小麦叶锈菌与小麦互作的酵母双杂交cDNA文库构建与应用

doi:10.13560/j.cnki.biotech.bull.1985.2023-0251

生物技术通报 ›› 2023, Vol. 39 ›› Issue (9): 136-146.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0251

小麦叶锈菌与小麦互作的酵母双杂交cDNA文库构建与应用

温晓蕾¹^,²(), 李建嫄³, 李娜⁴, 张娜¹, 杨文香¹()

1.河北农业大学植物保护学院植物病理学系河北省农作物病虫害生物防治技术创新中心国家北方山区农业工程技术研究中心，保定 071001
2.河北科技师范学院农学与生物科技学院河北省作物逆境生物学重点实验室，秦皇岛 066000
3.邢台学院生物科学与工程学院，邢台 054000
4.河北省植保植检总站，石家庄 050000

收稿日期:2023-03-21 出版日期:2023-09-26 发布日期:2023-10-24
通讯作者: 杨文香，女，博士，教授，研究方向：小麦锈病病原菌致病机制；E-mail: wenxiangyang2003@163.com
作者简介:温晓蕾，女，硕士，高级实验师，研究方向：小麦锈病病原菌致病机制；E-mail: xiaoleiwen@sina.com
基金资助:
国家自然科学基金项目(32172367);河北省产业体系小麦创新团队(HCT2018010204)

Construction and Utilization of Yeast Two-hybrid cDNA Library of Wheat Interacted by Puccinia triticina

WEN Xiao-lei¹^,²(), LI Jian-yuan³, LI Na⁴, ZHANG Na¹, YANG Wen-xiang¹()

1. Wheat Leaf Rust Research Center, College of Plant Protection, Agricultural University of Hebei, Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province, National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding 071001
2. College of Agronomy and Biotechnology, Hebei Normal University of Science ＆ Technology, Hebei Key Laboratory of Crop Stress Biology, Qinhuangdao 066000
3. College of Bioscience and Bioengineering, Xingtai University, Xingtai 054000
4. Hebei Plant Protection Plant Inspection Station, Shijiazhuang 050000

Received:2023-03-21 Published:2023-09-26 Online:2023-10-24

摘要/Abstract

摘要：

为筛选小麦叶锈菌效应蛋白的互作靶标蛋白，解析效应蛋白干扰寄主的防御反应机制。本试验以小麦叶锈菌菌株13-5-28-1（JHKT）与感病品种Thatcher在0-12 d互作的供试样本为材料，利用SMART方法构建三框均一化的、用于筛选与小麦叶锈菌互作的小麦cDNA文库，采用同源重组的方法构建效应因子Pt34084重组诱饵载体pGBKT7-Pt34084，并以其为诱饵蛋白，利用共转化方法筛选互作的靶标蛋白。结果表明，构建的小麦叶锈菌与小麦互作的cDNA文库库容约1.05×10⁷ CFU/mL，滴度为1.2×10⁹ CFU/mL，平均插入片段大于1 kb，重组阳性率为100%，符合cDNA建库要求。构建的诱饵重组载体pGBKT7-Pt34084在SD/-Trp-His-Ade培养基中添加20 mmol/L 3-AT及400 ng/mL ABA可抑制其自激活性。利用该文库共筛选获得16个来自小麦及叶锈菌的不同候选互作蛋白，这些蛋白涉及植物代谢、激素信号转导、抗病反应等多个方面。预示Pt34084可通过多种渠道干扰寄主的防御反应，促进叶锈菌侵染。研究结果有助于解析小麦叶锈菌致病机理，为创造新的有效防控小麦叶锈菌策略奠定基础。

关键词: 小麦叶锈菌, 小麦叶锈病, cDNA文库, 酵母双杂交, 效应蛋白, 互作蛋白, 筛选

Abstract:

In order to screen the target proteins interacting with effector proteins of the wheat leaf rust and to lay a foundation for dissecting the defense response mechanism of the effector proteins interfering with hosts and disease resistance breeding, the samples of interaction between the isolate13-5-28-1（JHKT）and susceptible wheat variety Thatcher at 0-12 d were collected. The three-frame homogenization was constructed by SMART method to screen the wheat cDNA library interacting with the wheat leaf rust. The homologous recombination method was used to construct the recombinant bait vector pGBKT7-Pt34084 of the effector factor Pt34084, and it was used as the bait protein to screen the interaction target protein by co-transformation method. The results showed that the cDNA library structure of the interaction between the wheat leaf rust and wheat was successfully constructed. The library capacity was about 1.05×10⁷ CFU/mL, the titer was 1.2×10⁹ CFU/mL, the average insertion fragment was more than 1 kb, and the recombination positive rate was 100%. The cDNA library met the requirement. The constructed bait recombinant vector pGBKT7-Pt34084 inhibited its self-activation after the addition of 20 mmol/L 3-AT and 400 ng/mL ABA to SD/-Trp-His-Ade medium. By this library a total of 16 different candidate interacting proteins from the wheat and the leaf rust were screened. These proteins were involved in plant metabolism, hormone signal transduction, plant disease resistance and other aspects. It indicated that Pt34084 could interfere with the host defense response and promote leaf rust infection through multiple pathways. The results are conducive to analyzing the pathogenic mechanism of wheat leaf rust and lay a foundation for creating new effective strategies to prevent and control wheat leaf rust.

Key words: Puccinia triticina Eriks., wheat leaf rust, cDNA library, yeast two-hybrid, effector protein, interaction protein, screening

温晓蕾, 李建嫄, 李娜, 张娜, 杨文香. 小麦叶锈菌与小麦互作的酵母双杂交cDNA文库构建与应用[J]. 生物技术通报, 2023, 39(9): 136-146.

WEN Xiao-lei, LI Jian-yuan, LI Na, ZHANG Na, YANG Wen-xiang. Construction and Utilization of Yeast Two-hybrid cDNA Library of Wheat Interacted by Puccinia triticina[J]. Biotechnology Bulletin, 2023, 39(9): 136-146.

图/表 11

参考文献 47

[1]	Hovmøller MS, Walter S, Justesen AF. Escalating threat of wheat rusts[J]. Science, 2010, 329(5990): 369. doi: 10.1126/science.1194925 pmid: 20651122
[2]	Huerta-Espino J, Singh RP, Germán S, et al. Global status of wheat leaf rust caused by Puccinia triticina[J]. Euphytica, 2011, 179(1): 143-160. doi: 10.1007/s10681-011-0361-x URL
[3]	Boydom A, Dawit W, Getaneh W. Evaluation of detached leaf assay for assessing leaf rust(Puccinia triticina eriks.) resistance in wheat[J]. J Plant Pathol Microbiol, 2013, 4: 5.
[4]	刘源. 66个小麦品种(系)抗叶锈基因鉴定及Kenya Kudu成株抗叶锈QTL定位[D]. 保定: 河北农业大学, 2020.
	Liu Y. Identification of leaf rust resistance genes of 66 wheat varieties(lines)and QTL mapping of leaf rust resistance in Kenya Kudu adult plants[D]. Baoding: Hebei Agricultural University, 2020.
[5]	Voegele RT, Hahn M, Mendgen K. The Uredinales: cytology, biochemistry, and molecular biology[M]// Plant Relationships. Berlin, Heidelberg: Springer, 2009: 69-98.
[6]	Voegele RT, Mendgen KW. Nutrient uptake in rust fungi: how sweet is parasitic life?[J]. Euphytica, 2011, 179(1): 41-55. doi: 10.1007/s10681-011-0358-5 URL
[7]	Catanzariti AM, Dodds PN, Ellis JG. Avirulence proteins from haustoria-forming pathogens[J]. FEMS Microbiol Lett, 2007, 269(2): 181-188. pmid: 17343675
[8]	Chaudhari P, Ahmed B, Joly DL, et al. Effector biology during biotrophic invasion of plant cells[J]. Virulence, 2014, 5(7): 703-709. doi: 10.4161/viru.29652 pmid: 25513771
[9]	Lewis JD, Guttman DS, Desveaux D. The targeting of plant cellular systems by injected type III effector proteins[J]. Semin Cell Dev Biol, 2009, 20(9): 1055-1063. doi: 10.1016/j.semcdb.2009.06.003 pmid: 19540926
[10]	Vargas WA, Sanz-Martín JM, Rech GE, et al. A fungal effector with host nuclear localization and DNA-binding properties is required for maize anthracnose development[J]. Mol Plant Microbe Interactions, 2016, 29(2): 83-95. doi: 10.1094/MPMI-09-15-0209-R URL
[11]	Win J, Chaparro-Garcia A, Belhaj K, et al. Effector biology of plant-associated organisms: concepts and perspectives[J]. Cold Spring Harb Symp Quant Biol, 2012, 77: 235-247. doi: 10.1101/sqb.2012.77.015933 pmid: 23223409
[12]	McNally KE, Menardo F, Lüthi L, et al. Distinct domains of the AVRPM3A2/F2 avirulence protein from wheat powdery mildew are involved in immune receptor recognition and putative effector function[J]. New Phytol, 2018, 218(2): 681-695. doi: 10.1111/nph.2018.218.issue-2 URL
[13]	Bourras S, Kunz L, Xue MF, et al. The AvrPm3-Pm3 effector-NLR interactions control both race-specific resistance and host-specificity of cereal mildews on wheat[J]. Nat Commun, 2019, 10: 2292. doi: 10.1038/s41467-019-10274-1 pmid: 31123263
[14]	Fitzgerald KA, Kagan JC. Toll-like receptors and the control of immunity[J]. Cell, 2020, 180(6): 1044-1066. doi: S0092-8674(20)30218-X pmid: 32164908
[15]	He Q, McLellan H, Boevink PC, et al. All Roads lead to susceptibility: the many modes of action of fungal and oomycete intracellular effectors[J]. Plant Commun, 2020, 1(4): 100050. doi: 10.1016/j.xplc.2020.100050 URL
[16]	Wang SS, Xing RK, Wang Y, et al. Cleavage of a pathogen apoplastic protein by plant subtilases activates host immunity[J]. New Phytol, 2021, 229(6): 3424-3439. doi: 10.1111/nph.17120 pmid: 33251609
[17]	Liu CH, Pedersen C, Schultz-Larsen T, et al. The stripe rust fungal effector PEC6 suppresses pattern-triggered immunity in a host species-independent manner and interacts with adenosine kinases[J]. New Phytol, 2016: 2016-21278.
[18]	Wang XD, Yang BJ, Li K, et al. A conserved Puccinia striiformis protein interacts with wheat NPR1 and reduces induction of Pathogenesis-Related genes in response to pathogens[J]. Mol Plant Microbe Interactions, 2016, 29(12): 977-989. doi: 10.1094/MPMI-10-16-0207-R URL
[19]	Petre B, Saunders DGO, Sklenar J, et al. Heterologous expression screens in Nicotiana benthamiana identify a candidate effector of the wheat yellow rust pathogen that associates with processing bodies[J]. PLoS One, 2016, 11(2): e0149035. doi: 10.1371/journal.pone.0149035 URL
[20]	Qi T, Guo J, Liu P, et al. Stripe rust effector PstGSRE1 disrupts nuclear localization of ROS-promoting transcription factor TaLOL2 to defeat ROS-induced defense in wheat[J]. Mol Plant, 2019, 12(12): 1624-1638. doi: S1674-2052(19)30328-4 pmid: 31606466
[21]	Yang Q, Huai BY, Lu YX, et al. A stripe rust effector Pst18363 targets and stabilises TaNUDX23 that promotes stripe rust disease[J]. New Phytol, 2020, 225(2): 880-895. doi: 10.1111/nph.16199 pmid: 31529497
[22]	Xu Q, Tang CL, Wang XD, et al. An effector protein of the wheat stripe rust fungus targets chloroplasts and suppresses chloroplast function[J]. Nat Commun, 2019, 10: 5571. doi: 10.1038/s41467-019-13487-6 pmid: 31804478
[23]	Yin CT, Ramachandran SR, Zhai Y, et al. A novel fungal effector from Puccinia graminis suppressing RNA silencing and plant defense responses[J]. New Phytol, 2019, 222(3): 1561-1572. doi: 10.1111/nph.2019.222.issue-3 URL
[24]	Liu C, Wang YQ, Wang YF, et al. Glycine-serine-rich effector PstGSRE4 in Puccinia striiformis f. sp. tritici inhibits the activity of copper zinc superoxide dismutase to modulate immunity in wheat[J]. PLoS Pathog, 2022, 18(7): e1010702. doi: 10.1371/journal.ppat.1010702 URL
[25]	Andres S, William R, Wang SC, et al. Variation in the AvrSr35 gene determines Sr35 resistance against wheat stem rust race Ug99[J]. Science, 2017, 358(6370): 1604-1606. doi: 10.1126/science.aao7294 URL
[26]	Chen JP, Upadhyaya NM, Ortiz D, et al. Loss of AvrSr50 by somatic exchange in stem rust leads to virulence for Sr50 resistance in wheat[J]. Science, 2017, 358(6370): 1607-1610. doi: 10.1126/science.aao4810 URL
[27]	Stephen B, Eduard A, Gitta C, et al. Dissection of cell death induction by wheat stem rust resistance protein Sr35 and its matching effector AvrSr35[J]. Mol Plant Microbe Interact MPMI, 2020, 33(2): 308-319. doi: 10.1094/MPMI-08-19-0216-R URL
[28]	Segovia V, Bruce M, Shoup Rupp JL, et al. Two small secreted proteins from Puccinia triticinainduce reduction of β-glucoronidase transient expression in wheat isolines containing Lr9, Lr24 and Lr26[J]. Can J Plant Pathol, 2016, 38(1): 91-102. doi: 10.1080/07060661.2016.1150884 URL
[29]	齐悦, 吕峻元, 张悦, 等. 小麦叶锈菌效应蛋白Pt18906激发TcLr27+31的双层防御反应[J]. 中国农业科学, 2020, 53(12): 2371-2384. doi: 10.3864/j.issn.0578-1752.2020.12.006
	Qi Y, Lü JY, Zhang Y, et al. Puccinia triticina effector protein Pt18906 triggered two-layer defense reaction in TcLr27+31[J]. Sci Agric Sin, 2020, 53(12): 2371-2384.
[30]	Qi Y, Li JY, Mapuranga J, et al. Wheat leaf rust fungus effector Pt13024 is avirulent to TcLr30[J]. Front Plant Sci, 2023, 13: 1098549. doi: 10.3389/fpls.2022.1098549 URL
[31]	张悦. 小麦叶锈菌效应蛋白Pt2567的特性及功能分析[D]. 保定: 河北农业大学, 2020.
	Zhang Y. Characteristics and functional analysis of wheat leaf rust effector protein Pt2567[D]. Baoding: Hebei Agricultural University, 2020.
[32]	Bi WS, Zhao SQ, Zhao JJ, et al. Rust effector PNPi interacting with wheat TaPR1a attenuates plant defense response[J]. Phytopathol Res, 2020, 2(1): 1-14. doi: 10.1186/s42483-019-0043-5
[33]	李建嫄. 基于转录组分析的小麦叶锈菌效应蛋白的筛选及功能分析[D]. 保定: 河北农业大学, 2018.
	Li JY. Screening and functional analysis of effector proteins of wheat leaf rust based on transcriptome analysis[D]. Baoding: Hebei Agricultural University, 2018.
[34]	郭金菊, 史亮亮, 王茹芳, 等. 苦瓜果实酵母双杂交文库构建及McRPF互作蛋白筛选[J]. 核农学报, 2022, 36(7): 1293-1299. doi: 10.11869/j.issn.100-8551.2022.07.1293
	Guo JJ, Shi LL, Wang RF, et al. Construction of yeast two-hybrid library and screening of McRPF interacting proteins in the fruit of bitter gourd[J]. J Nucl Agric Sci, 2022, 36(7): 1293-1299. doi: 10.11869/j.issn.100-8551.2022.07.1293
[35]	白志英, 王冬梅, 侯春燕, 等. 小麦叶锈菌侵染过程的显微和超微结构[J]. 细胞生物学杂志, 2003(6): 393-397.
	Bai ZY, Wang DM, Hou CY, et al. Microstructure and ultrastructure infected by wheat rust fungus[J]. Chin J Coll Biol, 2003(6): 393-397.
[36]	李舒文, 董笛, 李殷睿智, 等. 蒺藜苜蓿酵母杂交cDNA文库的构建与分析[J]. 分子植物育种, 2021, 19(23): 7861-7866.
	Li SW, Dong D, Li Y, et al. Construction and analysis of a yeast cDNA library from Medicago truncatula[J]. Mol Plant Breed, 2021, 19(23): 7861-7866.
[37]	刘露露, 曲俊杰, 郭泽西, 等. 霜霉菌侵染后葡萄叶片酵母双杂交cDNA文库构建[J]. 南方农业学报, 2020, 51(4): 829-835.
	Liu LL, Qu JJ, Guo ZX, et al. Construction of a yeast two-hybrid cDNA library from Vitis vinifera leaves infected by downy mildew[J]. J South Agric, 2020, 51(4): 829-835.
[38]	王沛雅. 葡萄霜霉菌诱导华东葡萄抗霜霉病基因cDNA文库构建及EST分析[D]. 杨凌: 西北农林科技大学, 2008.
	Wang PY. Construction and EST analysis of cDNA library of downy mildew resistance gene of East China grape induced by downy mildew of grape[D]. Yangling: Northwest A & F University, 2008.
[39]	张引弟, 王荟洁, 陈爱娥, 等. 利用酵母双杂交筛选与致病疫霉菌效应蛋白PITG_07586互作的寄主蛋白[J]. 植物病理学报, 2022, 52(4): 592-600.
	Zhang YD, Wang HJ, Chen AE, et al. Screening of host proteins interacting with effector PITG_07586 of Phytophthora infestans using yeast two-hybrid[J]. Acta Phytopathol Sin, 2022, 52(4): 592-600.
[40]	张凇. 果生炭疽菌效应蛋白CfCE92功能及互作靶标筛选[D]. 杨凌: 西北农林科技大学, 2020.
	Zhang S. Function of effector protein CfCE92 of Colletotrichum gloeosporioides and screening of interaction targets[D]. Yangling: Northwest A & F University, 2020.
[41]	Zhong XT, Li JP, Yang LL, et al. Genome-wide identification and expression analysis of wall-associated kinase(WAK)and WAK-like kinase gene family in response to tomato yellow leaf curl virus infection in Nicotiana benthamiana[J]. BMC Plant Biology, 2023, 23:146. doi: 10.1186/s12870-023-04112-2
[42]	Dou LL, Li ZF, Shen Q, et al. Genome-wide characterization of the WAK gene family and expression analysis under plant hormone treatment in cotton[J]. BMC Genomics, 2021, 22(1): 85. doi: 10.1186/s12864-021-07378-8
[43]	Sivaguru M, Ezaki B, He ZH, et al. Aluminum-induced gene expression and protein localization of a cell wall-associated receptor kinase in Arabidopsis[J]. Plant Physiol, 2003, 132(4): 2256-2266. doi: 10.1104/pp.103.022129 pmid: 12913180
[44]	Lou HQ, Fan W, Jin JF, et al. A NAC-type transcription factor confers aluminum resistance by regulating cell wall-associated receptor kinase 1 and cell wall pectin[J]. Plant Cell Environ, 2020, 43(2): 463-478. doi: 10.1111/pce.v43.2 URL
[45]	程鹏, 徐鹏飞, 范素杰, 等. 野生大豆接种大豆疫霉根腐病菌后过氧化物酶(POD)活性变化[J]. 大豆科学, 2013, 32(2): 197-201.
	Cheng P, Xu PF, Fan SJ, et al. Response of POD activity in Glycine soja inoculated by Phytophthora sojae[J]. Soybean Sci, 2013, 32(2): 197-201.
[46]	阎爱华, 张立峰, 张韫玮, 等. 小麦单基因系TcLr19抗叶锈防卫反应的表达与叶锈菌侵染进程关系的初步研究[J]. 华北农学报, 2013, 28(1): 221-226. doi: 10.3969/j.issn.1000-7091.2013.01.039
	Yan AH, Zhang LF, Zhang YW, et al. Relationship between the activity changes of defense enzymes of wheat near isogenic line TcLr19 and the infection process of Puccinia triticina[J]. Acta Agric Boreali Sin, 2013, 28(1): 221-226.
[47]	Hemetsberger C, Herrberger C, Zechmann B, et al. The Ustilago maydis effector Pep1 suppresses plant immunity by inhibition of host peroxidase activity[J]. PLoS Pathog, 2012, 8(5): e1002684. doi: 10.1371/journal.ppat.1002684 URL

编号No.	基因登录号 GenBank No.	候选蛋白 Candidate proteins	来源 Source	一致性 Identity/%
1	XM_044577317.1	光照后叶绿素荧光增加蛋白，类叶绿体 Post-illumination chlorophyll fluorescence increasing protein, chloroplast	小麦 T. aestivum	100.00
2	XM_037619534.1	光系统I组装蛋白2，类叶绿体 Protein photosystem I assembly 2, chloroplast	野生型二粒小麦 T. dicoccoides	99.61
3	XM_044569896.1	过氧化物酶11 Peroxidase 11-like	小麦 T. aestivum	89.11
4	XM_044551498.1	细胞壁连接的类受体激酶 Wall-associated receptor kinase 2-like	小麦 T. aestivum	88.27
5	XM_044521323.1	蛋白酶体组装伴侣4 Proteasome assembly chaperone 4-like	小麦 T. aestivum	99.02
6	XM_044485885.1	富含丝氨酸的无特征蛋白 Uncharacterized serine-rich protein	小麦 T. aestivum	99.35
7	XM_044514591.1	无特征蛋白 Uncharacterized protein LOC123092769	小麦 T. aestivum	99.47
8	XM_037564333.1	无特征蛋白 Uncharacterized protein LOC119285117	野生型二粒小麦 T. dicoccoides	98.90
9	XM_020296657.3	蛋白磷酸酶2C 26 Protein phosphatase 2C 26	山羊草 Aegilops tauschii	99.40
10	XM_003333436.2	假定蛋白 Hypothetical protein	小麦秆锈菌 P. graminis f. sp. tritici	85.12
11	XM_037577936.1	60S 核糖体蛋白 L15-2 60S ribosomal protein L15-2	野生型二粒小麦 T. dicoccoides	99.72
12	XM_020343972.3	无特征蛋白 Uncharacterized protein LOC109785374	山羊草 A. tauschii	99.13
13	XM_020323127.3	E3泛素蛋白连接酶 XBOS34 Probable E3 ubiquitin-protein ligase XBOS34	山羊草 A. tauschii	99.75
14	XM_044474326.1	无特征蛋白 Uncharacterized protein LOC123051461	小麦 T. aestivum	93.80
15	XM_003323195.2	甲硫氨酰氨基肽酶 Methionyl aminopeptidase	小麦秆锈菌 P. graminis f. sp. tritici	88.90
16	XM_044559490.1	含卤酸脱卤酶类水解酶结构域的蛋白质 Haloacid dehalogenase-like hydrolase domain-containing protein At2g33255	小麦 T. aestivum	98.92

编号No.	基因登录号 GenBank No.	候选蛋白 Candidate proteins	来源 Source	一致性 Identity/%
1	XM_044577317.1	光照后叶绿素荧光增加蛋白，类叶绿体 Post-illumination chlorophyll fluorescence increasing protein, chloroplast	小麦 T. aestivum	100.00
2	XM_037619534.1	光系统I组装蛋白2，类叶绿体 Protein photosystem I assembly 2, chloroplast	野生型二粒小麦 T. dicoccoides	99.61
3	XM_044569896.1	过氧化物酶11 Peroxidase 11-like	小麦 T. aestivum	89.11
4	XM_044551498.1	细胞壁连接的类受体激酶 Wall-associated receptor kinase 2-like	小麦 T. aestivum	88.27
5	XM_044521323.1	蛋白酶体组装伴侣4 Proteasome assembly chaperone 4-like	小麦 T. aestivum	99.02
6	XM_044485885.1	富含丝氨酸的无特征蛋白 Uncharacterized serine-rich protein	小麦 T. aestivum	99.35
7	XM_044514591.1	无特征蛋白 Uncharacterized protein LOC123092769	小麦 T. aestivum	99.47
8	XM_037564333.1	无特征蛋白 Uncharacterized protein LOC119285117	野生型二粒小麦 T. dicoccoides	98.90
9	XM_020296657.3	蛋白磷酸酶2C 26 Protein phosphatase 2C 26	山羊草 Aegilops tauschii	99.40
10	XM_003333436.2	假定蛋白 Hypothetical protein	小麦秆锈菌 P. graminis f. sp. tritici	85.12
11	XM_037577936.1	60S 核糖体蛋白 L15-2 60S ribosomal protein L15-2	野生型二粒小麦 T. dicoccoides	99.72
12	XM_020343972.3	无特征蛋白 Uncharacterized protein LOC109785374	山羊草 A. tauschii	99.13
13	XM_020323127.3	E3泛素蛋白连接酶 XBOS34 Probable E3 ubiquitin-protein ligase XBOS34	山羊草 A. tauschii	99.75
14	XM_044474326.1	无特征蛋白 Uncharacterized protein LOC123051461	小麦 T. aestivum	93.80
15	XM_003323195.2	甲硫氨酰氨基肽酶 Methionyl aminopeptidase	小麦秆锈菌 P. graminis f. sp. tritici	88.90
16	XM_044559490.1	含卤酸脱卤酶类水解酶结构域的蛋白质 Haloacid dehalogenase-like hydrolase domain-containing protein At2g33255	小麦 T. aestivum	98.92

小麦叶锈菌与小麦互作的酵母双杂交cDNA文库构建与应用

Construction and Utilization of Yeast Two-hybrid cDNA Library of Wheat Interacted by Puccinia triticina

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 11

参考文献 47

相关文章 15

编辑推荐

Metrics

本文评价

组分 Component	浓度 Concentration
3-AT/（mmol·L^-1）	0	10	20	30	40
ABA/（ng·mL^-1）	0	200	400	600	800

[1]	王子颖, 龙晨洁, 范兆宇, 张蕾. 利用酵母双杂交系统筛选水稻中与OsCRK5互作蛋白[J]. 生物技术通报, 2023, 39(9): 117-125.
[2]	吴巧茵, 施友志, 李林林, 彭政, 谭再钰, 刘利平, 张娟, 潘勇. 类胡萝卜素降解菌株的原位筛选及其在雪茄提质增香中的应用[J]. 生物技术通报, 2023, 39(9): 192-201.
[3]	韩浩章, 张丽华, 李素华, 赵荣, 王芳, 王晓立. 盐碱胁迫诱导的猴樟酵母cDNA文库构建及CbP5CS上游调控因子筛选[J]. 生物技术通报, 2023, 39(9): 236-245.
[4]	江海溶, 崔若琪, 王玥, 白淼, 张明露, 任连海. NH₃和H₂S降解功能菌的分离鉴定及降解特性研究[J]. 生物技术通报, 2023, 39(9): 246-254.
[5]	薛宁, 王瑾, 李世新, 刘叶, 程海娇, 张玥, 毛雨丰, 王猛. 多基因同步调控结合高通量筛选构建高产L-苯丙氨酸的谷氨酸棒杆菌工程菌株[J]. 生物技术通报, 2023, 39(9): 268-280.
[6]	马俊秀, 吴皓琼, 姜威, 闫更轩, 胡基华, 张淑梅. 蔬菜软腐病菌广谱拮抗细菌菌株筛选鉴定及防效研究[J]. 生物技术通报, 2023, 39(7): 228-240.
[7]	谢东, 汪流伟, 李宁健, 李泽霖, 徐子航, 张庆华. 一株多功能菌株的发掘、鉴定及解磷条件优化[J]. 生物技术通报, 2023, 39(7): 241-253.
[8]	李典典, 粟元, 李洁, 许文涛, 朱龙佼. 抗菌适配体的筛选与应用进展[J]. 生物技术通报, 2023, 39(6): 126-132.
[9]	董聪, 高庆华, 王玥, 罗同阳, 王庆庆. 基于联合策略提高FAD依赖的葡萄糖脱氢酶的酵母表达[J]. 生物技术通报, 2023, 39(6): 316-324.
[10]	王艺清, 王涛, 韦朝领, 戴浩民, 曹士先, 孙威江, 曾雯. 茶树SMAS基因家族的鉴定及互作分析[J]. 生物技术通报, 2023, 39(4): 246-258.
[11]	侯筱媛, 车郑郑, 李姮静, 杜崇玉, 胥倩, 王群青. 大豆膜系统cDNA文库的构建及大豆疫霉效应子PsAvr3a互作蛋白的筛选[J]. 生物技术通报, 2023, 39(4): 268-276.
[12]	李天顺, 李宸葳, 王佳, 朱龙佼, 许文涛. 功能核酸筛选过程中次级文库的有效制备[J]. 生物技术通报, 2023, 39(3): 116-122.
[13]	王涛, 漆思雨, 韦朝领, 王艺清, 戴浩民, 周喆, 曹士先, 曾雯, 孙威江. CsPPR和CsCPN60-like在茶树白化叶片中的表达分析及互作蛋白验证[J]. 生物技术通报, 2023, 39(3): 218-231.
[14]	扈丽丽, 林柏荣, 王宏洪, 陈建松, 廖金铃, 卓侃. 最短尾短体线虫转录组及潜在效应蛋白分析[J]. 生物技术通报, 2023, 39(3): 254-266.
[15]	崔若琪, 张玲悦, 江海溶, 张毓羚, 张明露, 任连海. NH₃和H₂S除臭菌剂的制备及其对厨余垃圾堆肥除臭效果和机理探究[J]. 生物技术通报, 2023, 39(10): 311-322.