Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (12): 97-104.doi: 10.13560/j.cnki.biotech.bull.1985.2014.12.016

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Optimization of Refluxing Extraction Technology of Anthocyanins from Purple Sweet Potato by Response Surface Method

Liu Qian, Qi Jiying, Han Jing, Wan Yue, Zheng Shibin   

  1. (School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016)
  • Received:2014-05-12 Online:2014-12-08 Published:2014-12-12

Abstract: Response surface methodology(RSM)was used to optimize the refluxing extraction process of anthocyanins from purple sweet potatoes as a result of getting the optimum technology. The results of single-factor test showed that the best ethanol concentration was 70%, extraction temperature was 60℃, extraction time was 60 min, liquid-material ratio was 7∶1. Based on these results, the significant parameters were further optimized using Box-Behnken design, RSM and regression analysis. The optimum parameters were :ethanol concentration 74%, extraction temperature 66℃, extraction time 56 min, liquid-material ratio 8∶1. Under such conditions, the extraction efficiency could reach 4.64 mg/g, and have a smaller relative error at 0.85% compared with the theoretical value(4.68 mg/g). The study showed that the processing parameters achieved from these experiments were accurate and can be applied to the extraction and production of anthocyanins from purple sweet potatoes.

Key words: Purple sweet potatoes, Anthocyanin, Refluxing extraction central, Composite design, Response surface methodology