转录组-代谢组分析方法及其在药物作用机理研究中的应用

doi:10.13560/j.cnki.biotech.bull.1985.2018-0497

摘要/Abstract

摘要： 随着高通量测序技术的发展,目前高通量整合分析技术是获取最终生物学信息必不可少的重要手段,是对传统药物作用机理研究方法的一次革命性变革。转录组学、代谢组学及二者联合应用是系统生物学的重要组成部分,近年来广泛应用在药物作用机理研究相关的各个领域,如新药开发、提高药效和评价药物毒性、指导药物联合治疗等方面,已成为研究药物作用机理中不可或缺的筛选阶段。同时对转录组学、代谢组学、四种转录组-代谢组联合分析方法进行了综述,对不同联合分析方法的优缺点及存在的问题进行简要分析,阐述了近几年两组学联合分析方法在药物作用机理研究中的应用,展望其下一步的发展前景与挑战,以期探讨转录组学和代谢组学及其二者联合应用在药物作用机制研究中的策略,为今后药物作用的分子机理研究提供借鉴与参考,进而基于现有研究基础发掘新的研究方法与途径。

关键词: 转录组学, 代谢组学, 转录组-代谢组联合分析方法, 药物作用机理

Abstract: With the development of high-throughput sequencing technology,high-throughput integrated analysis technology is an essential means to obtain the final biological information,and it is a revolutionary change to the research method for traditional drug action mechanism. Transcriptomics,metabolomics and the use of their combination are important components of systems biology. In recent years,they have been widely used in various fields related to drug mechanism research,such as development of new drug,improvement of drug efficacy,evaluating drug toxicity,and guiding therapy by combined drugs,etc.,it has become an indispensable screening stage in studying the drug action mechanisms. Here we review transcriptomics,metabolomics and 4 transcriptome-metabolomics conjoint analysis methods,analyze the advantages and disadvantages of the different methods as well as the existing problems,and briefly describe the application of the combined analysis method in the study of the drug action mechanism in recent years. Concurrently,we forecast its next development and challenges,with a view to exploring the strategies of transcriptomics and metabolomics and their combination in studying drug action mechanisms for future drugs. We expect that this review may provide the reference for understanding the molecular mechanism of drug action and subsequently for exploring new research methods and approaches based on the existing research foundation.

Key words: transcriptomics, metabolomics, transcriptome-metabolism conjoint analysis method, drug action mechanism

金玉, 李赫健, 冯成强. 转录组-代谢组分析方法及其在药物作用机理研究中的应用[J]. 生物技术通报, 2018, 34(12): 68-76.

JIN Yu, LI He-jian, FENG Cheng-qiang. Transcriptome-metabolomics Analysis and Its Application in Studying Drug Action Mechanism[J]. Biotechnology Bulletin, 2018, 34(12): 68-76.

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