CRISPR/Cas基因编辑技术及其在真菌中的应用

doi:10.13560/j.cnki.biotech.bull.1985.2017.03.009

摘要/Abstract

摘要： CRISPR/Cas系统是细菌和古生菌中抵抗外源病毒或质粒入侵的获得性免疫系统。其中II型CRISPR/Cas系统已被改造成为一个简便、高效的基因编辑工具,并在动物、植物和微生物基因功能研究和遗传改良中得到广泛应用。简述了CRISPR/Cas系统的结构、作用机理及分类情况,归纳总结了CRISPR/Cas9系统在酿酒酵母和其他丝状真菌中的应用,并对该技术可能出现的问题及应用前景进行了展望,以期为真菌基因编辑研究提供参考。

关键词: 真菌, 基因组编辑技术, CRISPR/Cas9, 基因功能

Abstract: CRISPR/Cas system is a self-defense system against exogenous virus or plasmid in bacteria and archaea. CRISPR/Cas9,an expeditious genome editing technology,was developed according to the type II CRISPR/Cas system. Nowadays,the CRISPR/Cas9 genome editing technology has broadly utilized in gene function and genetic modification research in animal,plant and microorganism. Here,we briefly introduce the structure,mechanism and classification of CRISPR/Cas system,describe the application of CRISPR/Cas9 genome editing technology in fungi,and analyze the advantages,disadvantages as well as the prospect and application values of this technology. It is aimed at providing a useful reference for researchers in genetic modification of fungi.

Key words: fungi, genome editing technology, CRISPR/Cas9, gene function

殷朝敏, 范秀芝, 史徳芳, 高虹. CRISPR/Cas基因编辑技术及其在真菌中的应用[J]. 生物技术通报, 2017, 33(3): 58-65.

YIN Chao-min, FAN Xiu-zhi, SHI De-fang, GAO Hong. CRISPR/Cas Genome Editing Technology and Its Application in Fungi[J]. Biotechnology Bulletin, 2017, 33(3): 58-65.

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