七味白术散总苷对菌群失调腹泻小鼠肠道微生物及酶活性的影响

doi:10.13560/j.cnki.biotech.bull.1985.2021-0149

摘要/Abstract

摘要：

为揭示七味白术散治疗菌群失调腹泻的物质基础,从七味白术散中提取总苷,探讨了不同剂量的七味白术散总苷对菌群失调腹泻小鼠肠道微生态的影响。结果显示七味白术散总苷有助于恢复菌群失调腹泻小鼠的摄食量及体重。中剂量的七味白术散总苷使菌群失调腹泻小鼠的脾脏指数回到正常水平。不同剂量的七味白术散总苷均可促进双歧杆菌、乳酸菌及大肠杆菌的增殖,其中低剂量的七味白术散总苷对双歧杆菌（P<0.01）及乳酸菌（P<0.05）的增殖效果显著。不同剂量的七味白术散总苷可提高菌群失调腹泻小鼠肠道微生物活度（P<0.01）、乳糖酶（P<0.01）及蔗糖酶活性（P<0.01或P<0.05）。研究表明七味白术散总苷对菌群失调腹泻小鼠肠道有益菌、微生物活度及酶活性的影响与水煎液一致,是七味白术散治疗菌群失调腹泻的重要药效成分。

关键词: 七味白术散总苷, 肠道微生物, 微生物活度, 酶活性

Abstract:

To explore the material basis of Qiwei Baizhu Powder（QWBZP）in dysbacteria diarrhea treatment,the total glycosides（TG）from QWBZP was extracted,and the effects of TG on the intestinal microecology of diarrhea mice were investigated. The results showed that TG was conducive to restoring the food intake and body weight of diarrhea mice. TG in medium dose helped the spleen index restored to normal level. All doses of TG promoted the proliferation of Bifidobacterium,Lactobacillus and Escherichia coli,and TG in a low dose significantly promoted the proliferation of Bifidobacterium（P<0.01）and Lactobacillus（P<0.05）. All doses of TG increased intestinal microbial activities（P<0.01）,lactase activities（P<0.01）and sucrase activities（P<0.01 or P<0.05）in the mice with diarrhea. This study revealed that the effects of TG on beneficial bacteria,intestinal microbial activities and enzyme activities of the diarrhea mice were similar to that by the decoction of QWBZP. Glycosides are important pharmacological component of QWBZP in dysbacteria diarrhea treatment.

Key words: total glycosides of Qiwei Baizhu Powder, intestinal microbiota, intestinal microbial activity, enzyme activity

谢果珍, 唐圆, 吴仪, 黄莉莉, 谭周进. 七味白术散总苷对菌群失调腹泻小鼠肠道微生物及酶活性的影响[J]. 生物技术通报, 2021, 37(12): 124-131.

XIE Guo-zhen, TANG Yuan, WU Yi, HUANG Li-li, TAN Zhou-jin. Effects of Total Glycosides of Qiwei Baizhu Powder on Intestinal Microbiota and Enzyme Activities in Diarrhea Mice[J]. Biotechnology Bulletin, 2021, 37(12): 124-131.

图/表 7

图1 七味白术散总苷对菌群失调腹泻小鼠摄食量的影响 N:正常组;R:自然恢复组;TW:七味白术散水煎液组;TL:七味白术散总苷低剂量组;TM:七味白术散总苷中剂量组;TH:七味白术散总苷高剂量组,下同。横坐标时期1:适应期;2:造模期;3:治疗期

Fig. 1 Effects of total glycosides of QWBZP on the food intake of mice with dysbacteria-diarrhea N:Normal group. R:Recovery group. TW:QWBZP（Qiwei Baizhu Powder）decoction group. TL:Low-dose glycosides group. TM:Medium-dose glycosides group. TH:High-dose glycosides group,the same below. In horizontal ordinate,1:Adaptive phase. 2:Modeling phase. 3:Treatment phase

表1 七味白术散总苷对菌群失调腹泻小鼠体重的影响

Table 1 Effects of total glycosides of QWBZP on the body weight of mice with dysbacteria-diarrhea（n = 5）

分组Group	初始体重Initial weight/g	增重率 Weight gain rate/%
分组Group	初始体重Initial weight/g	适应期Adaptive phase	造模期Modeling phase	治疗期Treatment phase
N	18.57 ± 0.95	35.19 ± 7.12	20.59 ± 7.55	6.80 ± 2.69
R	18.34 ± 0.64	34.66 ± 4.94	12.20 ± 4.12	14.55 ± 2.53 A
TW	18.20 ± 0.48	38.78 ± 2.84	14.35 ± 6.09	11.36 ± 8.66
TL	18.17 ± 0.41	34.95 ± 1.41	15.43 ± 8.54	9.92 ± 3.36
TM	17.81 ± 0.83	34.74 ± 3.49	15.11 ± 6.55	11.18 ± 9.78
TH	18.01 ± 0.74	37.18 ± 4.56	13.36 ± 7.34	9.70 ± 10.13

表2 七味白术散总苷对菌群失调腹泻小鼠脏器指数的影响

Table 2 Effects of total glycosides of QWBZP on the visceral index of mice with dysbacteria-diarrhea（n = 5）

分组 Group	脾脏指数 Spleen index/%	胸腺指数 Thymus index/%	肝脏指数 Liver index/%
N	0.30 ± 0.06	0.38 ± 0.04	5.57 ± 0.29
R	0.38 ± 0.04	0.37 ± 0.08	5.30 ± 0.29
TW	0.31 ± 0.03 b	0.41 ± 0.03	5.21 ± 0.69
TL	0.39 ± 0.10	0.35 ± 0.07	5.16 ± 0.45
TM	0.33 ± 0.05	0.42 ± 0.05	5.08 ± 0.36
TH	0.41 ± 0.03 AC	0.41 ± 0.05	5.58 ± 0.34

表3 七味白术散总苷对菌群失调腹泻小鼠肠道微生物的影响

Table 3 Effects of total glycosides of QWBZP on the intestinal microbiota of mice with dysbacteria-diarrhea（n = 3）

分组 Group	细菌 Bacteria /（10⁶CFU·g^-1）	大肠杆菌 Escherichia coli /（10⁶CFU·g^-1）	双歧杆菌 Bifidobacterium /（10⁷CFU·g^-1）	乳酸菌 Lactobacillus /（10⁷CFU·g^-1）
N	1.10 ± 0. 10	0.81 ± 0.07	2.79 ± 0.82	4.53 ± 1.14
R	2.63 ± 0.21 A	0.97 ± 0.06 a	0.57 ± 0.08 a	0.65 ± 0.09 a
TW	2.04 ± 0.15 Ab	0.84 ± 0.02 b	0.60 ± 0.05 a	1.22 ± 0.78 a
TL	0.34 ± 0.11 ABC	9.09 ± 0.30 ABC	2.40 ± 0.04 BC	8.20 ± 2.80 bc
TM	15.37 ± 0.50 ABCD	2.90 ± 0.07 ABCD	2.70 ± 1.99	5.37 ± 0.35 BC
TH	2.83 ± 1.20 dE	1.21 ± 0.07 AbCDE	2.80 ± 1.84	0.96 ± 0.07 aBdE

图2 七味白术散总苷对菌群失调腹泻小鼠肠道微生物活度的影响

Fig. 2 Effects of total glycosides of QWBZP on the intes-tinal microbial activities of mice with dysbacteria-diarrhea（n=3）

图3 七味白术散总苷对菌群失调腹泻小鼠肠道乳糖酶活性的影响

Fig. 3 Effects of total glycosides of QWBZP on the lactase activities of mice with dysbacteria-diarrhea（n=3）

图4 七味白术散总苷对菌群失调腹泻小鼠肠道蔗糖酶活性的影响

Fig.4 Effects of total glycosides of QWBZP on the sucrase activities of mice with dysbacteria-diarrhea（n=3）

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