基于三种碳源筛选低温纤维素降解菌及其复合系的降解能力分析

doi:10.13560/j.cnki.biotech.bull.1985.2018-0956

摘要/Abstract

摘要： 分别以微晶纤维素、羧甲基纤维素（Carboxymethylcellulose,CMC）和D-水杨苷三种不同碳源在4℃下对呼伦贝尔森林土壤中的低温纤维素降解细菌进行筛选,将酶活性高的菌株分别混合构建低温降解复合系并测定其滤纸降解能力。结果共分离到172株低温纤维素降解细菌,以微晶纤维素、CMC为碳源分离到的细菌均分属于4个纲,以D-水杨苷为碳源分离到的细菌分属于6个纲,第一优势菌纲均为γ-Proteobacteria,比例分别为54%、48%和55%,其次为α-Proteobacteria,比例分别为28%、26%和34%;第一优势菌属均为Pseudomonas,比例分别为35%、26%和26%,以微晶纤维素为碳源分离到的细菌第二优势菌属为Rhizobium,比例为15%,以CMC为碳源筛到的细菌第二优势菌属为Rhizobium 和Oerskovia,比例为12%,以D-水杨苷为碳源分离到的细菌第二优势菌属为Lelliottia比例为19%。构建的27个低温降解复合系中滤纸酶活性最高的降解复合系为组合D13'1"和D13'2",酶活性可达158.02 U/mL,是其复合系中单菌株的5-10倍,为低温降解复合系最优菌群组合,具有较高的应用潜力。

关键词: 低温, 纤维素降解菌, 复合系, 滤纸酶活, 碳源

Abstract: The cold-adapted cellulose-degrading bacteria were screened from the forest soil of Hulun Buir city using avicel,carboxymethylcellulose（CMC）and D-salicin as carbon source respectively at 4℃. The strains of high enzyme activity were respectively mixed to construct cold-adapted cellulose-degrading consortia and their filter paper degradation abilities were measured. As a result,172 strains of cold-adapted cellulose-degrading bacteria were isolated. Of them,the bacteria isolated with avicel or CMC as carbon source belonged to 4 classes,and the bacteria isolated with D-salicin as carbon source belonged to 6 classes. The first dominant class was γ-Proteobacteria with ratio of 54%,48% and 55%,respectively;the second dominant class was α-Proteobacteria with ratio of 28%,26% and 34% respectively. The first dominant genus was Pseudomonaswith ratio of 35%,26% and 26% respectively,the second dominant genus screened with avicel as carbon source was Rhizobium with ratio of 15%,the second dominant genera screened with CMC as carbon source were Rhizobium and Oerskasty with ratio of 12%,the second dominant genus screened with D-salicin as carbon source was Lelliottia with ratio of 19%. In 27 cold-adapted cellulose-degrading consortia,D13'1'' and D13'2'' presented the highest filter paper enzyme activity of 158.02 U/mL,which was 5-10 times more than that of any single strain in its consortia. D13'1'' and D13'2'' are the optimal flora combination of cold-adapted cellulose-degrading consortia with high application potential.

Key words: cold-adapted, cellulose-degrading bacteria, consortia, filter paper enzyme activity, carbon source

孟建宇, 冀锦华, 贾丽娟, 郭慧琴, 陶羽, 冯福应. 基于三种碳源筛选低温纤维素降解菌及其复合系的降解能力分析[J]. 生物技术通报, 2019, 35(8): 77-84.

MENG Jian-yu, JI Jin-hua, JIA Li-juan, GUO Hui-qin, TAO Yu, FENG Fu-ying. Isolation of Cold-adapted Cellulose-degrading Bacteria Using Three Different Carbon Sources and Analysis on the Degrading Ability of Consortia[J]. Biotechnology Bulletin, 2019, 35(8): 77-84.

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