Streptomycin-resistant Rational Screening for High-yield Neomycin Producing Strain

doi:10.13560/j.cnki.biotech.bull.1985.2014.12.029

Abstract

Abstract: NTG mutagenesis and streptomycin resistance screening was applied to screen neomycin high producing strain. A number of streptomycin-resistant(str)mutants were obtained from original strain of Streptomyces fradiae FS1109 treated with three different dosage of NTG under the selection pressure of resistance to streptomycin(the lethal concentration is 3 μg/mL). Streptomycin-resistant mutants strain Str63 was obtained with high neomycin yield by replica plating method and the neomycin productivity could be more than 50% higher and the component of C was lower than that of the original strain in the rotation-flask experiments, the rate of the positive mutation was larger than that of the negative mutation.

Key words: Streptomyces fradiae, Neomycin, Streptomycin resistance screening, Mutation

Chen Hong, Zhang Zhulan, Sun Fei, Zhang Yin, Zhou Jingming, Zheng Rong. Streptomycin-resistant Rational Screening for High-yield Neomycin Producing Strain[J]. Biotechnology Bulletin, 2014, 0(12): 173-176.

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