关键词: 高山被孢霉, 基因组, 转录组, 提取技术

Abstract: High-throughput sequencing of transcriptome and genome demands the high purity and content of sample,the purpose of this work is to determine the optimal extraction technology by studying the effects of Mortierella alpina in different culture conditions and using different hypha processing methods on the genome and transcriptome quality. The results showed that the mycelium from liquid fermentation medium after genome extraction presented a significant difference in agarose gel electrophoresis compared to the mycelium from PDA culture medium,the former gave degradation-free genome DNA but the latter was extracted genomic DNA of some degradation. During using domestic brand kit to study M. alpina tanscriptome RNA extraction,it was found that using liquid nitrogen fast-frozen method to break the hypha cell wall combined with applying β-Mercaptoethanol resulted in more likely to extract the high concentration and no-degradation transcription sample.

Key words: Mortierella alpina, genome, transcriptome, extraction technology