盐胁迫下沙冬青细胞端粒酶活性的变化与DNA稳定性的关系

生物技术通报 ›› 2014, Vol. 0 ›› Issue (10): 134-138.

盐胁迫下沙冬青细胞端粒酶活性的变化与DNA稳定性的关系

张徐俞¹, 王瑾瑜², 郑广顺³, 张俊琦¹, 卢存福¹

1.北京林业大学生物科学与技术学院分析测试中心林木育种国家工程实验室,北京 100083; 2.清华大学分析测试中心,北京 100084; 3.中国科学院植物研究所,北京 100093

收稿日期:2014-03-12 出版日期:2014-10-20 发布日期:2014-10-17
作者简介:张徐俞,女,硕士研究生,研究方向：植物端粒、端粒酶
基金资助:
高等学校学科创新引智计划资助项目（B13007）,长江学者和创新团队发展计划资助项目（IRT13047）,北京市教委共建项目（101009）、北京市公园管理中心课题（0710016）,北京市自然科学基金项目（6112016）

Effects of Salt Stress on Telomerase Activity in Relation to DNA Stability of Ammopiptanthus mongolicus Cells

Zhang Xuyu¹, Wang Jinyu², Zheng Guangshun³, Zhang Junqi¹, Lu Cunfu¹

1. College of Biological Sciences and Biotechnology,Analysis and Testing Center,National Engineering Laboratory for Tree Breeding,Beijing Forestry University,Beijing 100083; 2. Analysis and Testing Center,Tsinghua University,Beijing 100084; 3. Institute of Botany,Chinese Academy of Sciences,Beijing 100093

Received:2014-03-12 Published:2014-10-20 Online:2014-10-17

摘要/Abstract

摘要： 沙冬青（Ammopiptanthus mongolicus）是亚洲中部荒漠地区的常绿阔叶灌木,是古老的第三纪孑遗植物,具有极强的抗逆能力。旨在探究盐胁迫下沙冬青细胞端粒酶活性变化与染色体DNA稳定性的关系。结果表明,在100 mmol/L NaCl的低浓度盐胁迫处理下,随着处理时间的增加,端粒酶活性没有增加,未出现明显的DNA降解现象;当在500 mmol/L高浓度盐胁迫时,处理的初期端粒酶活性迅速的增加,但随着处理时间的延长,端粒酶活性下降,此时DNA未明显降解;当移除NaCl胁迫,端粒酶活性增加1.4倍,DNA保持稳定。因此推测,在盐胁迫下,沙冬青细胞端粒酶活性的维持对避免细胞遭受不可逆损伤,保持染色体DNA稳定性具有一定的作用。

关键词: 沙冬青, 盐胁迫, 端粒酶, DNA损伤

Abstract: Ammopiptanthus mongolicus, the evergreen broadleaf shrub indigenous to the northwest desert of China, is a residual plant of the ancient subtropical area in the Tertiary Period and has been identified as the national tertiary protection species. This research was conducted to explore the relationship between DNA damage and telomerase activity of Ammopiptanthus mongolicus cells under salt stress. The results showed that telomerase activity was increased during the first 3 d of low salt（100 mmol/L NaCl）treatment. However, when culture cells were treated with 500 mmol/L NaCl, telomerase activity increased rapidly at the initial stage, while declined after 2 day salt stress. Telomerase activity increased 1.4-fold in the recovery phase when 500 mmol/L NaCl was removed from the growth medium. DNA damage was not obvious during the NaCl treatment time or in the phase when NaCl was removed from the growth medium. It is proposed that plants might have developed a highly efficient DNA repair system to cope with transient salt stress, and telomerase may play an important role in it.

Key words: Ammopiptanthus mongolicus, Salt stress, Telomerase, DNA damage

张徐俞, 王瑾瑜, 郑广顺, 张俊琦, 卢存福. 盐胁迫下沙冬青细胞端粒酶活性的变化与DNA稳定性的关系[J]. 生物技术通报, 2014, 0(10): 134-138.

Zhang Xuyu, Wang Jinyu, Zheng Guangshun, Zhang Junqi, Lu Cunfu. Effects of Salt Stress on Telomerase Activity in Relation to DNA Stability of Ammopiptanthus mongolicus Cells[J]. Biotechnology Bulletin, 2014, 0(10): 134-138.

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