植物类甜蛋白基因家族研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2017-0774

摘要/Abstract

摘要： 类甜蛋白（Thaumatin-like proteins，TLPs）在植物对抗胁迫过程中发挥重要作用。该家族属多基因家族，蛋白分子量较小，具有典型的thaumatin结构域，且高度保守；典型的TLP蛋白由16个半胱氨酸残基对形成8个二硫键，三维解析结构具有功能域I、II和III 3个保守功能域，表面具有“V”形酸性裂缝，保证了蛋白的催化功能；多数物种TLP蛋白归为10个聚类组，各组发生了不均衡扩增；TLP蛋白具有抗真菌、葡聚糖酶、过敏原等活性，能被激素、逆境胁迫等多种信号诱导表达，能被生物或非生物因子诱导表达，广泛参与了植物生长发育、抵御胁迫等多项生命进程，在植物先天免疫中发挥作用。通过基因工程手段，越来越多具有抗真菌潜力的TLP家族基因被用于提高植物抗病性。从类甜蛋白结构、进化、生物学功能及其应用等方面对近期研究成果进行了综述，以期为后续研究提供参考。

关键词: 类甜蛋白, 抗真菌活性, 转基因, 植物胁迫

Abstract: Thaumatin-like proteins（TLPs）play an important role in the process of plant resistance to stress. TLP family belongs to multi-gene one，and its molecular weight is small，and it has typical thaumatin domain of highly conserved. Typical TLP is composed of 16 cysteine residues forming eight disulfide bonds，and the three-dimensional structure possesses three conserved domains I，II，and III，forming a “V” shape acidic cleft on the surface，which ensure the catalytic function of TLP. Most species’ TLPs are classified into 10 clusters，and unbalanced amplification occurs in each cluster. TLPs possess antifungal，glucanase，and allergens activities，and can be induced by biotic or abiotic factors，such as hormones，stress signal，thus they play a role in the life process and plant innate immunity. Growingly TLP family genes with antifungal potential have been used to improve plant disease resistance through genetic engineering. In this study，we summarized recent researches on the structure，evolution，biological function and application of TLPs，so as to provide a theoretical basis for further study.

Key words: thaumatin-like protein, antifungal activity, transgene, plant stress

刘潮, 韩利红, 王海波, 高永, 唐利洲. 植物类甜蛋白基因家族研究进展[J]. 生物技术通报, 2018, 34(3): 9-17.

LIU Cha, HAN Li-hong, WANG Hai-bo, GAO Yong, TANG Li-zhou. Research Advances on Plant Thaumatin-like Protein Family[J]. Biotechnology Bulletin, 2018, 34(3): 9-17.

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