生物技术通报 ›› 2021, Vol. 37 ›› Issue (8): 1-11.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0861

• 代谢生物学专题 •    下一篇

代谢组学在植物逆境生物学中的研究进展

张凤(), 陈伟()   

  1. 1.华中农业大学 作物遗传改良国家重点实验室 国家植物基因研究中心
    2.华中农业大学植物科学技术学院,武汉 430070
  • 收稿日期:2021-07-08 出版日期:2021-08-26 发布日期:2021-09-10
  • 作者简介:张凤,女,博士,研究方向:作物代谢组学;E-mail: zhangfeng@mail.hzau.edu.cn
  • 基金资助:
    华中农业大学科技技术自主创新基金资助项目(2017RC006)

Research Progress of Metabolomics in Plant Stress Biology

ZHANG Feng(), CHEN Wei()   

  1. 1. National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research,Huazhong Agricultural University, Wuhan 430070
    2. College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070
  • Received:2021-07-08 Published:2021-08-26 Online:2021-09-10

摘要:

近年随着持续而又复杂环境的改变,自然界中生物和非生物胁迫频繁爆发,多种逆境胁迫严重影响了植物的正常生长和发育,尤其是农作物产量。逆境胁迫下植物体内代谢物的重塑是其基因与环境因素共同作用的结果,是植物体生理表型与体内生化水平的直接体现,逆境胁迫下代谢组的重塑很大程度上反映了植物体对逆境胁迫的响应和防御。代谢组学的兴起,为研究植物体内不同组织及其在不同逆境胁迫下代谢物的重塑提供了可靠的研究手段,同时代谢组与基因组、转录组、蛋白组以及表型组的整合,尤其是代谢组与基因组整合形成的代谢组-基因组关联分析在揭示植物响应及适应逆境胁迫的遗传基础、提高农作物产量以及培育耐受逆境胁迫品种等方面具有重要作用。本文综述了逆境胁迫下植物代谢组学的研究方法、逆境胁迫下植物代谢组重塑的多样性以及逆境胁迫下植物代谢组的遗传基础研究进展,并展望了应用代谢组学研究植物逆境生物学的应用前景和局限性。

关键词: 代谢组学, 逆境胁迫, 代谢组重塑, 遗传基础, 调控机制

Abstract:

In recent years,with continuous and complex environmental changes,biotic and abiotic stresses frequently burst out in nature,and many stresses seriously affect plant normal growth and development,especially crop yield. The metabolic remodeling under stresses are the consequences of interactions between genotypes and surrounding environments,are the direct reflections of plant physiological phenotypes and biochemical activities,and largely reflects the plant response and defense to stresses. The rise of metabolomics provides a reliable way to study the metabolic remodeling in different tissues and under stresses in plants. Meanwhile,the integrations of metabolome with genome,transcriptome,proteome and phenome,especially metabolome-genome association analysis by the integration of genome and metabolome play an important role in revealing the genetic basis of plant response and adaptation to stresses,in improving crop yield and in developing stress-tolerant crop varieties. In this paper,the metabolome research method,the diversity of the metabolic remodeling and the genetic basis of plant metabolome under stresses are reviewed,and the application prospects and limitations of plant metabolomics in plant stress biology are also prospected.

Key words: metabolomics, stresses, metabolic remodeling, genetic basis, regulatory mechanism