关键词: 银杏, 愈伤组织, 正交实验, 生长, 褐化, 黄酮

Abstract: The aim of this study is to establish a stable callus succession system of Ginkgo biloba,to screen the condition of culturing cells with high activity and flavonoids,and thus to provide references for industrial production. Using MS medium as the basic medium,we investigated the G. biloba callus growth,browning and flavonoid accumulation under the interactions among explant month,exogenous hormone and anti-browning agent by an orthogonal experiment of 40 d succession period,and compared with the contents of G. biloba leaves’ flavonoids in April,May,and June. The results showed that the condition for optimal growth of G. biloba callus was callus induced by April leaf + 3.0 mg/L NAA + 0.5 mg/L KT + 5 mg/L VC. The optimal anti-browning condition was callus induced by April leaf +1.0 mg/L NAA+1.0 mg/L KT+10 mg/L VC. The flavonoid content of G. biloba leaf tended to increase from April to June,and the flavonoid content of callus at different month was positively correlated with its explants,the condition of callus induced by June leaf + 2.0 mg/L NAA + 1.0 mg/L KT + 5 mg/L VC was the optimal for flavonoids accumulation. Combined with the dry weight of callus,the maximum flavonoid yield was from the combination of callus induced by May leaf + 2.0 mg/L NAA + 0.5 mg/L KT + 5 mg/L VC. The appropriate succession period for G. biloba callus was 24-32 d,with large growth and low browning rate.

Key words: Ginkgo biloba L., callus, orthogonal experiment, growth, browning, flavonoids