生物技术通报 ›› 2022, Vol. 38 ›› Issue (7): 119-127.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1184

• 综述与专论 • 上一篇    下一篇

天然类胡萝卜素的生物合成研究进展

周琳1,2(), 梁轩铭1, 赵磊1,2()   

  1. 1.中国科学院天津工业生物技术研究所,天津 300308
    2.中国科学院系统微生物工程重点实验室,天津 300308
  • 收稿日期:2021-09-14 出版日期:2022-07-26 发布日期:2022-08-09
  • 作者简介:周琳,女,硕士,助理研究员,研究方向:微藻生物技术;E-mail: zhoulin@tib.cas.cn
  • 基金资助:
    天津市合成生物技术创新能力提升行动(TSBICIP-CXRC-027);天津市合成生物技术创新能力提升行动(TSBICIP-IJCP-001);科技部科技伙伴计划(KY202001017)

Biosynthesis of Natural Carotenoids:Progress and Perspective

ZHOU Lin1,2(), LIANG Xuan-ming1, ZHAO Lei1,2()   

  1. 1. Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308
    2. Key Laboratory of Systems Microbial Biotechnology,Chinese Academy of Sciences,Tianjin 300308
  • Received:2021-09-14 Published:2022-07-26 Online:2022-08-09

摘要:

类胡萝卜素是广泛存在于高等植物、微生物和藻类中的一大类天然色素物质的总称,是重要的天然食用色素群之一。细胞工厂被广泛用于各种化学品、食品和药品等的生产,是绿色生物制造的中心环节。利用细胞工厂合成来源稀缺、获取难度大的高附加值天然类胡萝卜素,具有经济可行和环境友好等优势。类胡萝卜素在抗氧化、视力保护和抗癌等方面具有非常重要的作用。本文对生物合成类胡萝卜素细胞工厂构建过程中的类胡萝卜素基本合成路径解析、底盘细胞的选择和改造、途径的模块组装与适配及合成生物学策略等展开详细介绍。同时,对未来类胡萝卜素生物合成所面临的机遇和挑战进行了展望。

关键词: 类胡萝卜素, 虾青素, β-胡萝卜素, 叶黄素, 合成生物学

Abstract:

Carotenoids are the general term for a large class of natural pigment substances broadly in higher plants,microorganisms and algae,one of important natural pigments groups for food. Cell factories are widely used in the production of various chemicals,food and drugs,and are the central link of green biological manufacturing. It is economically feasible and environmentally friendly to synthesize source-scarce,difficult-to-be-obtained and high value-added natural carotenoids in cell factories. Carotenoids play important roles in anti-oxidation,eye-vision protection and anti-cancer. In the present review,the analysis of carotenoid biosynthesis pathway,the selection and modification of chassis cells,the module assembly and adaptation of pathway,and the strategy of synthetic biology during the construction of cell factory used for carotenoids biosynthesis are introduced in details. Future opportunities and challenges in the industrial production of carotenoids are discussed.

Key words: carotenoids, astaxanthin, β-carotene, lutein, synthetic biology