沙根骨中碳酸钙矿化微生物分离筛选及鉴定

生物技术通报 ›› 2014, Vol. 0 ›› Issue (2): 171-175.

沙根骨中碳酸钙矿化微生物分离筛选及鉴定

胡伟莲, 戴德慧

浙江科技学院生物与化学工程学院，杭州 310023

收稿日期:2013-09-03 出版日期:2014-02-27 发布日期:2014-02-27
作者简介:胡伟莲，女，博士，副教授，研究方向：微生物生物技术；E-mail：weilian89@126.com
基金资助:
浙江省自然科学基金资助项目（LY12C01004 ）

The Isolation，Screening and Identification of CaCO₃ Mineralization Strain from Sand Boon

Hu Weilian, Dai Dehui

（Department of Biology and Chemical Engineering，Zhejiang Universtity of Science and Technology，Hangzhou 310023）

Received:2013-09-03 Published:2014-02-27 Online:2014-02-27

摘要/Abstract

摘要： 沙根骨（自命名）是在宁夏中卫地区发现的以碳酸钙为主的胶结物质将周围的沙子凝聚在一起形管状物。对沙根骨中碳酸钙矿化微生物进行了分离筛选，共分离到12株菌株，其中3#菌株有较强碳酸钙矿化能力，在经1.0% CaCl₂液体培养基中振荡培养的72 h后，能形成矿物沉淀并形成薄膜挂壁。沉淀物质经扫描电镜（SEM）及X射线粉末衍射（XRD）分析，确定为方解石和球霞石混合晶型的碳酸钙晶体。根据其形态特征、生理生化以及16S rDNA对3#菌株进行了鉴定，初步认定了该菌为类芽孢杆菌属，命名为Paenibacillus sp.s3，为进一步研究沙根骨的形成机理奠定了良好基础。

关键词: 沙根骨, 生物矿化, 微生物, 类芽孢杆菌属

Abstract: Sand bone（named by the research team）is a tube that is agglutinated by sand with cementing material mainly composed of calcium carbonate. It was found in Zhongwei area of Ningxia Autonomous Region. Strain 3# has strong ability of CaCO₃ mineralization among 12 strains isolated from sand bone. Mineral deposit was produced and covered the flask wall after 72 h shake cultivation with 1.0% CaCl₂ liquid medium. To analyze the mineral deposits, XRD and SEM were performed. The result was showed that mineral deposit was made up of calcium carbonate, which contains two crystal forms（calcite and vaterite）. Based on morphological, physiological and biochemical properties and 16S rDNA sequence analysis, strain 3# was identified as Paenibacillus sp., and was named Paenibacillus sp. s3 . It laid a good foundation for reaching formation mechanism of sand boon further.

胡伟莲, 戴德慧. 沙根骨中碳酸钙矿化微生物分离筛选及鉴定[J]. 生物技术通报, 2014, 0(2): 171-175.

Hu Weilian, Dai Dehui. The Isolation，Screening and Identification of CaCO₃ Mineralization Strain from Sand Boon[J]. Biotechnology Bulletin, 2014, 0(2): 171-175.

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