene ID	正向引物序列 Forward primer sequence （5΄‒3΄）	反向引物序列 Reverse primer sequence （5΄‒3΄）	产物长度 Product length/bp
c52549.graph_c0	ATCGAGCAGAGATGAAGCCG	TTGACGCGCGTGTTTTTCAT	134
c49765.graph_c0	AACAAGAGGGCGGAAGGAAG	AGAGAAAGAGGGGCTGTTGC	73
c57224.graph_c0	AAGGCGCTGGAATCGGATAG	GCGATTTGCACTCCACGTTT	125
c59334.graph_c0	CACAATTCCGATCGCACGAC	TGGCCTCCAAGTTATGCTGG	71
c51522.graph_c0	CGGTTCGTCTCCTTGGTGAA	AGTCTCCTCGACCAGCTCAT	119
c54882.graph_c1	AGGATGGTGTGAGGAGAGCA	TGCTCAAGTGGAGGATGCAA	140
Actin7	GGAGCTCGTCTTTGCTGTCT	GAGCGGGAAATTGTGAGGGA	90

基因编号 Gene ID	正向引物序列 Forward primer sequence （5΄‒3΄）	反向引物序列 Reverse primer sequence （5΄‒3΄）	产物长度 Product length/bp
c52549.graph_c0	ATCGAGCAGAGATGAAGCCG	TTGACGCGCGTGTTTTTCAT	134
c49765.graph_c0	AACAAGAGGGCGGAAGGAAG	AGAGAAAGAGGGGCTGTTGC	73
c57224.graph_c0	AAGGCGCTGGAATCGGATAG	GCGATTTGCACTCCACGTTT	125
c59334.graph_c0	CACAATTCCGATCGCACGAC	TGGCCTCCAAGTTATGCTGG	71
c51522.graph_c0	CGGTTCGTCTCCTTGGTGAA	AGTCTCCTCGACCAGCTCAT	119
c54882.graph_c1	AGGATGGTGTGAGGAGAGCA	TGCTCAAGTGGAGGATGCAA	140
Actin7	GGAGCTCGTCTTTGCTGTCT	GAGCGGGAAATTGTGAGGGA	90

序号 No.	化合物名称 Compound name	保留时间 Retention time/min	含量 Content/%		序号 No.	化合物名称 Compound name	保留时间 Retention time/min	含量 Content/%
序号 No.	化合物名称 Compound name	保留时间 Retention time/min	CK	Cd	序号 No.	化合物名称 Compound name	保留时间 Retention time/min	CK	Cd
1	3-己烯醛 3-hexenal	4.13	1.86±0.16*	0.68±0.04	26	顺式-肉桂酸甲酯 Methyl cis-cinnamate	12.00	-	5.13±0.26
2	（E）-2-己烯醛（E）-2-hexenal	4.96	0.25±0.01*	0.08±0.00	27	丁香酚Eugenol	12.71	25.7±1.41*	0.02±0.01
3	顺式-3-己烯醇 Cis-3-hexenol	5.00	1.54±0.41*	0.14±0.04	28	肉桂酸甲酯 Methyl ester cinnamic acid	13.06	0.06±0.10	58.02±2.02*
4	α-侧柏烯 α-thujene	6.16	0.15±0.02*	0.06±0.01	29	β-榄香烯 β-elemene	13.21	0.83±0.09*	0.31±0.04
5	α-蒎烯 α-pinene	6.28	0.38±0.02*	0.21±0.01	30	甲基丁香酚 Methyl eugenol	13.28	6.56±0.14*	2.04±0.31
6	莰烯 Camphene	6.54	0.06±0.01	0.02±0.00	31	β-佛手柑油烯 β-bergamotene	13.49	0.10±0.01	0.05±0.01
7	香桧烯 Sabinene	6.93	0.14±0.01*	0.08±0.01	32	石竹烯 Caryophyllene	13.64	0.31±0.02*	0.16±0.01
8	β-蒎烯β-pinene	7.00	0.38±0.04*	0.19±0.01	33	反式-α-佛手柑油烯 Trans-α-bergamotene	13.76	2.41±0.11	2.09±0.05
9	β-月桂烯 β-myrcene	7.18	0.02±0.01	0.10±0.00*	34	顺式-β-金合欢烯Cis-β-farnesene	13.83	0.21±0.02	0.08±0.02
10	D-柠檬烯 D-limonene	7.83	0.32±0.00*	0.15±0.00	35	反式-β-金合欢烯 Trans-β-farnesene	13.95	0.29±0.03*	0.19±0.02
11	桉树脑 Eucalyptol	7.89	3.02±0.11*	1.95±0.09	36	蛇麻烯 Humulene	14.08	1.27±0.04*	0.70±0.02
12	（E）-β-罗勒烯（E）-β-ocimene	8.10	2.27±0.11*	1.13±0.02	37	顺式-4（15），5-依兰油二烯 Cis-muurola-4（15），5-diene	14.18	0.25±0.05*	0.08±0.01
13	γ-松油烯 γ-terpinene	8.30	0.40±0.02*	0.14±0.01	38	大根香叶烯 D Germacrene D	14.41	2.52±0.04*	1.18±0.02
14	顺式-水合香桧烯Cis-sabinene hydrate	8.46	0.79±0.05*	0.27±0.01	39	β-环大根香叶烷 β-cyclogermacrane	14.61	1.54±0.09*	0.4±0.01
15	萜品油烯 Terpinolene	8.78	0.14±0.01*	0.01±0.02	40	δ-愈创木烯 δ-guaiene	14.69	0.21±0.02*	0.06±0.02
16	葑酮 Fenchone	8.82	0.23±0.02	0.23±0.05	41	γ-杜松烯 γ-cadinene	14.80	1.07±0.04*	0.56±0.02
17	芳樟醇 Linalool	8.92	12.10±0.51*	4.15±0.19	42	β-倍半水芹烯 β-sesquiphellandrene	14.86	0.11±0.03	0.06±0.01
18	（+）-2-莰酮（+）-2-bornanone	9.71	0.85±0.03*	0.25±0.01	43	α-杜松烯 α-cadinene	15.01	0.09±0.02	0.03±0.00
19	新薄荷醇 Neo-menthol	9.97	0.12±0.01*	0.03±0.00	44	反式-橙花叔醇 Trans-nerolidol	15.28	0.41±0.21*	0.06±0.00
20	δ-松油醇 δ-terpineol	10.02	0.09±0.01	0.04±0.00	45	表荜澄茄油烯醇 Epicubenol	16.03	0.20±0.02*	0.08±0.01
21	4-萜品醇 L-terpinen-4-ol	10.17	4.46±0.76*	2.65±0.12	46	τ-杜松醇 τ-cadinol	16.31	2.36±0.04*	0.98±0.02
22	L-α-松油醇 L-α-terpineol	10.36	1.35±0.10*	0.68±0.02	47	吲哚-3-乙醛 Indole-3-acetaldehyde	16.96	0.08±0.03	-
23	4-烯丙基苯甲醚 Estragole	10.46	20.50±1.47*	13.9±2.26	48	新植二烯 Neophytadiene	18.29	0.69±0.10*	0.31±0.03
24	乙酸辛酯 Octyl ester acetic acid	10.57	0.32±0.04*	0.16±0.03	49	n-十六酸 n-hexadecanoic acid	19.46	0.18±0.01*	0.08±0.00
25	3-乙烯基-4-甲基-1H- 吡咯-2，5-二酮 3-ethenyl-4-methyl-1H-pyrrole-2，5-dione	11.25	0.17±0.01*	0.05±0.00	合计 Total amount/%			99.67	99.91

序号 No.	化合物名称 Compound name	保留时间 Retention time/min	含量 Content/%		序号 No.	化合物名称 Compound name	保留时间 Retention time/min	含量 Content/%
序号 No.	化合物名称 Compound name	保留时间 Retention time/min	CK	Cd	序号 No.	化合物名称 Compound name	保留时间 Retention time/min	CK	Cd
1	3-己烯醛 3-hexenal	4.13	1.86±0.16*	0.68±0.04	26	顺式-肉桂酸甲酯 Methyl cis-cinnamate	12.00	-	5.13±0.26
2	（E）-2-己烯醛（E）-2-hexenal	4.96	0.25±0.01*	0.08±0.00	27	丁香酚Eugenol	12.71	25.7±1.41*	0.02±0.01
3	顺式-3-己烯醇 Cis-3-hexenol	5.00	1.54±0.41*	0.14±0.04	28	肉桂酸甲酯 Methyl ester cinnamic acid	13.06	0.06±0.10	58.02±2.02*
4	α-侧柏烯 α-thujene	6.16	0.15±0.02*	0.06±0.01	29	β-榄香烯 β-elemene	13.21	0.83±0.09*	0.31±0.04
5	α-蒎烯 α-pinene	6.28	0.38±0.02*	0.21±0.01	30	甲基丁香酚 Methyl eugenol	13.28	6.56±0.14*	2.04±0.31
6	莰烯 Camphene	6.54	0.06±0.01	0.02±0.00	31	β-佛手柑油烯 β-bergamotene	13.49	0.10±0.01	0.05±0.01
7	香桧烯 Sabinene	6.93	0.14±0.01*	0.08±0.01	32	石竹烯 Caryophyllene	13.64	0.31±0.02*	0.16±0.01
8	β-蒎烯β-pinene	7.00	0.38±0.04*	0.19±0.01	33	反式-α-佛手柑油烯 Trans-α-bergamotene	13.76	2.41±0.11	2.09±0.05
9	β-月桂烯 β-myrcene	7.18	0.02±0.01	0.10±0.00*	34	顺式-β-金合欢烯Cis-β-farnesene	13.83	0.21±0.02	0.08±0.02
10	D-柠檬烯 D-limonene	7.83	0.32±0.00*	0.15±0.00	35	反式-β-金合欢烯 Trans-β-farnesene	13.95	0.29±0.03*	0.19±0.02
11	桉树脑 Eucalyptol	7.89	3.02±0.11*	1.95±0.09	36	蛇麻烯 Humulene	14.08	1.27±0.04*	0.70±0.02
12	（E）-β-罗勒烯（E）-β-ocimene	8.10	2.27±0.11*	1.13±0.02	37	顺式-4（15），5-依兰油二烯 Cis-muurola-4（15），5-diene	14.18	0.25±0.05*	0.08±0.01
13	γ-松油烯 γ-terpinene	8.30	0.40±0.02*	0.14±0.01	38	大根香叶烯 D Germacrene D	14.41	2.52±0.04*	1.18±0.02
14	顺式-水合香桧烯Cis-sabinene hydrate	8.46	0.79±0.05*	0.27±0.01	39	β-环大根香叶烷 β-cyclogermacrane	14.61	1.54±0.09*	0.4±0.01
15	萜品油烯 Terpinolene	8.78	0.14±0.01*	0.01±0.02	40	δ-愈创木烯 δ-guaiene	14.69	0.21±0.02*	0.06±0.02
16	葑酮 Fenchone	8.82	0.23±0.02	0.23±0.05	41	γ-杜松烯 γ-cadinene	14.80	1.07±0.04*	0.56±0.02
17	芳樟醇 Linalool	8.92	12.10±0.51*	4.15±0.19	42	β-倍半水芹烯 β-sesquiphellandrene	14.86	0.11±0.03	0.06±0.01
18	（+）-2-莰酮（+）-2-bornanone	9.71	0.85±0.03*	0.25±0.01	43	α-杜松烯 α-cadinene	15.01	0.09±0.02	0.03±0.00
19	新薄荷醇 Neo-menthol	9.97	0.12±0.01*	0.03±0.00	44	反式-橙花叔醇 Trans-nerolidol	15.28	0.41±0.21*	0.06±0.00
20	δ-松油醇 δ-terpineol	10.02	0.09±0.01	0.04±0.00	45	表荜澄茄油烯醇 Epicubenol	16.03	0.20±0.02*	0.08±0.01
21	4-萜品醇 L-terpinen-4-ol	10.17	4.46±0.76*	2.65±0.12	46	τ-杜松醇 τ-cadinol	16.31	2.36±0.04*	0.98±0.02
22	L-α-松油醇 L-α-terpineol	10.36	1.35±0.10*	0.68±0.02	47	吲哚-3-乙醛 Indole-3-acetaldehyde	16.96	0.08±0.03	-
23	4-烯丙基苯甲醚 Estragole	10.46	20.50±1.47*	13.9±2.26	48	新植二烯 Neophytadiene	18.29	0.69±0.10*	0.31±0.03
24	乙酸辛酯 Octyl ester acetic acid	10.57	0.32±0.04*	0.16±0.03	49	n-十六酸 n-hexadecanoic acid	19.46	0.18±0.01*	0.08±0.00
25	3-乙烯基-4-甲基-1H- 吡咯-2，5-二酮 3-ethenyl-4-methyl-1H-pyrrole-2，5-dione	11.25	0.17±0.01*	0.05±0.00	合计 Total amount/%			99.67	99.91

样品编号 Sample No.	Clean reads	GC含量 GC content/%	≥Q30/%	比对读数 Mapped reads	比对比率 Mapped ratio/%
0 h CK-1	22 830 057	49.84	93.02	17 200 093	75.34
0 h CK-2	22 391 222	49.80	92.93	17 070 648	76.24
0 h CK-3	21 181 042	50.05	93.88	16 240 183	76.67
24 h CK-1	21 485 915	50.28	93.29	16 070 383	74.79
24 hCK-2	21 898 153	50.27	93.37	16 540 511	75.53
24 h CK-3	21 872 539	50.81	93.37	16 650 456	76.12
72 h CK-1	22 127 839	49.96	93.08	16 819 392	76.01
72 h CK-2	20 937 711	50.20	93.83	15 897 564	75.93
72 h CK-3	19 962 929	50.29	93.46	15 148 810	75.88
24 h Cd-1	25 353 059	49.97	93.80	19 449 566	76.71
24 h Cd-2	20 283 780	49.74	93.89	15 626 179	77.04
24 h Cd-3	20 638 087	49.93	93.68	15 794 277	76.53
72 h Cd-1	20 774 226	49.12	93.32	15 927 385	76.67
72 h Cd-2	26 674 376	48.82	92.73	19 912 643	74.65
72 h Cd-3	22 262 126	48.62	93.14	16 647 781	74.78