转录因子VcMYB17调控蓝莓抗旱性的功能研究

doi:10.13560/j.cnki.biotech.bull.1985.2024-1031

摘要/Abstract

摘要：

目的解析转录因子VcMYB17在蓝莓响应干旱胁迫过程中的功能，为蓝莓品种改良提供基因资源。方法利用生物信息学分析VcMYB17的基本特征。通过RT-qPCR分析VcMYB17的组织表达特异性，检测不同激素处理和干旱胁迫下VcMYB17的表达水平，以及过表达VcMYB17对抗旱应答基因表达水平的影响。瞬时转化烟草检测VcMYB17的亚细胞定位，并在酵母中验证其是否具有转录激活活性。利用农杆菌侵染获得VcMYB17-OE转基因拟南芥及蓝莓愈伤，观察转基因材料的干旱表型，并测定存活率、鲜重、失水率、相对电导率、MDA含量、POD和SOD活性。结果 VcMYB17是R2R3型MYB转录因子。VcMYB17在蓝莓叶片中高表达，外源施加ABA、GA、MeJA、SA及PEG处理均导致VcMYB17表达水平下调。VcMYB17定位于细胞核，具有转录激活活性。干旱胁迫下，过表达VcMYB17的拟南芥植株及蓝莓愈伤生长受到明显抑制，MDA积累增加，但SOD、POD活性降低；且VcMYB17-OE植株中抗旱应答基因，如AtERD1、AtERD15、AtP5CS1、AtRD29A、AtRD22和AtDREB1A的表达显著低于野生型。结论 VcMYB17是定位于细胞核的R2R3型MYB转录因子，ABA、GA、MeJA、SA及PEG处理抑制VcMYB17的表达。过表达VcMYB17抑制抗旱应答基因的表达，导致拟南芥及蓝莓愈伤的抗旱性下降。

关键词: VcMYB17, 基因功能, 蓝莓, 抗旱性, 干旱胁迫, 转基因植物

Abstract:

【Objectve】This study aims to clarify the functional mechanism of the transcription factor VcMYB17 in blueberry drought stress response, providing potential genetic resources for blueberry cultivar improvement. Method Bioinformatics methods were employed to characterize the basic properties of VcMYB17. RT-qPCR was used to analyze the tissue specificity of VcMYB17, the response of VcMYB17 to different hormones and drought stress, and the effect of overexpression of VcMYB17 on drought resistance genes in Arabidopsis thaliana. Subcellular localization of VcMYB17 was examined in transiently transformed tobacco, and its transcriptional activating activity was verified in yeast. The VcMYB17-OE transgenic blueberry callus and A. thaliana were obtained through Agrobacterium infection. Drought stress phenotypes were evaluated, including measurements of survival rate, fresh weight, water loss rate, relative conductivity, malondialdehyde (MDA) content, and peroxidase (POD), and superoxide dismutase (SOD) activities. Result VcMYB17 is an R2R3 type MYB transcription factor that is highly expressed in the leaves of blueberry. Exogenous treatment with ABA, GA, MeJA, SA and PEG causes the expressions of VcMYB17 to be downregulated. VcMYB17 is localized in the nucleus and has transcriptional activating activity. Under drought stress, the overexpression of VcMYB17 inhibits plant growth, makes plants accumulate more MDA content and decrease POD and SOD activities. Furthermore, the expressions of drought response genes such as AtERD1, AtERD15, AtP5CS1, AtRD29A, AtRD22 and AtDREB1A in VcMYB17-OE plants are significantly lower than that in wild type plants. Conclusion VcMYB17 is an R2R3 MYB transcription factor localized in the nucleus. The expression of VcMYB17 is inhibited by ABA, GA, MeJA, SA and PEG. Overexpression of VcMYB17 decreases the expression of drought response genes, resulting in reduced drought resistance in A. thaliana and blueberry callus.

Key words: VcMYB17, gene function, blueberry, resistance to drought, drought stress, transgenic plant

田琴, 刘奎, 吴翔纬, 纪媛媛, 曹一博, 张凌云. 转录因子VcMYB17调控蓝莓抗旱性的功能研究[J]. 生物技术通报, 2025, 41(4): 198-210.

TIAN Qin, LIU Kui, WU Xiang-wei, JI Yuan-yuan, CAO Yi-bo, ZHANG Ling-yun. Functional Study of Transcription Factor VcMYB17 in Regulating Drought Tolerance in Blueberry[J]. Biotechnology Bulletin, 2025, 41(4): 198-210.

图/表 8

表1 RT-qPCR引物序列

Table 1 Sequences of primers for RT-qPCR

基因名称 Gene name	上游引物 Forward primer sequence （5′‒3′）	下游引物 Reverse primer sequence （5′‒3′）
VcMYB17	ATTCTGAGGAACTTCTAATGCCTT	CCAATCACCGCCATACCCAA
VcUBC28	CCATCCACTTCCCTCCAGATTATCCAT	ACAGATTGAGAGCAGCACCTTGGA
Actin	GGTAACATTGTGCTCAGTGGTGG	AACGACCTTAATCTTCATGCTGC
AtERD1	CTTTCTCTATCAGCACGAAACG	CGGTGCGATATATTGACAATCC
AtERD15	TTCGACTTGGTACCCTGATTAC	GGAAGAAGATCAGCTACATCGA
AtP5CS1	AGCTTGATGACGTTATCGATCT	AGATTCCATCAGCATGACCTAG
AtRD22	GACTTTCGATTTTACCGACGAG	CGCTACCGGTTTTACCTTTATG
AtRD29A	TTCTGTAAGGACGACGTTTACA	CGTACTCGTTACATCCTCTGTT
AtDREB1A	CGTTTCAGGATGAGATGTGTGA	CTCATCGTGCATATAAAACGCA

表2 载体构建引物序列

Table 2 Sequences of primers for vector construction

引物名称 Primer name	序列 Sequence （5′‒3′）
VcMYB17-T-F	ATGGGGAGAAGCCCATGTTGC
VcMYB17-T-R	TTAATTATCTGCTAGCCAATCACCGCC
VcMYB17-pCAMBIA1205-F	CGCGGATCCATGGGGAGAAGCCCA
VcMYB17-pCAMBIA1205-R	CGGGGTACCGGCGGTGATTGGCTA
VcMYB17-pGBKT7-F	TGCATATGGCCATGGATGGGGAGAAGCCCA
VcMYB17-pGBKT7-R	CCGCTGCAGGTCGACATTATCTGCTAGCCA
VcMYB17-pCAMBIA1300-F	AGCTCGGTACCCGGGATGGGGAGAAGCCCA
VcMYB17-pCAMBIA1300-R	CATGTCGACTCTAGAATTATCTGCTAGCCA

图1 VcMYB17的鉴定及系统发育分析A：VcMYB17转录组数据分析；B：VcMYB17结构域分析；C：VcMYB17多序列比对；D：VcMYB17系统进化树分析。不同字母代表使用t检验方法差异显著（P<0.05），下同

Fig. 1 Identification and phylogenetic analysis of VcMYB17A: VcMYB17 transcriptome data analysis. B: VcMYB17 domain analysis. C: Multiple sequence alignment of VcMYB17. D: Phylogenetic tree analysis of VcMYB17. Different letters indicate the use of Turkey test methods significantly different (P<0.05), the same below

图2 VcMYB17的表达模式分析A：VcMYB17组织表达特异性分析；B‒E：ABA、GA、MeJA、SA处理后VcMYB17的相对表达量；F：VcMYB17对10% PEG响应情况

Fig. 2 Expression pattern analysis of VcMYB17A: Tissue expression specificity analysis of VcMYB17. B-E: The relative expression of VcMYB17 after ABA, GA, MeJA and SA treatment. F: Response of VcMYB17 to 10% PEG

图3 VcMYB17亚细胞定位及转录活性分析A：VcMYB17亚细胞定位分析，标尺=25 μm；B：VcMYB17转录活性分析

Fig. 3 Subcellular localization and transcriptional activity analysis of VcMYB17A: Subcellular localization analysis of VcMYB17, scale bar=25 μm. B: Transcriptional activity analysis of VcMYB17

图4 VcMYB17-OE拟南芥干旱表型分析及相关指标测定A：DNA水平转基因拟南芥鉴定； B：RT-qPCR转基因拟南芥鉴定；C：不同基因型拟南芥干旱表型分析；D‒I：正常及干旱情况下不同基因型拟南芥生理指标测定结果

Fig. 4 Drought phenotypic analysis of VcMYB17-OE A. thaliana and determination of related indexesA: Identification of transgenic Arabidopsis thaliana at DNA level. B: Identification of transgenic A. thaliana by RT-qPCR. C: Drought phenotype analysis of A.thaliana with different genotypes. D‒I: Results of physiological indexes of A.thaliana with different genotypes under normal and drought conditions

图5 VcMYB17-OE蓝莓愈伤干旱表型分析及相关指标测定A：VcMYB17-OE蓝莓愈伤鉴定；B：转基因愈伤干旱表型分析，bar=2 cm；C‒F：正常及5% PEG处理下生理指标测定结果

Fig. 5 Phenotypic analysis of callus and drought in VcMYB17-OE blueberry and determination of related indexesA: Identification of VcMYB17-OE blueberry callus. B: Phenotypic analysis of transgenic callus drought, bar=2 cm. C‒F: Results of physiological indexes under normal and 5% PEG treatment

图6 正常及干旱处理下不同基因型拟南芥中耐旱基因的表达情况

Fig. 6 Expressions of drought-tolerant genes in A. thaliana with different genotypes under normal and drought treatment

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