一株耐盐好氧反硝化细菌的分离筛选及鉴定

生物技术通报 ›› 2013, Vol. 0 ›› Issue (3): 175-180.

一株耐盐好氧反硝化细菌的分离筛选及鉴定

石小彤,李彦芹,邢国伟,康现江

河北大学生命科学学院，保定 071002

收稿日期:2012-11-23 修回日期:2013-03-21 出版日期:2013-03-20 发布日期:2013-03-21
作者简介:石小彤，女，硕士研究生，研究方向：水生生物学；E-mail ：shi.880902@yahoo.com.cn
基金资助:
海洋公益性行业科研专项经费子课题（201305001-4）

Screening and Primary Identification of an Aerobic Denitrifier Isolate with Salt Tolerance

Shi Xiaotong, Li Yanqin, Xing Gongwei, Kang Xianjiang

College of Life Sciences,Hebei University,Baoding 071002）

Received:2012-11-23 Revised:2013-03-21 Published:2013-03-20 Online:2013-03-21

摘要/Abstract

摘要： 对对虾养殖池底泥进行富集分离，从4 株反硝化细菌中筛选出一株高效的好氧反硝化菌株F1。该菌能在好氧条件下， 40 h 内高效地将硝酸盐浓度由442.28 mg/L 降至58.28 mg/L，亚硝酸盐浓度由32.79 mg/L 降至0.98 mg/L，反硝化作用主要发生在对数生长期。经菌落形态学特征、生理生化反应及 16S rDNA 分子鉴定，初步鉴定为盐单胞菌。

关键词: 好氧反硝化细菌, 反硝化效率, 盐单胞菌, 耐盐

Abstract: Selective enrichment culture method under oxygen conditions was used for screening bacteria,the 4 strains which have capability of aerobic denitrification was isolated from a shrimp pond. A strong denitrifying effect bacteria was selected from them（F1）. The bacterium reduced the concentrations of nitrate nitrogen rapidly from 442.28 mg/L to 58.28 mg/L, nitrite nitrogen from 32.79 mg/L to 0.98 mg/L with 40 h in aerobic conditions. It identified as Halomonas sp. based on its biochemical and morphological characters. Phylogenetic analysis of 16S rDNA sequences indicated that strain F1 was most closely related to Halomonas alimentaria. The denitrification mainly occurs in the logarithmic growth phase.

Key words: Aerobic denitrifier, Denitrification efficiency, Halomonas sp., Salt tolerance

石小彤,李彦芹,邢国伟,康现江. 一株耐盐好氧反硝化细菌的分离筛选及鉴定[J]. 生物技术通报, 2013, 0(3): 175-180.

Shi Xiaotong, Li Yanqin, Xing Gongwei, Kang Xianjiang. Screening and Primary Identification of an Aerobic Denitrifier Isolate with Salt Tolerance[J]. Biotechnology Bulletin, 2013, 0(3): 175-180.

参考文献

[1] 文屹. 两株异养硝化- 好氧反硝化细菌的分离、筛选、鉴定和特性研究[D]. 广州:华南理工大学, 2010.
[2] Robertson LA, Kuenen JG. Aerobic denitrification :a controversy revived [J]. Arch Microbiol, 1984, 139 :351-354.
[3] Chen F, Xia Q, Ju LK. Aerobic denitrification of Pseudomonas aeruginosa monitored by online nad(p)h fluorescence [J]. Applied and Environmental Microbiology, 2003, 69(11):6715- 6722.
[4] 马放, 周丹丹, 王宏宇, 等. 一株好氧反硝化细菌生理生态特征的研究[J]. 哈尔滨工业大学学报, 2006, 38(4):575-577.
[5] Pai SL, Chong NM, Chen CH. Potential applications of aerobic denitrifying bacteria as bioagents in wastewater treatment [J]. Bioresource Technology, 1999, 68(2):179-185.
[6] Kim JK, Park KJ, Cho KS, et al. Aerobic nitrifcation-denitrifcation 100 58 100 85 100 34 40 38 71 57 Halomonas hydrothermalis??AF212218?? Halomonas venusta??AJ306894?? Halomonas boliviensis??AY245449?? Halomonas gomseomensis??AM229314?? Halomonas subterranea??EF144148?? F1 Halomonas alimentaria??AF211860T?? Halomonas campisalis??AF054286?? Halomonas desiderata??X92417?? Halomonas eurihalina??X87218?? Chromohalobacter canadensis??AJ295143?? Halomonas halocynthiae??AJ417388?? Carnomonas nigrifaciens??Y13299?? 0.01 图6 菌株F1 的系统发育树生物技术通报 Biotechnology Bulletin 2013年第3期180 by heterotrophic Bacillus strains [J]. Bioresource Technology, 2005, 96(17):1897-1906.
[7] 杨基先, 高珊珊, 马放, 等. 一株好氧反硝化细菌的分离鉴定及反硝化能力[J]. 环境科学学报, 2008, 28(7):1302-1307.
[8] Takaya N, Maria Antonina BCS, Yasushi S, et al. Aerobic denitrifying bacteria that produce low levels of nitrous oxide [J]. Applied and Environmental Microbiology, 2003, 69(6):3152-3157.
[9] 国家环保局编委会. 水和废水监测分析方法 [M]. 第3 版. 北京: 中国环境科学出版社, 1989.
[10] 东秀珠, 蔡妙英, 等. 常见细菌系统鉴定手册[M]. 北京:科学出版社, 2001.
[11] 王薇, 蔡祖聪, 钟文辉, 等. 好氧反硝化菌的研究进展[J]. 应用生态学报, 2007, 18(11):2618-2625.
[12] Zumft WG. Cell biology and molecular basis of denitrification [J]. Microbiol Mol Biol Rev, 1997, 61(4):533-616.
[13] 廖绍安, 郑桂丽, 王安利, 等. 养虾池好氧反硝化细菌新菌株的分离鉴定及特征[J]. 生态学报, 2006, 26(11):3178- 3724.
[14] Li J, Wang W, Liang L, et al. Screening of aerobic denitrifying bacteria with salt tolerance and characteristics of aerobic denitrification [J]. Environmental Science &Technology, 2011, 34(6):48-52.
[15] 周丹丹, 马放, 王弘宇, 等. 关于好氧反硝化菌筛选方法的研究[J]. 微生物学报, 2004, 44(6):837-839.