精子载体法制备转基因动物的研究进展

生物技术通报 ›› 2012, Vol. 0 ›› Issue (12): 13-18.

精子载体法制备转基因动物的研究进展

杜伟, 崔海信, 王琰, 孙长娇, 赵翔

中国农业科学院农业环境与可持续发展研究所中国农业科学院农业纳米研究中心，北京 100081

收稿日期:2012-05-22 修回日期:2013-01-25 出版日期:2012-12-26 发布日期:2013-02-06
作者简介:杜伟, 男, 硕士研究生, 研究方向:分子生物物理;E-mail:duwei_1987@126.com
基金资助:
: 转基因生物新品种培育重大专项资助项目（2009ZX08010-006B）, 中央级公益性科研院所基本科研业务费专项资金 (BSRF 201108)

Research Progress on Spermatozoa-mediated Gene Transfer to Product Transgenic Animals

Du Wei, Cui Haixin, Wang Yan, Sun Changjiao, Zhao Xiang

1Institute of Environment and Sustainable Development in Agriculture，Chinese Academy of Agricultural Sciences，Research Center forNanoscale Science in Agriculture，Chinese Academy of Agricultural Sciences，Beijing 100081

Received:2012-05-22 Revised:2013-01-25 Published:2012-12-26 Online:2013-02-06

摘要/Abstract

摘要： 精子载体法是一种低成本、简单快速制备转基因动物的方法。外源基因主要通过两种方式实现基因整合，一种是直接整合到精子的基因组中；另一种是通过精子携带进入卵细胞，然后整合到受精卵的基因组中。尽管现在已经有很多利用精子载体法获得了转基因动物的研究，但是基于精子在受精过程中，结合、内化、以及转运外源基因等方面能力的差异，转染效率仍有待提高。就利用精子载体法制备转基因动物过程中，精子载体法制备转基因动物的分子机制和方法方面进行系统的阐述和分析。

关键词: 精子载体法, 转基因, 精子

Abstract: Spermatozoa-mediated gene transfer（SMGT）is a low-cost and fast method used to produce transgenic animals. Theexogenous DNA can be either integrated into the sperm chromosomal DNA or simply transferred to the egg by the spermatozoa and laterintegrated into the zygote’s genome. Although there are many transgenic animals produced by SMGT，its efficiency is still low，mainly dueto the ability of sperm to bind，internalize，and transport exogenous DNA into an oocyte during fertilization. The purpose of this review is tosummarize what has been achieved with this method，such as the binding of DNA molecules to spermatozoa，and attempts made to producetransgenic animals in this field.

杜伟, 崔海信, 王琰, 孙长娇, 赵翔. 精子载体法制备转基因动物的研究进展[J]. 生物技术通报, 2012, 0(12): 13-18.

Du Wei, Cui Haixin, Wang Yan, Sun Changjiao, Zhao Xiang . Research Progress on Spermatozoa-mediated Gene Transfer to Product Transgenic Animals[J]. Biotechnology Bulletin, 2012, 0(12): 13-18.

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