生物技术通报 ›› 2021, Vol. 37 ›› Issue (9): 266-273.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0015

• 技术与方法 • 上一篇    下一篇

CRISPR-Cas系统在核酸检测中的应用研究

胡秀文1(), 刘华2,4, 王宇3, 唐雪明1,5(), 王金斌2,4, 曾海娟2,4, 蒋玮2,4, 李红6   

  1. 1.上海海洋大学食品学院,上海 201306
    2.上海农业科学院生物技术研究所,上海 201106
    3.兰州理工大学生命科学与工程学院,兰州 730050
    4.上海市农业遗传育种重点实验室,上海 201106
    5.上海交通大学农业与生物学院,200240
    6.上海市农业科学院畜牧兽医研究所,上海 201106
  • 收稿日期:2021-01-04 出版日期:2021-09-26 发布日期:2021-10-25
  • 作者简介:胡秀文,女,硕士研究生,研究方向:分子生物学;E-mail: 849727644@qq.com
  • 基金资助:
    上海市科委自然科学基金项目(19ZR1436800);上海市农业科学院卓越团队计划农科创(2017B-07)

Application of CRISPR-Cas System in Nucleic Acid Detection

HU Xiu-wen1(), LIU Hua2,4, WANG Yu3, TANG Xue-ming1,5(), WANG Jin-bin2,4, ZENG Hai-juan2,4, JIANG Wei2,4, LI Hong6   

  1. 1. College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306
    2. Biotech Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201106
    3. School of Life Science and Engineering,Lanzhou University of Technology,Lanzhou 730050
    4. Shanghai Key Laboratory of Agricultural Genetics and Breeding,Shanghai 201106
    5. School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240
    6. Institute of Animal Husbandry & Veterinary Science,Shanghai Academy of Agricultural Sciences,Shanghai 201106
  • Received:2021-01-04 Published:2021-09-26 Online:2021-10-25

摘要:

CRISPR-Cas系统是一种高效、实用的基因编辑工具,被广泛应用于基因组编辑和调控机制研究。目前发现的Cas系列核酸酶工具为开发用于各种目的的不同类型核酸检测,具有广阔的应用前景。CRISPR-Cas系统的高灵敏度和高特异性,使其在病原体检测、单核苷酸多态性(SNP)分析以及基因突变检测等发挥了极其重要的作用;另外,CRISPR-Cas系统在核酸检测领域的精确、高效性,间接推动了基础生物学和应用生物学研究的进展。概述了不同类型的靶向特定核酸检测的CRISPR-Cas系统的最新进展和用途,以及与之对应的新一代的体外检测平台,旨为核酸检测领域提供新的研究思路和理论依据。

关键词: 基因编辑, CRISPR-Cas系统, Cas蛋白核酸酶, 核酸检测

Abstract:

CRISPR-Cas is an efficient and practical gene editing tool,which is widely used in genome editing and regulation. Due to its high sensitivity and specificity,the CRISPR-Cas system also plays an important role in nucleic acid detection. It can detect pathogens,analyze single nucleotide polymorphisms(SNPs),and detect genetic mutations. The currently discovered Cas series nuclease tools open the door to the development of new strategies for different types of nucleic acid detection for various purposes. In addition,the CRISPR-Cas system is used in nucleic acid detection. The accuracy and efficiency of the field indirectly promote the progress of basic biology and applied biology research. The article outlines the latest developments and uses of different types of CRISPR-Cas systems that target specific nucleic acid detection,and the corresponding new generation of in vitro detection platforms,which provide new research ideas and theoretical basis for the field of nucleic acid detection.

Key words: gene editing, CRISPR-Cas system, Cas protein nuclease, nucleic acid detection