生物技术通报 ›› 2022, Vol. 38 ›› Issue (3): 1-8.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0499
• 研究报告 • 下一篇
周娟(
), 阎晋东, 李新梅, 刘雪晴, 赵强, 赵小英()
收稿日期:2021-04-16
出版日期:2022-03-26
发布日期:2022-04-06
作者简介:周娟,女,硕士研究生,研究方向：植物分子生物学;E-mail： 基金资助:
ZHOU Juan(), YAN Jin-dong, LI Xin-mei, LIU Xue-qing, ZHAO Qiang, ZHAO Xiao-ying()
Received:2021-04-16
Published:2022-03-26
Online:2022-04-06
摘要：
F-box蛋白FLAVIN-BINDING KELCH REPEAT F-BOX 1（FKF1）参与调控拟南芥光周期开花,但其分子机制尚不完全清楚。本研究通过体内和体外实验,证明FKF1与转录因子FRUITFULL（FUL）相互作用。qRT-PCR和Western blot结果显示,FKF1正调节FUL的转录水平,但不影响FUL蛋白的稳定性。遗传分析结果显示,35S-FKF1-Myc / ful-8双突变体的开花表型以及开花基因FLOWERING LOCUS T（FT）的转录水平与ful-8突变体相一致。研究结果表明FKF1可通过与FUL互作,在FUL的上游促进FT表达,进而促进开花。
周娟, 阎晋东, 李新梅, 刘雪晴, 赵强, 赵小英. 拟南芥F-box蛋白FKF1与转录因子FUL互作调控开花研究[J]. 生物技术通报, 2022, 38(3): 1-8.
ZHOU Juan, YAN Jin-dong, LI Xin-mei, LIU Xue-qing, ZHAO Qiang, ZHAO Xiao-ying. Study on the Interaction of F-box Protein FKF1 and Transcription Factor FUL in Regulating Flowering in Arabidopsis[J]. Biotechnology Bulletin, 2022, 38(3): 1-8.
| 基因座Locus | 蛋白名称Name of protein | 分值Score | 功能Function |
|---|---|---|---|
| AT1G22770 | Gigantea protein（GI） | 0.954 | Long day pathway |
| AT1G04400 | Cryptochrome 2（CRY2） | 0.952 | Light perception |
| AT5G60910 | AGAMOUS-like 8（FUL） | 0.946 | Floral promoter |
| AT3G42830 | RING/U-box superfamily protein（RBX1b） | 0.938 | |
| AT2G45660 | AGAMOUS-like 20（SOC1） | 0.9 | Floral promoter |
| AT5G20570 | RING-box 1（RBX1a） | 0.876 | |
| AT5G15840 | B-box type zinc finger protein with CCT domain（CO/BBX1） | 0.87 | Long day pathway |
| AT1G10940 | Protein kinase superfamily protein（SNRK1A） | 0.766 | |
| AT2G32950 | Transducin/WD40 repeat-like superfamily protein（COP1） | 0.726 | |
| AT1G09570 | Phytochrome A（PhyA） | 0.614 | Light perception |
| AT2G18790 | Phytochrome B（PhyB） | 0.57 | Light perception |
| AT5G11260 | Basic-leucine zipper（bZIP）transcription factor family protein（HY5） | 0.512 | |
| AT4G16250 | Phytochrome D（PhyD） | 0.87 | Light perception |
| AT2G25930 | Hydroxyproline-rich glycoprotein family protein（ELF3） | 0.878 | Circadian clock |
| AT2G46830 | Circadian clock associated 1（CCA1） | 0.608 | Circadian clock |
| AT1G09530 | Phytochrome interacting factor 3（PIF3） | 0.6 | Light signaling |
| AT5G61380 | CCT motif-containing response regulator protein（TOC1） | 0.888 | Circadian clock |
表1 FKF1候选互作蛋白
Table 1 Candidate interaction proteins of FKF1
| 基因座Locus | 蛋白名称Name of protein | 分值Score | 功能Function |
|---|---|---|---|
| AT1G22770 | Gigantea protein（GI） | 0.954 | Long day pathway |
| AT1G04400 | Cryptochrome 2（CRY2） | 0.952 | Light perception |
| AT5G60910 | AGAMOUS-like 8（FUL） | 0.946 | Floral promoter |
| AT3G42830 | RING/U-box superfamily protein（RBX1b） | 0.938 | |
| AT2G45660 | AGAMOUS-like 20（SOC1） | 0.9 | Floral promoter |
| AT5G20570 | RING-box 1（RBX1a） | 0.876 | |
| AT5G15840 | B-box type zinc finger protein with CCT domain（CO/BBX1） | 0.87 | Long day pathway |
| AT1G10940 | Protein kinase superfamily protein（SNRK1A） | 0.766 | |
| AT2G32950 | Transducin/WD40 repeat-like superfamily protein（COP1） | 0.726 | |
| AT1G09570 | Phytochrome A（PhyA） | 0.614 | Light perception |
| AT2G18790 | Phytochrome B（PhyB） | 0.57 | Light perception |
| AT5G11260 | Basic-leucine zipper（bZIP）transcription factor family protein（HY5） | 0.512 | |
| AT4G16250 | Phytochrome D（PhyD） | 0.87 | Light perception |
| AT2G25930 | Hydroxyproline-rich glycoprotein family protein（ELF3） | 0.878 | Circadian clock |
| AT2G46830 | Circadian clock associated 1（CCA1） | 0.608 | Circadian clock |
| AT1G09530 | Phytochrome interacting factor 3（PIF3） | 0.6 | Light signaling |
| AT5G61380 | CCT motif-containing response regulator protein（TOC1） | 0.888 | Circadian clock |
图1 酵母双杂交实验显示FKF1与FUL和GI相互作用
Fig.1 Yeast two-hybrid assay showing the interactions of FKF1 with FUL and GI
图2 双分子荧光互补（BiFC）和免疫共沉淀（Co-IP）实验分析FKF1与FUL的相互作用 A：BiFC 结果显示FKF1与FUL在拟南芥原生质体细胞核中相互作用。Bar = 50 μm;B：Co-IP结果显示FKF1与FUL存在相互作用
Fig. 2 BiFC and Co-IP assays showing FKF1 interaction with FUL A：BiFC assay showing the interaction of FKF1 with FUL in nucleus in Arabidopsis protoplast. Bar = 50μm. B：Co-IP assay showing the interaction of FKF1 with FUL
图3 FKF1对FUL蛋白稳定性的影响 A：FUL蛋白的半体内稳定性分析。将原核表达纯化得到的GST-FUL蛋白与野生型Col,fkf1-1或者fkf1-t 12 d苗龄幼苗的总蛋白等比例混合,孵育指定的时间,取样,用抗GST抗体进行免疫印迹检测。丽春红染色作为内标。进行了3次独立的实验,显示出相似的结果。B：（A）中GST-FUL的蛋白水平分析。利用丽春红染色信号将GST-FUL蛋白信号进行标准化。将开始取样时间点的值设定为1。误差棒代表着3个生物学重复标准偏差。C：FUL蛋白体内稳定性分析。将FUL-Flag和不同浓度FKF1-Myc在烟草叶片中共表达,提取总蛋白,用抗Flag和抗Myc抗体进行免疫印迹检测。数字表示共转化所用农杆菌的比例。丽春红染色作为内标。进行了3次独立的实验,显示出相似的结果
Fig. 3 Effect of FKF1 on FUL protein stability A：Semi-in vivo stability analysis of FUL protein. Escherichia coli-purified GST-FUL protein was mixed with total protein from the 12 d seedlings of wild-type Col,fkf1-1 or fkf1-t in equal proportion,and incubated for the given time. Sampled,and anti-GST antibody was detected by Western blotting. Ponceau staining was used as an internal standard. Three independent experiments were conducted,showing similar results. B：Protein level of GST-FUL in（A）. GST-FUL protein level was normalized to Ponceau. The value of the starting point was set to 1. Bars refer to the standard deviations of three biological replicates. C：In vivo stability analysis of FUL protein. The FUL-Flag was co-expressed with increasing amounts of FKF1-Myc in tobacco leaves,and the total protein was extracted. The anti-Flag and anti-Myc proteins were detected by Western blotting,respectively. Numbers indicate the ratios of the concentrations of Agrobacteria used in co-infiltration. Ponceau staining was used as an internal standard. Three independent experiments were conducted,showing similar results
图4 FUL mRNA的表达受FKF1调节 A：野生型Col和fkf1-1植物中FUL mRNA表达分析;B：野生型Col和ful-8中FKF1 mRNA表达分析。将植物培养在1/2MS固体培养基上,长日照条件下生长不同天数,收集幼苗地上部分,用于qRT-PCR分析。ACTIN2作为内参基因。误差棒代表着3个生物学重复标准偏差。显著性差异：** P <0.01,*** P <0.001（Tukey检验用于检测统计学差异）
Fig. 4 FUL mRNA expression regulated by FKF1 A：mRNA expression of FUL in the wild-type Col and fkf1-1 mutant. B：mRNA expression of FKF1 in the wild-type Col and ful-8 mutant. Plants grew on 1/2 MS solid medium under long-day conditions（LDs）,and aboveground seedlings were collected for quantitative real-time PCR（qRT-PCR）analysis. ACTIN2 served as the internal control. Error bars refer to the standard deviations of three biological replicates. Significant differences are indicated：**P<0.01,***P<0.001（Tukey's least significant difference test）
图5 Col,35S-FKF1-Myc,ful-8和35S-FKF1-Myc/ful-8植物开花表型 A：在长日照条件下生长40 d左右的植物照片;B：植物从播种到抽薹开花的时间;C：植物抽薹时的莲座叶数目;D：植物中FT的mRNA水平。将植物培养在1/2MS固体培养基上,长日照条件下生长,于第17天每4 h收集幼苗地上部分,用于qRT-PCR分析。ACTIN2作为内参基因。误差棒代表3个生物学重复的标准偏差。白色/黑色棒表示照光/黑暗相位。取样的第一个时间点作为0 h。误差棒代表标准偏差（n≥10）。显著性差异：* P <0.05,** P <0.01（Tukey检验用于检测统计学差异）
Fig. 5 Flowering phenotype of Col,35S-FKF1-Myc,ful-8 and 35S-FKF1-Myc / ful-8 plants A：Images of 40 d plants grown in soil under LDs. B：The days of plant sowed to bolting and blossoming of the respective genotypes. C：Number of rosette leaves at bolting of the respective genotypes. D：mRNA expression levels of FT in the respective genotypes. Plants grew in 1/2MS solid medium under LDs,samples were collected at every 4 h on day 17 for qRT-PCR analysis. ACTIN2 served as the internal control. Error bars refers to the standard deviations of three biological replicates. The white/black bars refer to light/dark phases. The time of collecting first sample is set as 0 h. Error bar refers to standard deviations（n≥10）. Significant differences are indicated：*P<0.05 and **P<0.01（Tukey's least significant difference test）
| [1] |
Bernier G, Périlleux C. A physiological overview of the genetics of flowering time control[J]. Plant Biotechnol J, 2005, 3(1):3-16.
doi: 10.1111/pbi.2005.3.issue-1 URL |
| [2] | Fornara F, de Montaigu A, Coupland G. SnapShot：control of flowering in Arabidopsis[J]. Cell, 2010,141(3):550-550. e2. |
| [3] |
Srikanth A, Schmid M. Regulation of flowering time:all roads lead to Rome[J]. Cell Mol Life Sci, 2011, 68(12):2013-2037.
doi: 10.1007/s00018-011-0673-y pmid: 21611891 |
| [4] |
Wellmer F, Riechmann JL. Gene networks controlling the initiation of flower development[J]. Trends Genet, 2010, 26(12):519-527.
doi: 10.1016/j.tig.2010.09.001 pmid: 20947199 |
| [5] |
Kardailsky I, Shukla VK, Ahn JH, et al. Activation tagging of the floral inducer FT[J]. Science, 1999, 286(5446):1962-1965.
pmid: 10583961 |
| [6] |
Lee J, Oh M, Park H, et al. SOC1 translocated to the nucleus by interaction with AGL24 directly regulates leafy[J]. Plant J, 2008, 55(5):832-843.
doi: 10.1111/tpj.2008.55.issue-5 URL |
| [7] |
Zhang L, Chen L, Yu D. Transcription factor WRKY75 interacts with DELLA proteins to affect flowering[J]. Plant Physiol, 2018, 176(1):790-803.
doi: 10.1104/pp.17.00657 URL |
| [8] |
Lee H, Suh SS, Park E, et al. The AGAMOUS-LIKE 20 MADS domain protein integrates floral inductive pathways in Arabidopsis[J]. Genes Dev, 2000, 14(18):2366-2376.
doi: 10.1101/gad.813600 URL |
| [9] |
Yamaguchi A, Kobayashi Y, Goto K, et al. TWIN SISTER OF FT(TSF)Acts as a floral pathway integrator redundantly with FT[J]. Plant Cell Physiol, 2005, 46(8):1175-1189.
pmid: 15951566 |
| [10] |
Jang S, Torti S, Coupland G. Genetic and spatial interactions between FT, TSF and SVP during the early stages of floral induction in Arabidopsis[J]. Plant J, 2009, 60(4):614-625.
doi: 10.1111/tpj.2009.60.issue-4 URL |
| [11] |
Suárez-López P, Wheatley K, Robson F, et al. CONSTANS mediates between the circadian clock and the control of flowering in Arabidopsis[J]. Nature, 2001, 410(6832):1116-1120.
doi: 10.1038/35074138 URL |
| [12] |
Valverde F. Photoreceptor regulation of CONSTANS protein in photoperiodic flowering[J]. Science, 2004, 303(5660):1003-1006.
doi: 10.1126/science.1091761 URL |
| [13] |
Wigge PA. Integration of spatial and temporal information during floral induction in Arabidopsis[J]. Science, 2005, 309(5737):1056-1059.
doi: 10.1126/science.1114358 URL |
| [14] |
Imaizumi T, Tran HG, Swartz TE, et al. FKF1 is essential for photoperiodic-specific light signalling in Arabidopsis[J]. Nature, 2003, 426(6964):302-306.
doi: 10.1038/nature02090 URL |
| [15] |
Nelson DC, Lasswell J, Rogg LE, et al. FKF1, a clock-controlled gene that regulates the transition to flowering in Arabidopsis[J]. Cell, 2000, 101(3):331-340.
pmid: 10847687 |
| [16] |
Imaizumi T, Schultz TF, Harmon FG, et al. FKF1 F-box protein mediates cyclic degradation of a repressor of CONSTANS in Arabidopsis[J]. Science, 2005, 309(5732):293-297.
pmid: 16002617 |
| [17] |
Sawa M, Nusinow DA, Kay SA, et al. FKF1 and GIGANTEA complex formation is required for day-length measurement in Arabidopsis[J]. Science, 2007, 318(5848):261-265.
doi: 10.1126/science.1146994 URL |
| [18] |
Song YH, Smith RW, To BJ, et al. FKF1 conveys timing information for CONSTANS stabilization in photoperiodic flowering[J]. Science, 2012, 336(6084):1045-1049.
doi: 10.1126/science.1219644 URL |
| [19] |
Lee BD, Kim MR, Kang MY, et al. The F-box protein FKF1 inhibits dimerization of COP1 in the control of photoperiodic flowering[J]. Nat Commun, 2017, 8(1):2259.
doi: 10.1038/s41467-017-02476-2 URL |
| [20] |
Lee BD, Cha JY, Kim MR, et al. Light-dependent suppression of COP1 multimeric complex formation is determined by the blue-light receptor FKF1 in Arabidopsis[J]. Biochem Biophys Res Commun, 2019, 508(1):191-197.
doi: 10.1016/j.bbrc.2018.11.032 URL |
| [21] |
Yan J, Li X, Zeng B, et al. FKF1 F-box protein promotes flowering in part by negatively regulating DELLA protein stability under long-day photoperiod in Arabidopsis[J]. J Integr Plant Biol, 2020, 62(11):1717-1740.
doi: 10.1111/jipb.v62.11 URL |
| [22] |
Wang JW, Czech B, Weigel D. miR156-regulated SPL transcription factors define an endogenous flowering pathway in Arabidopsis thaliana[J]. Cell, 2009, 138(4):738-749.
doi: 10.1016/j.cell.2009.06.014 URL |
| [23] |
Ferrándiz C, Gu Q, Martienssen R, et al. Redundant regulation of meristem identity and plant architecture by FRUITFULL, APETALA1 and CAULIFLOWER[J]. Development, 2000, 127(4):725-734.
pmid: 10648231 |
| [24] |
Melzer S, Lens F, Gennen J, et al. Flowering-time genes modulate meristem determinacy and growth form in Arabidopsis thaliana[J]. Nat Genet, 2008, 40(12):1489-1492.
doi: 10.1038/ng.253 URL |
| [25] |
Mandel MA, Yanofsky MF. The Arabidopsis AGL8 MADS box gene is expressed in inflorescence meristems and is negatively regulated by APETALA1[J]. Plant Cell, 1995, 7(11):1763-1771.
pmid: 8535133 |
| [26] |
Gu Q, Ferrándiz C, Yanofsky MF, et al. The FRUITFULL MADS-box gene mediates cell differentiation during Arabidopsis fruit development[J]. Development, 1998, 125(8):1509-1517.
pmid: 9502732 |
| [27] |
Schmid M, Uhlenhaut NH, Godard F, et al. Dissection of floral induction pathways using global expression analysis[J]. Development, 2003, 130(24):6001-6012.
doi: 10.1242/dev.00842 URL |
| [28] |
Abe M, Kobayashi Y, Yamamoto S, et al. FD, a bZIP protein mediating signals from the floral pathway integrator FT at the shoot apex[J]. Science, 2005, 309(5737):1052-1056.
doi: 10.1126/science.1115983 URL |
| [29] |
Torti S, Fornara F, Vincent C, et al. Analysis of the Arabidopsis shoot meristem transcriptome during floral transition identifies distinct regulatory patterns and a leucine-rich repeat protein that promotes flowering[J]. Plant Cell, 2012, 24(2):444-462.
doi: 10.1105/tpc.111.092791 URL |
| [30] |
Yamaguchi A, Wu MF, Yang L, et al. The microRNA-regulated SBP-Box transcription factor SPL3 is a direct upstream activator of LEAFY, FRUITFULL, and APETALA1[J]. Dev Cell, 2009, 17(2):268-278.
doi: 10.1016/j.devcel.2009.06.007 pmid: 19686687 |
| [31] |
Li W, Wang HP, Yu DQ. Arabidopsis WRKY transcription factors WRKY12 and WRKY13 oppositely regulate flowering under short-day conditions[J]. Mol Plant, 2016, 9(11):1492-1503.
doi: 10.1016/j.molp.2016.08.003 URL |
| [32] |
Hyun Y, Richter R, Vincent C, et al. Multi-layered regulation of SPL15 and cooperation with SOC1 integrate endogenous flowering pathways at the Arabidopsis shoot meristem[J]. Dev Cell, 2016, 37(3):254-266.
doi: 10.1016/j.devcel.2016.04.001 URL |
| [33] |
Yoo SD, Cho YH, Sheen J. Arabidopsis mesophyll protoplasts:a versatile cell system for transient gene expression analysis[J]. Nat Protoc, 2007, 2(7):1565-1572.
doi: 10.1038/nprot.2007.199 URL |
| [34] |
Sparkes IA, Runions J, Kearns A, et al. Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants[J]. Nat Protoc, 2006, 1(4):2019-2025.
pmid: 17487191 |
| [35] |
Moon J, Lee H, Kim M, et al. Analysis of flowering pathway integrators in Arabidopsis[J]. Plant Cell Physiol, 2005, 46(2):292-299.
doi: 10.1093/pcp/pci024 URL |
| [36] |
Oda A, Fujiwara S, Kamada H, et al. Antisense suppression of the Arabidopsis PIF3 gene does not affect circadian rhythms but causes early flowering and increases FT expression[J]. FEBS Lett, 2004, 557(1/2/3):259-264.
doi: 10.1016/S0014-5793(03)01470-4 URL |
| [37] |
Kim JJ, Lee JH, Kim W, et al. The microRNA156-SQUAMOSA PROMOTER BINDING PROTEIN-LIKE3 module regulates ambient temperature-responsive flowering via FLOWERING LOCUS T in Arabidopsis[J]. Plant Physiol, 2012, 159(1):461-478.
doi: 10.1104/pp.111.192369 URL |
| [38] |
Lee JH, Yoo SJ, Park SH, et al. Role of SVP in the control of flowering time by ambient temperature in Arabidopsis[J]. Genes Dev, 2007, 21(4):397-402.
doi: 10.1101/gad.1518407 URL |
| [39] |
Mathieu J, Yant LJ, Mürdter F, et al. Repression of flowering by the miR172 target SMZ[J]. PLoS Biol, 2009, 7(7):e1000148.
doi: 10.1371/journal.pbio.1000148 URL |
| [40] |
Searle I, He Y, Turck F, et al. The transcription factor FLC confers a flowering response to vernalization by repressing meristem competence and systemic signaling in Arabidopsis[J]. Genes Dev, 2006, 20(7):898-912.
doi: 10.1101/gad.373506 URL |
| [41] |
Castillejo C, Pelaz S. The balance between CONSTANS and TEMPRANILLO activities determines FT expression to trigger flowering[J]. Curr Biol, 2008, 18(17):1338-1343.
doi: 10.1016/j.cub.2008.07.075 pmid: 18718758 |
| [42] |
Liu H, Yu X, Li K, et al. Photoexcited CRY2 interacts with CIB1 to regulate transcription and floral initiation in Arabidopsis[J]. Science, 2008, 322(5907):1535-1539.
doi: 10.1126/science.1163927 URL |
| [43] |
Kumar SV, Lucyshyn D, Jaeger KE, et al. Transcription factor PIF4 controls the thermosensory activation of flowering[J]. Nature, 2012, 484(7393):242-245.
doi: 10.1038/nature10928 URL |
| [44] |
Aerts N, de Bruijn S, van Mourik H, et al. Comparative analysis of binding patterns of MADS-domain proteins in Arabidopsis thaliana[J]. BMC Plant Biol, 2018, 18(1):131.
doi: 10.1186/s12870-018-1348-8 URL |
| [45] |
Ito S, Song YH, Imaizumi T. LOV domain-containing F-box proteins:light-dependent protein degradation modules in Arabidopsis[J]. Mol Plant, 2012, 5(3):573-582.
doi: 10.1093/mp/sss013 URL |
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