TAP亲和标签选择及其在植物蛋白互作研究中的应用

摘要/Abstract

摘要：

串联亲和纯化法（TAP）是一种纯化生理条件下蛋白复合物的技术，已广泛应用于鉴定蛋白相互作用和揭示蛋白复合物互作网络。近年来，随着TAP新型标签的不断出现以及与其他技术的联用，TAP技术正逐渐应用于植物蛋白质互作研究中。综述了TAP亲和标签选择，并介绍其在植物蛋白互作研究中的成功应用。

关键词: 串联亲和纯化, 亲和标签, 植物, 蛋白质相互作用

Abstract:

Tandem affinity purification（TAP）is a technology for the purification of protein complex under the normal physiological conditions, which has been extensively utilized to identify protein-protein interactions（PPIs）and reveal interaction network of protein complexes. In recent years, TAP technology has been improving in the PPIs study, with the development of new labels, TAP method improvements and jointed use with other technologies associated. TAP technology is also being increasingly used in the studies of plant PPIs. This paper reviews the selection of TAP affinity tag and advance of research that TAP has been successful used in the study of plant PPIs.

Key words: Tandem affinity purification, Tag, Plant, Protein-protein interactions

张志飞, 赵志丽, 文昭竹. TAP亲和标签选择及其在植物蛋白互作研究中的应用[J]. 生物技术通报, 2014, 0(8): 23-27.

Zhang Zhifei, Zhao Zhili, Wen Zhaozhu. Tag Selection of Tandem Affinity Purification and Its Application in Plant Protein-Protein Interactions[J]. Biotechnology Bulletin, 2014, 0(8): 23-27.

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