基于CRISPR/Cas系统的生物传感策略研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2019-0932

生物技术通报 ›› 2020, Vol. 36 ›› Issue (3): 69-77.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0932

• 基因编辑专题（专题主编谷峰研究员） • 上一篇下一篇

基于CRISPR/Cas系统的生物传感策略研究进展

胡孝林¹, 王良婷¹, 顾玮², 罗阳²

1. 重庆大学生物工程学院,重庆 400044;
2. 重庆大学医学院,重庆 400044

收稿日期:2019-09-30 出版日期:2020-03-26 发布日期:2020-03-17
作者简介:胡孝林,男,硕士研究生,研究方向：疾病早期检测、分子诊断;E-mail：huxl199604@163.com
基金资助:
国家自然科学基金面上项目（81871733,81572079,81772061）,重庆市技术创新与应用示范（社会民生类）项目（CSCT2018JSCX-MSYBX0146）,重庆大学中央高校基本科研业务专项（2018CDGFSG0017,2019CDYGZD005,2019CDYGZD007,2019CDXYSG0004）

Research Progress of Biosensing Strategy Based on CRISPR/Cas System

HU Xiao-lin¹, WANG Liang-ting¹, GU Wei², LUO Yang²

1. Bioengineering College of Chongqing University,Chongqing 400044;
2. Medical College of Chongqing University,Chongqing 400044

Received:2019-09-30 Published:2020-03-26 Online:2020-03-17

摘要/Abstract

摘要： CRISPR/Cas9系统是继锌指核酸内切酶、类转录激活因子效应物核酸酶之后的第三代基因组定点编辑工具,因其具有特异性切割双链DNA的能力,被广泛应用于基因编辑、生物传感等领域。Cas12a（Cpf1）、Cas13a（C2c2）等蛋白“附属切割”活性的发现,拓展了CRISPR/Cas系统在生物传感中的应用。近年来,研究人员开发出一系列快速、超敏、高特异性的生物传感系统用于分子检测,如SHERLOCK,DETECTR等。本文主要综述了基于CRISPR/Cas系统的生物传感策略的研究进展,并展望了其未来发展的方向。

关键词: CRISPR/Cas系统, 等温扩增, 生物传感, 超敏检测, 单碱基特异性

Abstract: CRISPR/Cas9 system is the third generation of genome editing tools after zinc finger endonuclease and transcription activator-like effector nuclease. Because of its ability to cut double-stranded DNA,CRISPR/Cas9 system is widely used in gene editing and biosensing. With the “collateral cleavage” activity of Cas12a（Cpf1）and Cas13a（C2c2）proteins discovered,the application of CRISPR / CAS system in biosensor has been expanded. In recent years,researchers have developed a series of fast,ultrasensitive and highly specific biosensor for molecular detection,such as SHERLOCK and DETECTR. This review mainly reports the research progress of new biosensors based on CRISPR/CAS system,and prospects its future development.

Key words: CRISPR/Cas system, isothermal amplification, biosensing, ultrasensitive detection, single base specificity

胡孝林, 王良婷, 顾玮, 罗阳. 基于CRISPR/Cas系统的生物传感策略研究进展[J]. 生物技术通报, 2020, 36(3): 69-77.

HU Xiao-lin, WANG Liang-ting, GU Wei, LUO Yang. Research Progress of Biosensing Strategy Based on CRISPR/Cas System[J]. Biotechnology Bulletin, 2020, 36(3): 69-77.

参考文献

[1] Makarova KS, Wolf YI, Alkhnbashi OS, et al.An updated evolutionary classification of CRISPR-Cas systems[J]. Nat Rev Microbiol, 2015, 13(11):722-736.
[2] Hsu PD, Lander ES, Zhang F.Development and applications of CRISPR-Cas9 for genome engineering[J]. Cell, 2014, 157(6):1262-1278.
[3] Deltcheva E, Chylinski K, Sharma CM, et al.CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III[J]. Nature, 2011, 471(7340):602-607.
[4] Shmakov S, Smargon A, Scott D, et al.Diversity and evolution of class 2 CRISPR-Cas systems[J]. Nature Reviews Microbiology, 2017, 15(3):169-182.
[5] Roth TL, Puig-Saus C, Yu R, et al.Reprogramming human T cell function and specificity with non-viral genome targeting[J]. Nature, 2018, 559(7714):405-409.
[6] Haapaniemi E, Botla S, Persson J, et al.CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response[J]. Nat Med, 2018, 24(7):927-930.
[7] Wang M, Sintim HO.Discriminating cyclic from linear nucleotides - CRISPR/Cas-related cyclic hexaadenosine monophosphate as a case study[J]. Anal Biochem, 2019, 567:21-26.
[8] Adli M.The CRISPR tool kit for genome editing and beyond[J]. Nat Commun, 2018, 9(1):1911.
[9] Jinek M, Chylinski K, Fonfara I, et al.A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity[J]. Science, 2012, 337(6096):816-821.
[10] Gasiunas G, Barrangou R, Horvath P, et al.Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria[J]. Proc Natl Acad Sci USA, 2012, 109(39):E2579-E2686.
[11] Gilbert LA, Larson MH, Morsut L, et al.CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes[J]. Cell, 2013, 154(2):442-451.
[12] Zhang Y, Qian L, Wei W, et al.Paired design of dCas9 as a systematic platform for the detection of featured nucleic acid sequences in pathogenic strains[J]. Acs Synth Biol, 2017, 6(2):211-216.
[13] Qiu XY, Zhu LY, Zhu CS, et al.Highly effective and low-cost microRNA detection with CRISPR-Cas9[J]. Acs Synth Biol, 2018, 7(3):807-813.
[14] Zetsche B, Gootenberg JS, Abudayyeh OO, et al.Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-cas system[J]. Cell, 2015, 163(3):759-771.
[15] Chylinski K, Le Rhun A, Charpentier E.The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems[J]. Rna Biol, 2013, 10(5):726-737.
[16] Chen JS, Ma E, Harrington LB, et al.CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity[J]. Science, 2018, 360(6387):436-439.
[17] Shea MJ, King DL, Conboy MJ, et al.Proteins that bind to drosophila chorion cis-regulatory elements:a new C2H2 zinc finger protein and a C2C2 steroid receptor-like component[J]. Genes Dev, 1990, 4(7):1128-1140.
[18] Abudayyeh OO, Gootenberg JS, Konermann S, et al. C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector[J]. Science, 2016, 353(6299):aaf5573.
[19] Zhou W, Hu L, Ying L, et al.A CRISPR-Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection[J]. Nat Commun, 2018, 9(1):5012.
[20] Huang M, Zhou X, Wang H, et al.CRISPR/Cas9 triggered isothermal amplification for site-specific nucleic acid detection[J]. Anal Chem, 2018, 90(3):2193-2200.
[21] Li SY, Cheng QX, Wang JM, et al.CRISPR-Cas12a-assisted nucleic acid detection[J]. Cell Discov, 2018, 4:20.
[22] Li L, Li S, Wu N, et al.HOLMESv2:A CRISPR-Cas12b-assisted platform for nucleic acid detection and DNA methylation quantitation[J]. Acs Synth Biol, 2019, 8(10):2228-2237.
[23] Wang B, Wang R, Wang D, et al.Cas12aVDet:A CRISPR/Cas12a-based platform for rapid and visual nucleic acid detection[J]. Anal Chem, 2019, 91(19):12156-12161.
[24] Hajian R, Balderston S, Tran T, et al.Detection of unamplified target genes via CRISPR-Cas9 immobilized on a graphene field-effect transistor[J]. Nat Biomed Eng, 2019, 3(6):427-437.
[25] Shao N, Han X, Song Y, et al.CRISPR-Cas12a coupled with platinum nanoreporter for visual quantification of SNVs on a volumetric bar-chart chip[J]. Anal Chem, 2019, 91(19):12384-12391.
[26] Teng F, Guo L, Cui T, et al.CDetection:CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity[J]. Genome Biol, 2019, 20(1):132.
[27] Harrington LB, Burstein D, Chen JS, et al.Programmed DNA destruction by miniature CRISPR-Cas14 enzymes[J]. Science, 2018, 362(6416):839-842.
[28] Pardee K, Green AA, Takahashi MK, et al.Rapid, low-cost detection of zika virus using programmable biomolecular components[J]. Cell, 2016, 165(5):1255-1266.
[29] Gootenberg JS, Abudayyeh OO, Lee JW, et al.Nucleic acid detection with CRISPR-Cas13a/C2c2[J]. Science, 2017, 356(6336):438-442.
[30] Gootenberg JS, Abudayyeh OO, Kellner MJ, et al.Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6[J]. Science, 2018, 360(6387):439-444.
[31] Myhrvold C, Freije CA, Gootenberg JS, et al.Field-deployable viral diagnostics using CRISPR-Cas13[J]. Science, 2018, 360(6387):444-448.
[32] Qing GC, Gong NQ, Chen XH, et al.Natural and engineered bacterial outer membrane vesicles[J]. Biophysics Reports, 2019, 5(4):184-198.
[33] Qiu JH, Xu JX, Zhang K, et al.Refining cancer management using integrated liquid biopsy[J]. Theranostics, 2020, 10(5):2374-2384.
[34] Li Y, Teng X, Zhang K, et al.RNA strand displacement responsive CRISPR/Cas9 system for mRNA sensing[J]. Anal Chem, 2019, 91(6):3989-3996.
[35] Liang M, Li Z, Wang W, et al.A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules[J]. Nat Commun, 2019, 10(1):3672.

基于CRISPR/Cas系统的生物传感策略研究进展

Research Progress of Biosensing Strategy Based on CRISPR/Cas System

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	薛宁, 王瑾, 李世新, 刘叶, 程海娇, 张玥, 毛雨丰, 王猛. 多基因同步调控结合高通量筛选构建高产L-苯丙氨酸的谷氨酸棒杆菌工程菌株[J]. 生物技术通报, 2023, 39(9): 268-280.
[2]	刘佳慧, 刘叶, 花尔并, 王猛. 谷氨酸棒杆菌中胞嘧啶碱基编辑工具的PAM拓展[J]. 生物技术通报, 2023, 39(9): 49-57.
[3]	李仁瀚, 张乐乐, 刘春立, 刘秀霞, 白仲虎, 杨艳坤, 李业. 基于紫色杆菌素生物合成途径的L-色氨酸生物传感器的构建[J]. 生物技术通报, 2023, 39(10): 80-92.
[4]	胡海洋, 应婉琴, 何军, 吕芷贤, 谢小平, 邓仲良. 酶促重组等温扩增实时荧光法快速检测肺炎支原体方法的建立及应用[J]. 生物技术通报, 2022, 38(9): 264-270.
[5]	李佳乐, 林晟豪, 许文涛. 基于环介导等温扩增的抗虫基因超灵敏比色生物传感器构建[J]. 生物技术通报, 2022, 38(8): 69-76.
[6]	王鹏飞, 杨敏, 朱龙佼, 许文涛. 基于铂纳米团簇的生物传感研究进展[J]. 生物技术通报, 2021, 37(12): 235-242.
[7]	郑淑娟, 仝涛, 许文涛, 黄昆仑. 巯基-烯点击反应介导的生物传感研究进展[J]. 生物技术通报, 2021, 37(12): 243-251.
[8]	李信申, 黄小梅, 吴淑秀, 黄瑞荣, 魏林根, 华菊玲. 植物青枯病菌环介导等温扩增快速检测技术研究[J]. 生物技术通报, 2021, 37(1): 272-281.
[9]	赵颖, 王楠, 陆安祥, 冯晓元, 郭晓军, 栾云霞. 核酸适配体侧流层析分析技术在真菌毒素检测中的应用[J]. 生物技术通报, 2020, 36(8): 217-227.
[10]	方顺燕, 宋丹, 刘艳萍, 徐文娟, 刘佳瑶, 韩向峙, 龙峰. 用于Escherichia coli O157∶H7直接快速检测的倏逝波荧光核酸适配体传感器研究[J]. 生物技术通报, 2020, 36(7): 228-234.
[11]	叶健文, 陈江楠, 张旭, 吴赴清, 陈国强. 动态调控：一种高效的细胞工厂工程化代谢改造策略[J]. 生物技术通报, 2020, 36(6): 1-12.
[12]	杨敏, 李舒婷, 杨文平, 李相阳, 许文涛. DNA/银纳米簇介导的功能核酸生物传感器研究进展[J]. 生物技术通报, 2020, 36(6): 245-254.
[13]	高威芳, 章礼平, 朱鹏. 等温扩增技术及其结合CRISPR在微生物快速检测中的研究进展[J]. 生物技术通报, 2020, 36(5): 22-31.
[14]	柳苏月, 田晶晶, 田洪涛, 许文涛. 铽（III）离子及其复合物：从发光特性到传感应用[J]. 生物技术通报, 2020, 36(4): 192-207.
[15]	孙雨阁, 李宸葳, 杜再慧, 许文涛. FEN1酶介导的功能核酸生物传感器的研究进展[J]. 生物技术通报, 2020, 36(4): 208-224.