一株产阿魏酸酯酶丝状真菌的分离鉴定及其粗酶性质研究

生物技术通报 ›› 2014, Vol. 0 ›› Issue (7): 162-167.

一株产阿魏酸酯酶丝状真菌的分离鉴定及其粗酶性质研究

胡泓宇, 周燕燕, 陈静, 侯运华

齐鲁工业大学食品与生物工程学院, 济南 250353

收稿日期:2013-12-30 出版日期:2014-07-15 发布日期:2014-07-16
作者简介:胡泓宇, 女, 硕士研究生, 研究方向：丝状真菌发酵和分子生物学；E-mail：929112837@qq.com
基金资助:
山东省高等学校科技计划项目（J10LC08）

Isolation, Identification and Enzyme Properties of a Strain Producing Feruloyl Esterases

Hu Hongyu, Zhou Yanyan, Chen Jing, Hou Yunhua

Collage of Food and bioengineering, Qilu University of Technology, Jinan 250353

Received:2013-12-30 Published:2014-07-15 Online:2014-07-16

摘要/Abstract

摘要： 利用平板透明圈法, 筛选分离到37株具有阿魏酸酯酶活性的丝状真菌, 其中1株编号为HA4087的菌株具有较强的产阿魏酸酯酶能力。经形态学观察、18S rDNA和ITS序列鉴定为互隔交链格孢霉。对该菌株所产阿魏酸酯酶的粗酶性质进行了初步研究, 结果表明, 在发酵培养基中发酵粗酶活力为86 mU/mL, 最适作用温度为55℃, 最适pH值为5.5；在55℃保温30 min后酶活力仍为90%以上；在pH4.5-6.5范围内稳定性较好, 酶活仍为85%以上。试验获得了产高酶活阿魏酸酯酶的菌株, 为阿魏酸酯酶的工业化应用提供前提。

关键词: 阿魏酸酯酶, 菌种鉴定, 互隔交链格孢霉, 酶学性质

Abstract: Using the method of transparent zone, 37 filamentous fungi producing feruloyl esterase（FAE）were selected from rot soil samples containing wood fibers. The strain HA4087 was isolated by its high FAE activities. It was identified as Alternaria alternata by conidia morphology, 18S rDNA sequence and ITS sequence analysis. The characteristics of crude feruloyl esterase from A. alternate HA4087 were preliminarily studied. FAE activity of the crude enzyme was 86 mU/mL. The optimal temperature and pH for crude FAE was 55℃ and pH5.5, respectively. Crude FAE from A. alternate HA4087 remained nearly 90% of its maximal activity at 70℃ and was active in the pH range of 3.0-7.0. The strain producing thermostable and high active FAE provided a prerequisite for the industrialization of feruloyl esterase.

Key words: Feruloyl esterase, Identification, Alternaria alternata, Enzyme properties

胡泓宇, 周燕燕, 陈静, 侯运华. 一株产阿魏酸酯酶丝状真菌的分离鉴定及其粗酶性质研究[J]. 生物技术通报, 2014, 0(7): 162-167.

Hu Hongyu, Zhou Yanyan, Chen Jing, Hou Yunhua. Isolation, Identification and Enzyme Properties of a Strain Producing Feruloyl Esterases[J]. Biotechnology Bulletin, 2014, 0(7): 162-167.

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