一株海洋微小链霉菌群体感应抑制活性及培养条件的研究

doi:10.13560/j.cnki.biotech.bull.1985.2019-0341

生物技术通报 ›› 2019, Vol. 35 ›› Issue (10): 137-143.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0341

一株海洋微小链霉菌群体感应抑制活性及培养条件的研究

周恒¹, 姜芸¹, 许叶祥¹, 钱生辉¹, 缪莉^{1, 2}

1. 扬州大学环境科学与工程学院,扬州 225127;
2. 中国科学院热带海洋生物资源与生态重点实验室,广州 510301

收稿日期:2019-04-22 出版日期:2019-10-26 发布日期:2019-09-30
作者简介:周恒,男,硕士研究生,研究方向：海洋微生物生物活性及天然活性物质;E-mail：970069241@qq.com
基金资助:
广东省海洋药物重点实验室开放课题基金及中国科学院热带海洋生物资源与生态重点实验室开放课题基金（2018011008）

Research on Quorum Sensing Inhibitory Activity and Culture Condition of a Marine Streptomyces parvulus

ZHOU Heng¹, JIANG Yun¹, XU Ye-xiang¹, QIAN Sheng-hui¹, MIAO Li^{1, 2}

1. College of Environmental Science and Engineering,Yangzhou University,Yangzhou 225127;
2. Key Laboratory of Tropical Marine Biological Resources and Ecology,Chinese Academy of Sciences,Guangzhou 510301

Received:2019-04-22 Published:2019-10-26 Online:2019-09-30

摘要/Abstract

摘要： 以一株分离自连云港海区表层海水中的海洋微小链霉菌（Streptomyces parvulus HY026）为研究对象,采用滤纸片法对其进行群体感应抑制活性测试。结果显示S. parvulus HY026的代谢产物能显著抑制群体感应报告菌紫色杆菌产紫色素,表现出较强的群体感应抑制活性;采用单因子实验,对HY026进行培养基碳源及氮源的优化。发现以玉米粉为碳源时,所得粗提物的质量及群体感应抑制活性最高;以黄豆粉为氮源时,所得粗提物的质量及其活性最高。根据单因子实验结果,选取玉米粉和黄豆粉为最佳碳、氮源,连同盐度、磷酸氢二钾共4个因素进行L16（4⁵）正交实验,以各处理所得的发酵液粗提物质量和群体感应活性为指标进行分析。确定4个因素的重要性顺序为磷酸氢二钾>盐度>玉米粉>黄豆粉,最佳培养基配方为：磷酸氢二钾1.0 g/L,盐度34.0 g/L,玉米粉10.0 g/L,黄豆粉5.0 g/L。以此配方培养时,与原高氏一号培养基培养相比,S. parvulus HY026的发酵液粗提物质量和群体感应抑制活性分别提高了398%和19%。

关键词: 海洋微生物, 微小链霉菌, 群体感应, 条件优化

Abstract: We used filter paper method to investigate the quorum sensing inhibitory activity and culture condition of a marine Actinomycetes Streptomyces parvulus（HY026）that was isolated from Lianyungang surface water in our previous study. It was found that,the metabolites of S. parvulus（HY026）significantly inhibited the violacein production in the quorum sensing reporter bacterium Chromobacterium violaceum 12472,showing the strong inhibition to quorum sensing activity. The carbon and nitrogen sources of HY026 medium were optimized by single factor experiment. It was revealed that the quality and quorum sensing inhibitory activity of the HY026 crude extract were the highest when corn meal was used as carbon source,and the quality and activity of the crude extract were the highest when soybean meal was used as nitrogen source. According to the results of the single factor experiment,corn meal and soybean meal were selected as the best carbon and nitrogen sources,and 4 factors,including salinity and potassium hydrogen phosphate,were used to carry out L16（4⁵）orthogonal experiment. The quality and quorum sensing activity of the crude extracts from each treatment were analyzed. The order of importance of the four factors was as potassium hydrogen phosphate > salinity > corn flour > soybean flour. The optimal medium recipe was potassium hydrogen phosphate 1.0 g/L,salinity 34.0 g/L,corn flour 20.0 g/L and soybean flour 5.0 g/L. The quality and quorum sensing inhibitory activity of the crude extracts from S. parvulus HY026 fermentation broth increased by 398% and 19%,respectively,when cultured in this optimal recipe.

Key words: marine microorganisms, Streptomyces parvulus, quorum sensing, condition optimization

周恒, 姜芸, 许叶祥, 钱生辉, 缪莉. 一株海洋微小链霉菌群体感应抑制活性及培养条件的研究[J]. 生物技术通报, 2019, 35(10): 137-143.

ZHOU Heng, JIANG Yun, XU Ye-xiang, QIAN Sheng-hui, MIAO Li. Research on Quorum Sensing Inhibitory Activity and Culture Condition of a Marine Streptomyces parvulus[J]. Biotechnology Bulletin, 2019, 35(10): 137-143.

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一株海洋微小链霉菌群体感应抑制活性及培养条件的研究

Research on Quorum Sensing Inhibitory Activity and Culture Condition of a Marine Streptomyces parvulus

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