生物技术通报 ›› 2022, Vol. 38 ›› Issue (2): 113-122.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0243
马青云1,2(), 江旭1, 李情情1,2, 宋金龙3, 周义清1, 阮志勇1,4,5()
收稿日期:
2021-03-03
出版日期:
2022-02-26
发布日期:
2022-03-09
作者简介:
马青云,女,硕士,研究方向:环境微生物;E-mail: 基金资助:
MA Qing-yun1,2(), JIANG Xu1, LI Qing-qing1,2, SONG Jin-long3, ZHOU Yi-qing1, RUAN Zhi-yong1,4,5()
Received:
2021-03-03
Published:
2022-02-26
Online:
2022-03-09
摘要:
为获得更丰富的烟嘧磺隆降解菌资源,以合肥某烟嘧磺隆生产厂活性污泥为研究对象,从中分离并筛选得到一株能以葡萄糖为唯一碳源、烟嘧磺隆为唯一氮源生长的菌株,根据其表型特征、16S rRNA序列相似性、DNA-DNA杂交值(DDH)和平均核苷酸一致性(ANI)的分析结果,将其鉴定为Chryseobacterium lacus LAM-M5。该菌株可在7 d内将初始浓度为50 mg/L的烟嘧磺隆降解92.39%。利用液相色谱-质谱联用(LC-MS)对菌株LAM-M5与烟嘧磺隆混合培养后的代谢产物进行检测与鉴定,共有5种主要物质被检测到,根据烟嘧磺隆的化学结构式和中间代谢产物特征,初步推测出菌株Chryseobacterium sp. LAM-M5降解烟嘧磺隆的可能降解途径。利用高效液相色谱(HPLC)对接菌后降解体系内代谢产生的酸类物质进行分离与鉴定,结果表明产物主要为L-苹果酸。推测菌株LAM-M5受高浓度烟嘧磺隆胁迫,利用葡萄糖产生L-苹果酸,通过降低环境 pH使烟嘧磺隆发生水解,从而解除其胁迫。通过对菌株全基因组信息进行分析,预测了7种可能与烟嘧磺隆降解相关的基因。结果表明,菌株LAM-M5 具有较强的烟嘧磺隆降解能力,在烟嘧磺隆污染环境的生物修复上具有较好的应用潜力。
马青云, 江旭, 李情情, 宋金龙, 周义清, 阮志勇. 烟嘧磺隆降解菌Chryseobacterium sp. LAM-M5的分离、鉴定及其降解机理研究[J]. 生物技术通报, 2022, 38(2): 113-122.
MA Qing-yun, JIANG Xu, LI Qing-qing, SONG Jin-long, ZHOU Yi-qing, RUAN Zhi-yong. Isolation and Identification of Nicosulfuron Degrading Strain Chryseobacterium sp. LAM-M5 and Study on Its Degradation Pathway[J]. Biotechnology Bulletin, 2022, 38(2): 113-122.
图4 不同pH值(A)、温度(B)、接种量(C)对菌株LAM-M5 降解烟嘧磺隆降解率的影响 图中误差线代表标准偏差,下同
Fig.4 Effects of different pH(A),temperature(B)and initial inoculum ratio(C)on the degradation of nicosulfuron by strain LAM-M5 The error line in the figurebars refers to standard errors. The same below
基因组组分 Characteristics | 菌株LAM-M5 Chryseobacterium lacus LAM-M5 |
---|---|
Genome size/bp | 6 311 696 |
Gene number | 6 366 |
Gene length/bp | 5 704 104 |
GC content/% | 43.84 |
genes of genome/% | 90.37 |
Gene average length/bp | 896 |
Gene internal length/bp | 607 592 |
Gene internal GC content/% | 36.52 |
Internal of genome/% | 9.63 |
表1 LAM-M5的基因组组分结果统计
Table 1 Genomic characteristics of strain LAM-M5
基因组组分 Characteristics | 菌株LAM-M5 Chryseobacterium lacus LAM-M5 |
---|---|
Genome size/bp | 6 311 696 |
Gene number | 6 366 |
Gene length/bp | 5 704 104 |
GC content/% | 43.84 |
genes of genome/% | 90.37 |
Gene average length/bp | 896 |
Gene internal length/bp | 607 592 |
Gene internal GC content/% | 36.52 |
Internal of genome/% | 9.63 |
编号 No. | 预测的降解酶基因 Predicted degeration genes | 大小 Size/bp |
---|---|---|
1 | Putative phosphoesterase | 172 |
2 | Acyl-CoA thioesterase YneP | 264 |
3 | Carboxylesterase | 254 |
4 | Catalase X | 535 |
5 | Probable manganese catalase | 274 |
6 | Hydrolase YhcX | 513 |
7 | Putative sporulation hydrolase CotR | 317 |
表2 菌株LAM-M5 基因组中7个降解酶相关的基因
Table 2 7 genes related to degradation enzymes in the genome of strain LAM-M5
编号 No. | 预测的降解酶基因 Predicted degeration genes | 大小 Size/bp |
---|---|---|
1 | Putative phosphoesterase | 172 |
2 | Acyl-CoA thioesterase YneP | 264 |
3 | Carboxylesterase | 254 |
4 | Catalase X | 535 |
5 | Probable manganese catalase | 274 |
6 | Hydrolase YhcX | 513 |
7 | Putative sporulation hydrolase CotR | 317 |
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