动态调控：一种高效的细胞工厂工程化代谢改造策略

doi:10.13560/j.cnki.biotech.bull.1985.2020-0317

摘要/Abstract

摘要： 动态调控作为代谢工程优化中最有效的策略之一,通常包含输入信号发生器、生物传感器和执行机构3个部分。输入信号可以是细胞代谢物和环境条件变化,如化学分子、核糖核酸、温度、光信号等。而生物传感器是能够响应输入信号变化,并转化成特定信号输出的基因元件,其输出信号可以直接调控基因表达,也可以作为其他感应元件的输入。重点介绍了动态调控的基本原理及分类及其在微生物细胞工厂工程化改造中的应用实例,主要包括动态调控的优势和研究进展、动态调控系统的构建与表征,同时,也着重讨论了动态调控在细胞工厂改造中潜在的机遇与可能面临的挑战。

关键词: 动态调控, 静态调控, 代谢工程改造, 生物传感器, 合成生物学, 微生物细胞工厂

Abstract: Dynamic control,consisting of 3 parts of input signal generator,biosensor and actuator,is one of the most efficient strategies for metabolic engineering optimization. The input signals can be metabolites and environmental changes,such as chemicals,RNA,temperature,light,etc. Biosensors are input signal-responsive genetic parts that transfer signals into specific ones that actuate the expression of target genes or can be used as input of downstream cascaded sensor systems. Here,we briefly introduce the basic principles and categories of dynamic control systems,and review the cases of microbial cell factory engineering by employing dynamic control,mainly including the advantages,recent progresses,construction and characterization. Meanwhile,we also discuss the potential opportunities and challenges of employing dynamic control in metabolically engineering microbial cell factories.

Key words: dynamic control, static control, metabolic engineering, biosensor, synthetic biology, microbial cell factory

叶健文, 陈江楠, 张旭, 吴赴清, 陈国强. 动态调控：一种高效的细胞工厂工程化代谢改造策略[J]. 生物技术通报, 2020, 36(6): 1-12.

YE Jian-wen, CHEN Jiang-nan, ZHANG Xu, Wu Fu-qing, CHEN Guo-qiang. Dynamic Control：An Efficient Strategy for Metabolically Engineering Microbial Cell Factories[J]. Biotechnology Bulletin, 2020, 36(6): 1-12.

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