炎症与肿瘤发生对造血祖细胞发育失衡的影响

doi:10.13560/j.cnki.biotech.bull.1985.2018-1008

生物技术通报 ›› 2019, Vol. 35 ›› Issue (8): 155-161.doi: 10.13560/j.cnki.biotech.bull.1985.2018-1008

炎症与肿瘤发生对造血祖细胞发育失衡的影响

李晓玉¹, 刘霖¹, 邢兵¹, 汤婧¹, 刘亚平¹, 周祖平^2,3, 蒲仕明^1,2,3

1. 广西师范大学生命科学学院,桂林 541004;
2. 广西高校干细胞与医药生物技术重点实验室,桂林 541004;
3. 广西师范大学生物医学;研究中心,桂林 541004

收稿日期:2018-11-23 出版日期:2019-08-26 发布日期:2019-08-05
作者简介:李晓玉,男,硕士研究生,研究方向：细胞生物学；E-mail：lixiaoyu2016@foxmial.com
基金资助:
国家自然科学基金项目（31270949）,广西省自然科学基金项目（2018GXNSFBA281115）,广西研究生教育创新计划项目（YCSW2018097,XYCSZ2018058）

Effects of Inflammation and Tumorigenesis on the Imbalance of Hematopoietic Progenitor Cells

LI Xiao-yu¹, LIU Lin¹, XING Bing¹, TANG Jing¹, LIU Ya-ping¹, ZHOU Zu-ping^2,3, PU Shi-ming^1,2,3

1. College of Life Sciences,Guangxi Normal University,Guilin 541004;
2. Guangxi Universities Key Laboratory of Stem Cell and Biopharmaceutical Technology,Guilin 541004;
3. Biomedical Research Center,Guangxi Normal University,Guilin 541004

Received:2018-11-23 Published:2019-08-26 Online:2019-08-05

摘要/Abstract

摘要： 主要研究炎症和肿瘤对小鼠造血免疫系统中造血祖细胞（Hematopoietic progenitor cells,HPCs）影响,探索炎症和肿瘤状态下造血-免疫系统间的相关性。利用偶氮甲烷（Azoxymethane,AOM）和右旋糖酐硫酸钠（Dextran sulfate sodium salt,DSS）共同诱导构建小鼠炎症性结肠癌模型,并采用流式细胞术分析正常、炎症和肿瘤小鼠造血祖细胞丰度以及细胞周期。炎症和肿瘤进程中,小鼠骨髓中较上游的多能祖细胞（Multi-potent progenitors,MPPs）占骨髓细胞的百分比显著升高,共髓系同祖细胞（Common myeloid progenitors,CMPs）、巨核细胞/ 红系祖细胞（Megakaryocyte/erythrocyte progenitors,MEPs）和粒细胞/巨噬细胞祖细胞（Granulocyte/macrophage progenitors,GMPs）也有类似变化,相反共同淋巴祖细胞（Common lymphoid progenitors,CLPs）则显著下降;并且,炎症和肿瘤状态下,髓系祖细胞分裂能力显著强于正常生理状态;肿瘤状态下,小鼠外周血CD4⁺/CD8⁺T细胞比值显著下降。炎症和肿瘤进程中,HSPCs偏向髓系分化;肿瘤状态下小鼠细胞免疫功能失衡且长期的炎症损害可能会引起免疫系统的失衡并最终导致肿瘤的形成。

关键词: 炎症, 肿瘤, 免疫系统, 免疫功能

Abstract: This article mainly investigates the effects of inflammation and tumors on hematopoietic progenitor cells（HPCs）in the hematopoietic-immune system of mice,and explores the correlation between hematopoietic-immune lines in inflammation and tumor status. AOM（Azoxymethane）and DSS（Dextran sulfate sodium salt）were used to induce the mouse model of inflammatory colon cancer,and flow cytometry was used to the abundance and cell cycle of hematopoietic progenitor cells in normal,inflammatory and tumor mice. In the process of inflammation and tumor progression,the percentage of multi-potent progenitors（MPPs）in the bone marrow of mice significantly increased,and common myeloid progenitors（CMPs）and megakaryocytes/megakaryocyte/erythrocyte progenitors（MEPs）and granulocyte/macrophage progenitors（GMPs）demonstrated similar changes,whereas common lymphoid progenitors（CLPs）decreased significantly. Moreover,in the inflammatory and tumor states,the division ability of myeloid progenitor cell was significantly stronger than the normal physiological state. In addition,the tumor peripheral blood CD4⁺/CD8⁺ T cell ratio decreased significantly in the tumor state. In the process of inflammation and tumor progression,HSPCs were biased toward myeloid differentiation. In the tumor state,the cellular immune function of mice was unbalanced;in addition,long-term inflammatory damage may cause imbalance of the immune system and eventually lead to tumor formation.

Key words: inflammatory, tumor, immune system, immune function

李晓玉, 刘霖, 邢兵, 汤婧, 刘亚平, 周祖平, 蒲仕明. 炎症与肿瘤发生对造血祖细胞发育失衡的影响[J]. 生物技术通报, 2019, 35(8): 155-161.

LI Xiao-yu, LIU Lin, XING Bing, TANG Jing, LIU Ya-ping, ZHOU Zu-ping, PU Shi-ming. Effects of Inflammation and Tumorigenesis on the Imbalance of Hematopoietic Progenitor Cells[J]. Biotechnology Bulletin, 2019, 35(8): 155-161.

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炎症与肿瘤发生对造血祖细胞发育失衡的影响

Effects of Inflammation and Tumorigenesis on the Imbalance of Hematopoietic Progenitor Cells

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